scholarly journals Blood Culture–Negative Cardiovascular Infection in a Patient With Multiple Sclerosis

2019 ◽  
Vol 6 (10) ◽  
Author(s):  
Cléa Melenotte ◽  
Ahmed Loukil ◽  
Audrey Rico ◽  
Hubert Lepidi ◽  
Didier Raoult
Keyword(s):  
Multiple Sclerosis ◽  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Chain Reaction ◽  
Serological Tests ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Polymerase Chain ◽  
Culture Negative

Abstract A patient with multiple sclerosis presented with seronegative C. burnetii endocarditis diagnosed using C. burnetii–specific polymerase chain reaction and fluorescence in situ hybridization on cardiovascular biopsy. This case supports the necessity of a systematic polymerase chain reaction testing of removed cardiac valves because blood culture–negative endocarditis can be pauci-symptomatic, and serological tests can be negative in cases of immunosuppression.

scholarly journals Whipple’s endocarditis: a case report of a blood culture-negative endocarditis

2019 ◽  
Vol 3 (4) ◽  
pp. 1-6
Author(s):  
Miriam A Scheurwater ◽  
Cees M Verduin ◽  
Jan-Melle van Dantzig
Keyword(s):  
Polymerase Chain Reaction ◽  
Mitral Valve ◽  
Blood Culture ◽  
Asymptomatic Carrier ◽  
Tropheryma Whipplei ◽  
Invasive Infection ◽  
Chain Reaction ◽  
Rehabilitation Centre ◽  
Polymerase Chain ◽  
Culture Negative

Abstract Background Whipple’s disease is caused by Tropheryma whipplei and causes a self-limiting gastrointestinal infection. The majority of the population is an asymptomatic carrier, however, in some patients, it causes an invasive infection with for example arthritis, endocarditis, or involvement of the eyes. Case summary This case describes a man with long-lasting complaints of progressive dyspnoea caused by heart failure due to total destruction of the aortic and mitral valve as a result of T. whipplei endocarditis, diagnosed with serum polymerase chain reaction. The patient was treated with ceftriaxone and prolonged co-trimoxazole therapy and surgical replacement of the aortic and mitral valve. He was discharged to a rehabilitation centre. Discussion Tropheryma whipplei is one of the possible microorganisms classified as causing blood culture-negative endocarditis, with predominantly afebrile patients that do not fulfil the Dukes criteria, which makes it difficult to diagnose. Polymerase chain reaction is the cornerstone of the diagnosis. It requires long-term antibiotic treatment up to 12 months. It is recommended by the European Society of Cardiology to discuss treatment in an Endocarditis Team because Whipple’s endocarditis has only rarely been described in the literature previously. Whipple’s endocarditis has high mortality and relapse rates.

Introduction of routine polymerase chain reaction testing for Neisseria gonorrhoeae in a community laboratory

Sexual Health ◽  
2013 ◽  
Vol 10 (4) ◽  
pp. 387 ◽  
Author(s):  
Arlo Upton ◽  
Janet Wilson ◽  
Liselle Bissessor
Keyword(s):  
Polymerase Chain Reaction ◽  
Neisseria Gonorrhoeae ◽  
Positive Predictive Value ◽  
Predictive Value ◽  
Positive Culture ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Additional Testing ◽  
Polymerase Chain ◽  
Culture Negative

We introduced polymerase chain reaction (PCR) testing for Neisseria gonorrhoeae (NG) on the Cobas 4800 CT/NG assay for all samples received with a Chlamdyia trachomatis request in March 2012. From 1 March 2012 to 30 June 2012, all PCR-positive/culture-negative specimens had additional testing at another assay. A total of 40053 tests were performed. The estimated specificity and positive predictive value were 99.9% and 97.1%, respectively; thus routine additional testing is not required.

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

Routine identification of four sympatric species of calanoid copepods Pseudocalanus spp. in the Atlantic Arctic using a species-specific polymerase chain reaction

2020 ◽  
Vol 48 (1) ◽  
pp. 62-72
Author(s):  
E. A. Ershova
Keyword(s):  
Polymerase Chain Reaction ◽  
Life Cycles ◽  
The Arctic ◽  
Relative Distribution ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Routine Identification ◽  
Polymerase Chain ◽  
Species Specific ◽  
Plankton Communities

Сalanoid copepods of the genus Pseudocalanus play an important role in the plankton communities of the Arctic and boreal seas, often dominating in numbers and constituting a significant proportion of the biomass of zooplankton. Despite their high presence and significance in the shelf plankton communities, species-specific studies of the biology of these are significantly hampered by extremely small morphological differences between them, especially at the juvenile stages, at which they are virtually indistinguishable. In this paper, we describe a new, routine and low-cost molecular method for identifying all Pseudocalanus species found in the Atlantic sector of the Arctic: the Arctic P. acuspes, P. minutus and the boreal P. moultoni and P. elongatus, and apply it to describe the relative distribution of these species in four locations of the Arctic and sub-Arctic. With this method, species-specific polymerase chain reaction (ssPCR), mass identification of individuals of any developmental stage, including nauplii, is possible. This method can serve as an excellent tool for studying the species-specific biology of this group, describing their life cycles, as well as monitoring changes in Arctic marine ecosystems under the influence of changing climate.

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