scholarly journals QUANTITATIVE ESTIMATION OF SUCCINIC DEHYDROGENASE ACTIVITY IN A SINGLE MICROSCOPIC TISSUE SECTION

1955 ◽  
Vol 3 (1) ◽  
pp. 61-69 ◽  
Author(s):  
VITTORIO DEFENDI ◽  
BJARNE PEARSON
Keyword(s):  
Dehydrogenase Activity ◽  
Tissue Section ◽  
Quantitative Estimation ◽  
Succinic Dehydrogenase ◽  
Succinic Dehydrogenase Activity

SUCCINIC DEHYDROGENASE ACTIVITY OF THE THYROID GLAND AS AN INDICATOR OF THE ACTIVITY OF THE THYROID CELLS

1960 ◽  
Vol XXXV (I) ◽  
pp. 135-138 ◽  
Author(s):  
Antti Telkkä ◽  
K. J. Heikkilä ◽  
Väinö K. Hopsu
Keyword(s):  
Thyroid Gland ◽  
Enzymatic Activity ◽  
Dehydrogenase Activity ◽  
Guinea Pigs ◽  
Quantitative Estimation ◽  
Succinic Dehydrogenase ◽  
Succinic Dehydrogenase Activity ◽  
Thyroid Cells ◽  
Thyroid Glands ◽  
Sensitive Method

ABSTRACT The thyroid glands of guinea-pigs were stimulated by the administration of methylthiouracil. The histochemically demonstrable succinic dehydrogenase activity was determined by the quantitative tetrazolium method. A marked increase in the enzymatic activity of the stimulated thyroid glands was observed and measured. The quantitative estimation of enzymatic activity is apparently a reliable and sensitive method for demonstrating the activity of the thyroid cells.

scholarly journals EFFECT OF INFECTION WITH M. TUBERCULOSIS AND OF TUBERCULIN SHOCK ON THE SUCCINIC DEHYDROGENASE ACTIVITY OF GUINEA PIG TISSUES

1953 ◽  
Vol 98 (2) ◽  
pp. 99-105 ◽  
Author(s):  
S. N. Chaudhuri ◽  
Samuel P. Martin
Keyword(s):  
Enzyme Activity ◽  
Guinea Pig ◽  
Dehydrogenase Activity ◽  
Guinea Pigs ◽  
Normal Tissue ◽  
Succinic Dehydrogenase ◽  
Tissue Extract ◽  
Succinic Dehydrogenase Activity ◽  
H37rv Strain ◽  
Sublethal Doses

The kidney of guinea pigs infected with the H37Rv and BCG strains of M. tuberculosis showed a diminution in succinic dehydrogenase activity when measured by the tetrazolium technique. This effect was also seen in the liver and spleen of animals infected with the BCG strain. Sensitized animals showed similar results when given tuberculin in sublethal doses. The succinic oxidase was also low in the kidneys of animals infected with the H37Rv strain. The depressed enzyme activity of the tissues of infected animals could be restored to normal by addition of normal tissue extract or dialysate. This suggests that the alteration in tissue metabolism observed in tuberculosis may depend upon the loss of some as yet unidentified factor important for succinic dehydrogenase activity.

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