scholarly journals Olf1/EBF associated zinc finger protein interfered with antinuclear antibody production in patients with systemic lupus erythematosus

2010 ◽  
Vol 12 (2) ◽  
pp. R59 ◽  
Author(s):  
Xuebing Feng ◽  
Rongliang Li ◽  
Jing Huang ◽  
Huayong Zhang ◽  
Lina Zhu ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Zinc Finger ◽  
Antibody Production ◽  
Lupus Erythematosus ◽  
Antinuclear Antibody ◽  
Zinc Finger Protein ◽  
Systemic Lupus ◽  
Finger Protein

Autoantibody profiling reveals four protein candidate autoantigens associated with systemic lupus erythematosus

Lupus ◽  
2018 ◽  
Vol 27 (10) ◽  
pp. 1670-1678 ◽  
Author(s):  
J Frostegård ◽  
C Hellström ◽  
P Nilsson ◽  
A G Frostegård ◽  
S Ajeganova
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Zinc Finger Protein ◽  
Tumor Necrosis Factor Receptor ◽  
Major Complication ◽  
Intima Media Thickness ◽  
Carotid Intima Media Thickness ◽  
Systemic Lupus ◽  
Serum Samples ◽  
Disease Heterogeneity

Objectives In systemic lupus erythematosus (SLE) there are typically many autoantibodies. The disease heterogeneity could be better understood with discovery of phenotype-specific antigens targeted by autoantibodies. We here aimed to identify novel autoantigens potentially related to SLE disease and a major complication, atherosclerosis. Methods Antigen microarrays were used to profile IgG autoantibody reactivity against 77 protein fragments (20–140 amino acids (aa) long, median 89 aa) produced within the Human Protein Atlas project, in serum samples from SLE patients ( n = 107) and age- and sex-matched population-based controls ( n = 107). Common carotid intima-media thickness, plaque occurrence and echogenicity were determined by B-mode ultrasound. Results We determined significant differences between patients and controls in IgG reactivity against four proteins. In patients compared to controls, there was an increase of IgG reactivity against zinc finger protein 688 (ZNF688), early B cell factor 2 (EBF2), crystallin, alpha B (CRYAB) and tumor necrosis factor receptor superfamily member 13C (TNFRSF13C). Of these four antigens, only anti-ZNF688 was associated with carotid atherosclerosis (plaque occurrence) and vulnerable plaques in SLE. There was a weak association between anti-EBF2 and SLE disease activity but no significant associations were determined for other measured IgG reactivity. Conclusions In this discovery screening we here demonstrate new candidate autoantigens with differential reactivity (reflecting autoantibody levels) in SLE patients and in controls and in relation to atherosclerosis in SLE.

scholarly journals Antinuclear Antibody–Negative Systemic Lupus Erythematosus in an International Inception Cohort

2019 ◽  
Vol 71 (7) ◽  
pp. 893-902 ◽  
Author(s):  
May Y. Choi ◽  
Ann E. Clarke ◽  
Yvan St. Pierre ◽  
John G. Hanly ◽  
Murray B. Urowitz ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Antinuclear Antibody ◽  
Inception Cohort ◽  
Systemic Lupus

scholarly journals Exposure of HEp-2 Cells to Stress Conditions Influences Antinuclear Antibody Reactivity

2002 ◽  
Vol 9 (2) ◽  
pp. 287-294 ◽  
Author(s):  
Liping Du ◽  
Sachiko Fukushima ◽  
Annahita Sallmyr ◽  
Rolf Manthorpe ◽  
Anders Bredberg
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Dna Damage ◽  
Lupus Erythematosus ◽  
Antinuclear Antibody ◽  
Nuclear Protein ◽  
Optimal Conditions ◽  
Healthy Individuals ◽  
Systemic Lupus ◽  
Lower Reactivity ◽  
Stressed Cells

ABSTRACT This study of stress-related antinuclear antibody (ANA) reactivity was undertaken with the objective of improving clinical ANA testing. ANA was determined by parallel enzyme-linked immunosorbent assays of crude nuclear protein antigen extracted from HEp-2 cells either grown under optimal conditions (providing nonstress ANA antigen) or exposed to stress (providing stress ANA antigen). The stress stimuli used were gamma radiation (causing DNA damage) and a hypertonic environment (causing apoptosis). Signs of stress-related ANA reactivity were seen among connective tissue disease (CTD) patients (including patients with systemic lupus erythematosus; mixed CTD; calcinosis, Reynaud's phenomenon, esophageal motility disorders, sclerodactyly, and telangiectasia; scleroderma; and Sjögren's syndrome): 11% showed stress-positive ANA (i.e., a significantly stronger ANA reactivity with the extract from stressed cells), whereas 21% showed a markedly weaker reaction with the stress antigen. In contrast, among ANA screening patient sera, with no diagnosis of CTD, the fraction showing stress-positive ANA was higher (7 to 8%, depending on the type of stress) than among those showing a lower reactivity with stress antigen (1.5 to 2.5%). Only one serum among 89 (1%) tested sera from healthy individuals showed a stress-related ANA reaction. This demonstration of stress-related ANA suggests a means to improve the performance of clinical ANA testing.

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