scholarly journals Identification of genital tract markers in the human seminal plasma using an integrative genomics approach

2012 ◽  
Vol 28 (1) ◽  
pp. 199-209 ◽  
Author(s):  
A.D. Rolland ◽  
R. Lavigne ◽  
C. Dauly ◽  
P. Calvel ◽  
C. Kervarrec ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Genital Tract ◽  
Integrative Genomics ◽  
Human Seminal Plasma

Macrophage metalloelastase-12 is detectable in human seminal plasma and represents a predictor for inflammatory processes in the male genital tract

Andrologia ◽  
2014 ◽  
Vol 47 (2) ◽  
pp. 153-159 ◽  
Author(s):  
A. Urbschat ◽  
P. Paulus ◽  
I. Wiegratz ◽  
H. Beschmann ◽  
P. Hadji ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Genital Tract ◽  
Male Genital Tract ◽  
Human Seminal Plasma ◽  
Inflammatory Processes ◽  
Male Genital

Human Epididymis Protein 4 Quantification and Interaction Network Analysis in Seminal Plasma

2019 ◽  
Vol 26 (6) ◽  
pp. 458-465
Author(s):  
Krishna Kant ◽  
Anil K. Tomar ◽  
Pankaj Sharma ◽  
Bishwajit Kundu ◽  
Sarman Singh ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Binding Proteins ◽  
Interaction Network ◽  
Prostatic Acid Phosphatase ◽  
Regulatory Subunit ◽  
Plasma Samples ◽  
Human Seminal Plasma ◽  
Human Epididymis Protein 4 ◽  
Human Epididymis ◽  
Core Proteins

Background: A well-known tissue marker of ovarian cancer, Human Epididymis protein 4 (HE4) is the member of whey acidic four-disulfide core proteins family. Purified from human seminal plasma and characterized as a cross-class protease inhibitor, HE4 was proposed to shield spermatozoa against proteolytic factors. However, its exact biological function is unknown. Proteins usually function in conjunction with other proteins in the system and thus, identification and analysis of protein networks become essential to decode protein functions. Objective: This study was performed to explore possible role(s) of HE4 in reproductive physiology via identification of its interactome in human seminal plasma. Methods: HE4 binding proteins were identified through co-immunoprecipitation and MALDITOF/ MS analysis. Also, HE4 was quantified by ELISA in fertile and infertile human seminal plasma samples. Results: Ten HE4 binding proteins were identified, viz. protein phosphatase 1 regulatory subunit 21, protein kinase CLK3, Ankyrin repeat domain-containing protein36A, prostatic acid phosphatase, KIF5C, Spectrin repeat containing, nuclear envelope 1, isoform CRAf, tropomyosin 4, vezatin, utrophin and fibronectin1. This interaction network suggests that HE4 plays multiple roles, specifically in capacitation, sperm motility and maturation. Further, HE4 concentration in human seminal plasma samples was determined by Elisa. Higher HE4 expression in normozoospermia compared to azoospermia and asthenozoospermia affirms its importance in fertilization. Conclusion: Based on identified interactome, it is plausible that HE4 plays a crucial role in fertilization, specifically in sperm maturation, motility and capacitation.

scholarly journals Allergy to Human Seminal Plasma

1994 ◽  
Vol 31 (3) ◽  
pp. 298-299 ◽  
Author(s):  
Sheena Robinson ◽  
Maurice J O'Kane ◽  
David R McCluskey
Keyword(s):  
Seminal Plasma ◽  
Human Seminal Plasma
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