scholarly journals Effect of Timing of Frozen Semen Insemination on Pregnancy Rate in Mares

1996 ◽  
Vol 37 (3) ◽  
pp. 361-365
Author(s):  
T. Katila ◽  
M. Celebi ◽  
E. Koskinen
Keyword(s):  
Pregnancy Rate ◽  
Frozen Semen

scholarly journals Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

2021 ◽  
Vol 34 (2) ◽  
pp. 198-204 ◽  
Author(s):  
Suherni Susilowati ◽  
Trilas Sardjito ◽  
Imam Mustofa ◽  
Oky Setio Widodo ◽  
Rochmah Kurnijasanti
Keyword(s):  
Pregnancy Rate ◽  
Dna Fragmentation ◽  
Green Tea ◽  
Prostaglandin F2α ◽  
Green Tea Extract ◽  
Control Group ◽  
Bull Semen ◽  
Frozen Semen ◽  
Tea Extract

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

scholarly journals Estrus and pregnancy rate of Simmental-Ongole Crossbred and Ongole Grade heifer after being synchronized and inseminated

2018 ◽  
Vol 43 (4) ◽  
pp. 438
Author(s):  
S. Sutiyono ◽  
D. Samsudewa ◽  
A. Suryawijaya
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Reproductive Organs ◽  
Estrus Synchronization ◽  
Purposive Sampling ◽  
Frozen Semen ◽  
Research Population ◽  
Rectal Palpation

The aim of the study was to examine the synchronization of estrus and artificial insemination in heifers of Ongole grade and Simental-Ongole grade. The research used 19 Ongole Grade (OG) and 18 Simental-Ongole Crossbred (SOC) heifers. This research applied purposive sampling to determine the research population. The criteria to select the population were heifers which have in mature body. The normality of heifer’s reproductive organs were identified by performing rectal palpation, then 50 mg of medroxy progesterone acetate on vaginal sponge was used to synchronized estrus. The estrus heifer was inseminated using frozen semen. The observed parameters were the number of heifers with normal and abnormal reproductive organs, estrus sign and pregnancy. Data were analyzed descriptively. This study showed that OG and SOC with abnormal reproductive organs was 1 heifer (5.56%) and 7 heifers (36.84%), respectively. The responses of estrus synchronization in heifers with normal and abnormal reproductive organs were 94.17% and 100% in OG, and 100% and 42.86% in SOC. Meanwhile, the pregnancy rate was 70.59 and 0% in OG, and 50.00 and 0% in SOC. In conclusion, the number of SOC heifers with abnormal reproductive organs is higher than OG. Estrus synchronization using 50 mg medroxy progesterone acetate increase the amount of estrus and pregnancy in heifers with normal reproductive organs.

The influence of purified porcine relaxin on the reproductive performance of sows following artificial insemination with fresh or frozen semen

1991 ◽  
Vol 71 (1) ◽  
pp. 237-239 ◽  
Author(s):  
P. A. Thacker ◽  
A. D. Gooneratne ◽  
R. N. Kirkwood
Keyword(s):  
Key Words ◽  
Pregnancy Rate ◽  
Litter Size ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Pregnancy Rates ◽  
Frozen Semen

Pregnancy rates and litter sizes following insemination of sows with fresh-extended and frozen-thawed semen averaged 71 vs. 53% and 10.6 vs. 4.4, respectively, and was not affected by the addition of 12.5 μg relaxin to semen. These results indicate no relaxin mediated effect on either pregnancy rate or litter size when sows are artificially inseminated with relaxin-supplemented semen. Key words: Artificial insemination, relaxin, sows

scholarly journals Using Gum Arabic in Place of Egg Yolk as Cryoprotectant for Cryopreservation of the Buck Semen

2021 ◽  
Author(s):  
Mohamed Ali
Keyword(s):  
Pregnancy Rate ◽  
Gum Arabic ◽  
Egg Yolk ◽  
Fertility Rate ◽  
Control Group ◽  
Motile Sperm ◽  
Frozen Semen ◽  
Different Levels ◽  
Motility Rate

Background: Egg yolk (EY) is well known to be toxic for buck spermatozoa, which creates restrictions of its use in cryopreservation. Therefore, this study is to compare the effect of different levels of gum Arabic (GA) in an extender on quality and fertility of cryopreserved buck sperm. Methods: Each ejaculate of six bucks was frozen in Tris with one of concentrations of GA which contained 3, 6, 9 and 12 gm/ 100 ml in place of the EY. Control was Tris extender containing 2.5% of EY. Result: A percentages of total motile sperm (54.92%; P less than 0.05) and progressively motile sperm (26.22%; P less than 0.05) of semen was frozen in Tris containing 9% of GA. Similar to control group, the pregnancy rate of does inseminated with extender containing 9% (50.0%) were significantly higher than those of does inseminated with extender containing 6% (8.33%), 3% (0.0%) and 12% (0.0%). Semen evaluation and fertility rate were similar when replacing the EY with GA in the Tris cryodiluent, after cryopreservation of buck semen. The present study shows that high motility rate of frozen semen and acceptable pregnancy rate can be obtained when using GA in place of EY for cryopreserving the buck sperm.

7 LAPAROSCOPIC INTRAUTERINE AND INTRAPERITONEAL INSEMINATION IN FAT-TAILED EWES

2013 ◽  
Vol 25 (1) ◽  
pp. 151
Author(s):  
A. Niasari-Naslaji ◽  
V. Akbarinejad ◽  
K. Hoseinipajooh ◽  
G. Akbari ◽  
S. Gharibi ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Intrauterine Insemination ◽  
Dominant Follicle ◽  
Native Breed ◽  
Frozen Semen ◽  
Significant Difference ◽  
Serum Gonadotropin ◽  
And Control ◽  
Experimental Group ◽  
Fresh Semen

The present study was conducted to evaluate laparoscopic intrauterine and intraperitoneal AI in a fat-tailed sheep breed. Multiparous and nulliparous (n = 72) healthy Shall ewes (Iranian native breed) were assigned into 3 experimental groups to be inseminated with 3 laparoscopically insemination methods, including intrauterine insemination with frozen semen (IUIFZ; n = 34), intraperitoneal insemination with fresh semen (IPIFR; n = 21), and intraperitoneal insemination with frozen semen (IPIFZ; n = 17). To perform the experiment easily and control confounding factors, the experiment was conducted in 5 replicates. In each replicate, similar numbers of ewes belonging to each experimental group were included, synchronized, and inseminated with the fresh or frozen semen collected from the same fertile ram. Estrus was synchronized using CIDR for 14 days. Immediately after CIDR removal, all ewes received 400 IU of pregnant mare serum gonadotropin. Inseminations with 300 million total spermatozoa (≥70% progressive forward motility) were performed 51.1 ± 0.64 h after CIDR removal. Intrauterine insemination was conducted at the tip of the uterine horn, ipsilateral to the ovary having a dominant follicle. In the case that both ovaries had a dominant follicle, half of the straw was deposited in each horn. In 2 other experimental groups (IPIF and IPIR) either fresh or frozen semen was deposited on the surface of ovaries having a dominant follicle. Pregnancy was diagnosed with transrectal ultrasonography on Day 30 after AI. Data were analyzed using logistic regression. The pregnancy rate in the IUIFZ group (51.7%) was significantly higher than in IPIFR and IPIFZ experimental groups (17.6 and 0%, respectively; P < 0.01), and there was significant difference between IPIFR and IPIFZ in pregnancy rate (P < 0.05). In conclusion, the laparoscopic intrauterine insemination method can be applied effectively in Shall fat-tailed sheep. Laparoscopic intraperitoneal inseminations with fresh or with frozen semen are not recommended for AI in fat-tailed sheep.

19 FACTORS AFFECTING PREGNANCY RATE AFTER ARTIFICIAL INSEMINATION WITH EQUINE SEMEN: PRODUCTION OF THE FIRST FOAL USING FROZEN SEMEN IN KOREA

2011 ◽  
Vol 23 (1) ◽  
pp. 116
Author(s):  
Y. S. Park ◽  
Y. G. Cho ◽  
K. H. Cho ◽  
G. J. Cho
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Technology Development ◽  
Skim Milk ◽  
Development Program ◽  
Field Trials ◽  
Pregnancy Rates ◽  
Chi Square ◽  
Semen Collection ◽  
Frozen Semen

Research in the area of equine artificial insemination (AI) has led to its increased application in field trials (Loomis and Graham, 2008). However, procedures for equine semen collection, cooling and freezing of semen and artificial insemination need further improvement. This study examined the effect of semen preparation and ovulation on equine AI success. Stallion semen was collected via artificial vagina, diluted with skim-milk extender, and preserved by either cooling to 4°C or cryopreservation and storage in liquid nitrogen. Mares were examined by ultrasound for follicle development with every 12 h during oestrus. During AI, semen was inserted into uterine horn adjacent to the ovulating follicle sized over 4 cm and irregular shape. In experiment 1, mares were inseminated with either cooled, cooled-transported, or frozen–thawed semen. Cooled semen was used immediately after collection and cooling. Cooled-transported semen was used 4 h after semen collection and dilution. Semen freezing procedure was followed by Moore et al. (2006). In experiment 2, we examined the effect of buffer (skim-milk extender), which was infused into the uterus at the time of AI with frozen–thawed semen. In experiment 3, we compared AI pregnancy rates for mares ovulating spontaneously v. after treatment with hCG. Pregnancy data were analysed Chi square test where P-values < 0.05 were considered significantly different. In experiment 1, the pregnancy rates after AI using cooled, cooled-transported, and frozen–thawed semen were 60 (10/6), 50 (8/4), and 37.5% (8/3), respectively, and similar among treatments. In experiment 2, the pregnancy rate of mares infused with buffer at AI was 37.5% (8/3) which was higher than that with no buffer (0%; 6/0). In experiment 3, the pregnancy rates of mares were similar between ovulated spontaneously (25%; 8/2) and ovulated with hCG (50%; 8/4). The results suggest that equine semen that has been cooled, cooled and transported or frozen can be successfully used to establish AI pregnancy. Also, the pregnancy rates after AI can be increased by infusing buffer into the uterus at AI or by inducing ovulation with hCG. These experiments resulted in the first foal produced in Korea with frozen–thawed equine semen. This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

PERIOVULATORY INSEMINATION WITH FRESH OR FROZEN SEMEN ON EMBRYO VIABILITY and EARLY PREGNANCY RATE IN GILTS

1996 ◽  
Vol 31 (2) ◽  
pp. 307-308
Author(s):  
G.R. Bertani ◽  
I.R. Scheid ◽  
F.B. Fialho ◽  
M.I.B. Rubin ◽  
I. Wentz ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Early Pregnancy ◽  
Embryo Viability ◽  
Frozen Semen

Artificial insemination by donor: a review of 12 years' experience

1982 ◽  
Vol 14 (3) ◽  
pp. 253-262 ◽  
Author(s):  
G. L. Foss
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Live Births ◽  
Future Developments ◽  
Frozen Semen

SummaryTwelve years' experience of AID in a non-NHS clinic is reviewed. Of 381 women treated, 230 became pregnant at least once; 308 pregnancies were achieved in 450 courses of treatment (68%) resulting in 263 live births (85%). For women aged over 35 years the pregnancy rate was 47%. Timing of insemination and treatment with clomiphene are described. The use of fresh or frozen semen and future developments are discussed.

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