scholarly journals Influence of Boar Seminal Plasma on the Distribution of Spermatozoa in the Genital Tract of Gilts

1980 ◽  
Vol 21 (4) ◽  
pp. 598-606
Author(s):  
Sven Viring ◽  
Stig Einarsson
Keyword(s):  
Seminal Plasma ◽  
Genital Tract

scholarly journals The role of semen and seminal plasma in inducing large-scale genomic changes in the female porcine peri-ovulatory tract

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
M. Álvarez-Rodríguez ◽  
C. A. Martinez ◽  
D. Wright ◽  
H. Rodríguez-Martinez
Keyword(s):  
Seminal Plasma ◽  
Genital Tract ◽  
Large Scale ◽  
Enrichment Analysis ◽  
Estrogen Signaling ◽  
Cell Recognition ◽  
Genomic Changes ◽  
Expression Of Genes ◽  
Microarray Analyses

AbstractSemen modifies the expression of genes related to immune function along the porcine female internal genital tract. Whether other pathways are induced by the deposition of spermatozoa and/or seminal plasma (SP), is yet undocumented. Here, to determine their relative impact on the uterine and tubal transcriptomes, microarray analyses were performed on the endocervix, endometrium and endosalpinx collected from pre-ovulatory sows 24 h after either mating or artificial insemination (AI) with specific ejaculate fractions containing spermatozoa or sperm-free SP. After enrichment analysis, we found an overrepresentation of genes and pathways associated with sperm transport and binding, oxidative stress and cell-to-cell recognition, such as PI3K-Akt, FoxO signaling, glycosaminoglycan biosynthesis and cAMP-related transcripts, among others. Although semen (either after mating or AI) seemed to have the highest impact along the entire genital tract, our results demonstrate that the SP itself also modifies the transcriptome. The detected modifications of the molecular profiles of the pre/peri-ovulatory endometrium and endosalpinx suggest an interplay for the survival, transport and binding of spermatozoa through, for instance the up-regulation of the Estrogen signaling pathway associated with attachment and release from the oviductal reservoir.

scholarly journals The Expression of Cold-Inducible RNA-Binding Protein mRNA in Sow Genital Tract Is Modulated by Natural Mating, But Not by Seminal Plasma

2020 ◽  
Vol 21 (15) ◽  
pp. 5333
Author(s):  
Jaume Gardela ◽  
Mateo Ruiz-Conca ◽  
Cristina A. Martinez ◽  
Dominic Wright ◽  
Manel López-Béjar ◽  
...  
Keyword(s):  
Ion Channels ◽  
Mrna Expression ◽  
Seminal Plasma ◽  
Genital Tract ◽  
Transient Receptor Potential ◽  
Rna Binding ◽  
Trp Channels ◽  
Rna Binding Proteins ◽  
Trp Ion Channels ◽  
Natural Mating

The RNA-binding proteins (RBPs), some of them induced by transient receptor potential (TRP) ion channels, are crucial regulators of RNA function that can contribute to reproductive pathogenesis, including inflammation and immune dysfunction. This study aimed to reveal the influence of spermatozoa, seminal plasma, or natural mating on mRNA expression of RBPs and TRP ion channels in different segments of the internal genital tract of oestrous, preovulatory sows. Particularly, we focused on mRNA expression changes of the cold-inducible proteins (CIPs) and related TRP channels. Pre-ovulatory sows were naturally mated (NM) or cervically infused with semen (Semen-AI) or sperm-free seminal plasma either from the entire ejaculate (SP-TOTAL) or the sperm-rich fraction (SP-AI). Samples (cervix to infundibulum) were collected by laparotomy under general anaesthesia for transcriptomic analysis (GeneChip® Porcine Gene 1.0 ST Array) 24 h after treatments. The NM treatment induced most of the mRNA expression changes, compared to Semen-AI, SP-AI, and SP-TOTAL treatments including unique significative changes in CIRBP, RBM11, RBM15B, RBMS1, TRPC1, TRPC4, TRPC7, and TRPM8. The findings on the differential mRNA expression on RBPs and TRP ion channels, especially to CIPs and related TRP ion channels, suggest that spermatozoa and seminal plasma differentially modulated both protein families during the preovulatory phase, probably related to a still unknown early signalling mechanism in the sow reproductive tract.

scholarly journals Seminal Plasma Levels of PAPP-A in Normospermic and Oligospermic Men and Tissue Localization of PAPP-A in the Male Genital Tract

1985 ◽  
Vol 14 (2-3) ◽  
pp. 253-261 ◽  
Author(s):  
J. Sjöberg ◽  
T. Wahlström ◽  
M. Seppälä ◽  
E.-M. Rutanen ◽  
R. Koistinen ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Levels ◽  
Genital Tract ◽  
Male Genital Tract ◽  
Tissue Localization ◽  
Male Genital

scholarly journals β-nerve growth factor identification in male rabbit genital tract and seminal plasma and its role in ovulation induction in rabbit does

2017 ◽  
Vol 17 (2) ◽  
pp. 442-453 ◽  
Author(s):  
Rosa M. Garcia-Garcia ◽  
Maria del Mar Masdeu ◽  
Ana Sanchez Rodriguez ◽  
Pilar Millan ◽  
Maria Arias-Alvarez ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Seminal Plasma ◽  
Genital Tract ◽  
Ovulation Induction ◽  
Nerve Growth ◽  
Male Rabbit

scholarly journals Identification of genital tract markers in the human seminal plasma using an integrative genomics approach

2012 ◽  
Vol 28 (1) ◽  
pp. 199-209 ◽  
Author(s):  
A.D. Rolland ◽  
R. Lavigne ◽  
C. Dauly ◽  
P. Calvel ◽  
C. Kervarrec ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Genital Tract ◽  
Integrative Genomics ◽  
Human Seminal Plasma

scholarly journals Pharmacovirological analyses of blood and male genital compartment in patients receiving dolutegravir + lamivudine dual therapy as a switch strategy (ANRS 167 LAMIDOL trial)

2020 ◽  
Vol 75 (6) ◽  
pp. 1611-1617
Author(s):  
Charlotte Charpentier ◽  
Gilles Peytavin ◽  
François Raffi ◽  
Charles Burdet ◽  
Roland Landman ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Seminal Plasma ◽  
Genital Tract ◽  
Dual Therapy ◽  
Plasma Drug ◽  
Male Genital Tract ◽  
Hiv Dna ◽  
Drug Concentrations ◽  
Plasma Drug Concentrations ◽  
Male Genital

Abstract Objectives To describe plasma residual HIV viraemia, cellular HIV reservoir size, blood plasma drug concentrations and their male genital tract penetration during the maintenance dual therapy dolutegravir + lamivudine. Patients and methods ANRS167 LAMIDOL enrolled 104 virologically suppressed patients to switch to dolutegravir + lamivudine. In this pharmacovirological substudy, ultrasensitive plasma viral load (USpVL) and plasma drug concentrations were measured at Day 0 (D0), Week 24 (W24) and W48 of dolutegravir + lamivudine, and HIV-DNA was measured at W−8 and W48. Semen samples were collected at D0 and W24 from 18 participants. Total and unbound blood and seminal plasma drug concentrations were measured using UPLC–MS/MS. Results Median HIV-DNA was 2.5 log10 copies/106 PBMC (IQR = 2.2–3.0, n = 100) at W−8 and 2.4 log10 copies/106 PBMC (IQR = 2.1–2.9, n = 100) at W48 (P = 0.17). The proportion of patients with undetected USpVL was 38% (n = 98), 43% (n = 98) and 49% (n = 97) at D0, W24 and W48, respectively (P = 0.08). Total and unbound plasma dolutegravir concentrations were stable between timepoints (P = 0.13) and all total plasma dolutegravir concentrations except one were adequate. Median free fraction of dolutegravir in plasma was 0.21%. Median blood plasma and seminal plasma concentrations of total dolutegravir at 24 h were 1812 ng/mL and 206 ng/mL, respectively. Median seminal plasma/blood plasma total concentration ratios were 11.6% and 2478% for dolutegravir and lamivudine, respectively. HIV-RNA (365 to 475 copies/mL) was detected in seminal plasma of one patient at D0 (5.9%) and of two patients at W24 (11.8%). Conclusions These findings add further important information regarding the effectiveness of dolutegravir + lamivudine maintenance dual therapy in terms of plasma residual viraemia, cellular reservoir size and drug penetration in the male genital tract.

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