scholarly journals Gas Chromatographic Determination of Volatile Fatty Acids in Aqueous Media its use in Bovine Rumen Fluid and in Silage

1965 ◽  
Vol 6 (1) ◽  
pp. 52-58
Author(s):  
G. Carlström ◽  
W. Hallgren ◽  
B. Pehrson ◽  
O. Wallin
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Aqueous Media ◽  
Chromatographic Determination ◽  
Bovine Rumen

scholarly journals Theoretical Methane Emission Estimation from Volatile Fatty Acids in Bovine Rumen Fluid

2021 ◽  
Vol 11 (16) ◽  
pp. 7730
Author(s):  
Sang-Ryong Lee ◽  
Yunseo Cho ◽  
Hyuck K. Ju ◽  
Eunjeong Kim
Keyword(s):  
Fatty Acids ◽  
Conventional Method ◽  
Butyric Acid ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Esterification Reaction ◽  
Livestock Farming ◽  
Bovine Rumen ◽  
Ch4 Production ◽  
Improved Accuracy

Methane production from livestock farming is recognized as an important contributor to global GHGs. Volatile fatty acids (VFAs) found in bovine rumen may be utilized as a substrate for methanogens to form CH4, and thus improvement of quantitative VFA measurements can help facilitate greater understanding and mitigation of CH4 production. This study aims to contribute to the development of more accurate methods for the quantification and specification of VFAs in bovine rumen. The VFAs were analyzed using the conventional method and an alternative catalytic esterification reaction (CER) method. Substantial differences in the detected concentrations of the C3+ VFAs (chain length ≥ 3) were observed between both methods, especially for butyric acid. Evaluation of the sensitivity of both methods to detecting the VFA concentrations in standard solutions confirmed that the values resulting from the CER method were closer to the known concentrations of the standard solution than those from the conventional method. The results of this study provide the first quantitative proof to show the improved accuracy of the measurements of C3+ VFAs when using the CER method compared with the conventional method. Therefore, the CER method can be recommended to analyze the VFAs found in rumen, especially butyric acid and other C3+ VFAs.

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