scholarly journals Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

2021 ◽  
Vol 34 (2) ◽  
pp. 198-204 ◽  
Author(s):  
Suherni Susilowati ◽  
Trilas Sardjito ◽  
Imam Mustofa ◽  
Oky Setio Widodo ◽  
Rochmah Kurnijasanti
Keyword(s):  
Pregnancy Rate ◽  
Dna Fragmentation ◽  
Green Tea ◽  
Prostaglandin F2α ◽  
Green Tea Extract ◽  
Control Group ◽  
Bull Semen ◽  
Frozen Semen ◽  
Tea Extract

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production

2020 ◽  
Vol 21 (1) ◽  
pp. 31-35
Author(s):  
Basma El-Desoky ◽  
Shaimaa El-Sayed ◽  
El-Said El-Said
Keyword(s):  
Gene Expression ◽  
Single Dose ◽  
Green Tea ◽  
Serum Testosterone ◽  
Green Tea Extract ◽  
Male Rats ◽  
Controlled Study ◽  
Control Group ◽  
Testicular Damage ◽  
Tea Extract

Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats. Design: Randomized controlled study. Animals: 40 male Wistar rats. Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels. Results: CdCl2 decreased serum testosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats. This CdCl2-promoted testicular dysfunction was corrected via the administration of GTE to male rats. Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-induced testicular damage in male rats.

scholarly journals Streptococcus Alpha growth in gingivitis patient’s dental plaque after rinsing with green tea extract (Camellia Sinensis)

2007 ◽  
Vol 19 (1) ◽  
Author(s):  
Isti Endah Kurniwati ◽  
Juni Handajani ◽  
Regina TC. Tandelilin
Keyword(s):  
Treatment Group ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Dental Plaque ◽  
Health Benefit ◽  
Green Tea Extract ◽  
Control Group ◽  
Research Subjects ◽  
Significant Difference ◽  
Tea Extract

Green tea (Camellia sinensis) has been widely known as a healthy drink since long time ago. One of the substances in green tea which can give health benefit is catechin, an antibacterial substance. The purpose of this research is to know the efficacy of rinsing with green tea extract to Streptococcus alpha growth on gingivitis patient’s dental plaque. The research subjects include 30 mild gingivitis patients, and these subjects are divided into two groups: treatment group (20 patients) and control group (10 patients). Ten patients of treatment group rinse their mouth with 0.25% green tea extract and 10 other patients with 0.5% green tea extract. Meanwhile, control group use Bactidol (0.1% Hexetidine). Rinsing the mouth is performed every morning and night for five days. Sampling is conducted on the first and sixth day. Before rinsing data is analyzed using ANOVA and the result shows a significant difference. ANAVA testing then is done using proportion value. ANAVA Testing result shows that there is no significant difference among the patients in the treatment group. This result indicates that the effect of rinsing with 0.25% and 0.5% green tea extract is equivalent to the control (0.1% Hexetidine) to inhibit S. alpha growth on mild gingivitis patient’s dental plaque. From these two concentrations, it has not been known which one is the most effective concentration to inhibit S. alpha growth on mild gingivitis patient.

scholarly journals Protective Effects of Green Tea Extract against Hepatic Tissue Injury in Streptozotocin-Induced Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Ali Akbar Abolfathi ◽  
Daryoush Mohajeri ◽  
Ali Rezaie ◽  
Mehrdad Nazeri
Keyword(s):  
Green Tea ◽  
Tissue Injury ◽  
Diabetic Rats ◽  
Green Tea Extract ◽  
Hepatic Tissue ◽  
Control Group ◽  
Group 4 ◽  
Group 3 ◽  
Tea Extract ◽  
Group 1

Although diabetic hepatopathy is potentially less common, it may be appropriate for addition to the list of target organ conditions related to diabetes. This study was designed to evaluate the hepatoprotective properties of green tea extract (GTE) in STZ-induced diabetes in rats. Wistar rats were made diabetic through single injection of STZ (75 mg/kg i.p.). The rats were randomly divided into four groups of 10 animals each: Group 1, healthy control; Group 2, nondiabetics treated with GTE administered orally (1.5%, w/v); Group 3, diabetics; Group 4, diabetics treated with GTE (1.5%, w/v) for 8 weeks. Serum biomarkers were assessed to determine hepatic injury. Malondialdehyde (MDA) and reduced glutathione (GSH) contents were measured to assess free radical activity in the liver tissue. Hepatic antioxidant activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) were also determined. The biochemical findings were matched with histopathological verifications. Liver MDA content and serum levels of ALT, AST, ALP, and bilirubin in Group 3 significantly increased compared to Group 1 (P<0.05) and significantly decreased in Group 4 compared to Group 3 (P<0.05). Serum albumin level and GSH, SOD, CAT, and GSH-Px contents of the liver in Group 3 were significantly decreased compared to Group 1 (P<0.05) and were significantly increased in Group 4 compared to Group 3 (P<0.05). Histopathologically, the changes were in the same direction with biochemical findings. This study proved the hepatoprotective activity of GTE in experimentally induced diabetic rats.

scholarly journals Effect of sulcular green tea extract irrigation on experimental rabbit’s gingivitis (A histopathological study)

2020 ◽  
Vol 32 (1) ◽  
pp. 19-27
Author(s):  
Dilyar A Baban
Keyword(s):  
Experimental Study ◽  
Green Tea ◽  
Green Tea Extract ◽  
Control Group ◽  
Study Group ◽  
Distilled Water ◽  
Cell Counts ◽  
Experimental Gingivitis ◽  
Significant Difference ◽  
Tea Extract

Background: Considering the antioxidant, anti-inflammatory, and antimicrobial properties of green tea, this study aimed to evaluate the histopathological effect of the sulcular irrigation of green tea extract in the treatment of experimental gingivitis in rabbit. Materials and methods: For this experimental study, 45 male rabbits, separated in two groups, control non- irrigated group (5rabbits) and study group (40 rabbits), gingivitis induced by ligatures was packed subgingivally in the lower right central incisors of the experimental group for seven days. Then, the animals were randomly designated to two irrigated groups (20 rabbits for each) with 50µl/kg of distilled water and 5% green tea extract once daily for seven days starting from the day of ligature removal respectively. Specimens have taken at 1, 3, 7, and 14 days for light microscopical evaluation of inflammatory cellular infiltration. Results: Regarding study group (5% of green tea extract), results demonstrated a significant reduction in the mean values of inflammatory cell counts within three days (P≤ 0.05) to reach the amount of control group within seven days (P˃ 0.05). A highly significant difference was detected between control group and study group sprayed with distilled water during all healing periods (P≤ 0.05). Histological examination showed that the resolution of gingival inflammation, re-epithelization, and tissue healing were achieved much quicker in the group of gingival sulcular irrigation with 5% green tea extract. Conclusions: In this experimental study, the histopathological analysis demonstrated that the use of 5% extract of green tea as gingival sulcular irrigation might fasten healing after the induction of gingivitis. Keywords: green tea, animal model, gingival sulcular irrigation, experimental gingivitis.

The Interaction of Zinc Oxide/Green Tea Extract Complex Nanoparticles and its Effect on Monosodium Glutamate Toxicity in Liver of Rats

2019 ◽  
Vol 20 (6) ◽  
pp. 465-475 ◽  
Author(s):  
Fawziah A. Al-Salmi ◽  
Reham Z. Hamza ◽  
Nahla S. El-Shenawy
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Green Tea ◽  
Zinc Oxide Nanoparticles ◽  
Monosodium Glutamate ◽  
Green Tea Extract ◽  
Control Group ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are increasingly utilized in both industrial and medical applications. Therefore, the study was aimed to investigate the effect of green nanoparticle complex (green tea extract/zinc oxide nanoparticles complex, GTE/ZnO NPs) on oxidative stress induced by monosodium glutamate (MSG) on the liver of rats. Methods: Wistar male rats (n=64) weighing between 200-250 g were divided randomly into eight groups: control group was given physiological saline (1 mg/kg), two groups were treated with two different doses of MSG (MSG-LD, MSG-HD; 6 and 17.5 mg/Kg, respectively), GTE was given 1 mg/mL, 5th group was treated with ZnO NPs and 6th group was treated with GTE/ZnO NPs complex while, 7th and 8th groups were treated with MSG-LD + GTE/ZnO NPs complex and MSG-HD + GTE/ZnO NPs complex, respectively. All substances were given orally for 30 consecutive days. At the end of the study, the liver was homogenized for measurement of the oxidative stress status and anti-inflammatory biomarkers as well as histological and transmission alternations. Results: Results showed that the antioxidant enzymes activity and glutathione level were significantly decreased in MSG groups than control in a dose-dependent manner. Conversely, the malondialdehyde and inflammatory cytokines levels were significantly increased in MSG groups than the control group. The liver indicated no evidence of alteration in oxidative status, anti-inflammatory and morphological parameters in GTE, ZnO NPs and GTE/ZnO NPs complex groups. Conclusion: In conclusion, MSG at both doses caused oxidative stress and inflammation on liver after 28 days of exposure that supported histological analysis and transmission view of hepatic parenchyma. GTE/ZnO NPs act as partial hepato-protective against MSG.

scholarly journals PERAN TEH HIJAU TERHADAP OVARIUM, ENDOMETRIUM DAN AORTA TIKUS YANG DIPAPAR DEPOMEDROXY PROGESTERONE ACETATE (DMPA)

2021 ◽  
Vol 7 (3) ◽  
pp. 568-575
Author(s):  
Nora Veri
Keyword(s):  
Corpus Luteum ◽  
Green Tea ◽  
Green Tea Extract ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Tea Extract ◽  
True Experiment

Background: The type of injectable contraceptive that is currently widely used is Depo Medroxyprogesterone Acetate (DMPA) which contains the hormone progestin, which is a synthetic material from progesterone. The use of progestin contraceptives can reduce endometrial proliferation and increase apoptosis in endometrial tissue. Cell apoptosis is triggered by an imbalance between positive signals (cell growth factors) and negative signals (DNA damage and increased levels of oxidants) under conditions of oxidative stress. Apoptosis of oocytes in large numbers will cause tissue damage which is characterized by atrophy of the ovaries and causes decreased reproductive function. Oxidative stress can be overcome with endogenous and exogenous antioxidants to reduce free radicals in the body. Green tea is one of the herbal plants that act as antioxidants and phytoestrogens, through the polyphenol compounds contained therein.Purpose: To know the role of green tea in providing protective effects on the ovaries, endometrium and aorta in rats.Methods : The research design used in this study was a true experiment with a post test only control group design approach. In this study, the researchers treated green tea extract at a dose of 10.8 mg/rat/day, 21.6 mg/rat/day, and 43.2 mg/rat/day and exposure to DMPA at a dose of 2.7 mg/rat/day. Green tea extract was administered orally for 28 days. The research hypothesis is that green tea can increase the number of follicles, the number of endometrial glands and reduce the arteriosclerosis score of blood vessels. The preparations were stained by the Hemotoxin-Eosin (HE) method and using the OlyVIA software.Result: There was a significant decrease in the number of corpus luteum, endometrial glands and arteriosclerosis scores due to DMPA exposure but not on the number of follicles. Green tea extract was able to increase the number of endometrial glands at a dose of 10.8 mg/day, but there was no significant difference between doses. Green tea extract was able to reduce arteriosclerosis scores, but the most effective dose was 43.2 mg/day.Conclusion: DMPA exposure was able to significantly reduce the number of corpus luteum, the number of endometrial glands and arteriosclerosis score, but there was no significant difference in the number of primary, secondary, tertiary and de Graff follicles, there was a difference in the number of corpus luteum in the group exposed to DMPA, green tea extract was able to increase the number of endometrial glands and decrease the arteriosclerosis score.Suugestion It is necessary to determine the phase of the reproductive cycle before calculating the number of follicles and examining the level of the hormone estrogen to determine whether the experimental animal has become a hypoestrogen model or not. Keywords : Progesterone, Contraception, Green Tea, Antioxidant ABSTRAK Latar Belakang : Jenis kontrasepsi suntik yang saat ini banyak digunakan adalah Depo Medroxyprogesterone Acetate (DMPA) yang mengandung hormon progestin yaitu bahan sintestis dari progesteron. Penggunaan kontrasepsi progestin dapat menurunkan proliferasi endometrium dan meningkatkan apoptosis pada jaringan endometrium. Apoptosis sel dipicu karena adanya ketidakseimbangan antara sinyal positif (faktor pertumbuhan sel) dan sinyal negatif (kerusakan DNA dan peningkatan kadar oksidan) pada kondisi stress oksidatif. Apoptosis pada oosit dalam jumlah yang besar akan mengakibatkan kerusakan jaringan yang ditandai dengan terjadinya atrofi pada ovarium dan menyebabkan fungsi reproduksi menurun. Stress oksidatif dapat diatasi dengan antioksidan endogen dan eksogen untuk menurunkan radikal bebas dalam tubuh. Teh hijau merupakan salah satu tanaman herbal yang berperan sebagai antioksidan dan fitoestrogen, melalui senyawa polifenol yang terkandung didalamnya.Tujuan : Mengetahui peran teh hijau dalam meberikan efek protektif terhadap ovarium, endometrium dan aorta pada tikus.Metode : Rancangan penelitian yang digunakan dalam penelitian ini adalah true experiment dengan pendekatan post test only control group design. Dalam penelitian ini perlakuan peneliti adalah pemberian ekstrak teh hijau dengan dosis 10,8 mg/tikus/hari, 21,6 mg/tikus/hari, dan 43,2 mg/tikus/hari dan paparan DMPA dosis 2,7 mg/tikus/hari pada tikus. Ekstrak teh Hijau diberikan secara oral selama 28 hari. Hipotesis penelitian adalah teh hijau mampu meningkatkan jumlah folikel, jumlah kelenjar endometrium dan menurunkan skor arteriosklerosis pembuluh darah. Preparat diwarnai dengan metode Hemotoxin-Eosin (HE) dan menggunakan software OlyVIA.Hasil : Terjadi penurunan jumlah korpus luteum, kelenjar endometrium dan skor arteriosklerosis akibat paparan DMPA secara bermakna namun tidak pada jumlah folikel. Ekstrak teh hijau mampu meningkatkan jumlah kelenjar endometrium pada dosis 10.8 mg/hari, namun tidak ada perbedaan yang signifikan antar dosis. Ekstrak teh hijau mampu menurunkan skor arteriosklerosis, namun yang paling efektif adalah dosis 43.2 mg/hari.Kesimpulan : Paparan DMPA mampu menurunkan jumlah korpus luteum, jumlah kelenjar endometrium dan skor arteriosklerosis secara bermakna, namun tidak ada perbedaan yang bermakna pada jumlah folikle primer, sekunder, tersier dan de Graff, ada perbedaan jumlah korpus luteum pada kelompok yang dipapar DMPA, ekstrak teh hijau mampu meningkatkan jumlah kelenjar endometrium  dan menurunkan skor arteriosklerosis.Saran Perlu menentukan fase siklus reproduksi sebelum dilakukan perhitungan jumlah folikel dan pemeriksaan kadar hormon estrogen untuk menentukan apakah hewan coba sudah menjadi model hypoestrogen atau belum. Kata Kunci :  DMPA, Kontrasepsi, Teh Hijau, Antioksidan 

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