scholarly journals Scanning electron microscopy of type I collagen at the dentin-enamel junction of human teeth.

1993 ◽  
Vol 41 (3) ◽  
pp. 381-388 ◽  
Author(s):  
C P Lin ◽  
W H Douglas ◽  
S L Erlandsen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Type I Collagen ◽  
Immunogold Labeling ◽  
Matrix Composition ◽  
Type I ◽  
Human Teeth ◽  
Dentin Matrix ◽  
Electron Imaging ◽  
Scanning Electron

The dentin-enamel junction constitutes a unique boundary between two highly mineralized tissues with very different matrix composition and physical properties. The nature of the boundary between the ectoderm-derived enamel and mesoderm-derived dentin is not known. This study was undertaken to identify the presence, type, and distribution of collagen at the dentin-enamel junction as an initial step in understanding its structural-functional role in dental occlusion. Sections of human teeth were demineralized with 0.1 M neutral EDTA and examined by high-resolution field-emission scanning electron microscopy at low accelerating voltage. Enamel and dentin were observed to be linked by many parallel 80-120-nm diameter fibrils, which were inserted directly into the enamel mineral and also merged with the interwoven fibrillar network of the dentin matrix. Immunogold labeling for collagen was visualized by secondary electron imaging and backscatter electron imaging at low accelerating voltage. The collagen fibrils at the junctional zone as well as in the dentin matrix were identified as Type I collagen. Collagenase digestion led to loss of the fibrillar structures and prevented immunogold labeling with antibody specific to Type I collagen. Consequently, the dentin-enamel junction can be regarded as a fibril-reinforced bond which is mineralized to a moderate degree.

Immunogold labeling in scanning electron microscopy

1996 ◽  
Vol 106 (1) ◽  
pp. 31-39 ◽  
Author(s):  
R. Hermann ◽  
P. Walther ◽  
M. Müller
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Immunogold Labeling ◽  
Scanning Electron

Scanning Electron Microscopy and Secondary-Electron Imaging Microscopy

2012 ◽  
pp. 2280-2280
Author(s):  
Yimei Zhu ◽  
Hiromi Inada ◽  
Achim Hartschuh ◽  
Li Shi ◽  
Ada Della Pia ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Secondary Electron ◽  
Electron Imaging ◽  
Scanning Electron

Scanning electron microscopy investigation of tooth/filling bonding

e-Polymers ◽  
2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Joanna Siejka-Kulczyk ◽  
Małgorzata Lewandowska ◽  
Marta Raczyńska ◽  
Elżbieta Jodkowska ◽  
Krzysztof J. Kurzydłowski
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Teeth ◽  
Bonding Quality ◽  
Dental Fillings ◽  
Microscopy Investigation ◽  
Electron Microscopy Investigation ◽  
Scanning Electron Microscopy Investigation ◽  
Tissue Material ◽  
Scanning Electron

AbstractScanning electron microscopy (SEM) investigations have been conducted to determine the influence of selected material on tissue/material bonding. Extracted human teeth were used in this study. Several resin composites were tested as dental fillings. To reveal the structure of the tissue/filling interface, the teeth were cut parallel to the long axis. Bonding quality was determined by direct SEM observation and microanalysis with X-ray energy spectrometry (EDX). The investigated materials show good bonding with enamel.

scholarly journals Effects of Dentifrices Containing Nanohydroxyapatite on Dentinal Tubule Occlusion—A Scanning Electron Microscopy and EDX Study

2020 ◽  
Vol 10 (18) ◽  
pp. 6513
Author(s):  
Emilia Bologa ◽  
Simona Stoleriu ◽  
Gianina Iovan ◽  
Cristina Angela Ghiorghe ◽  
Irina Nica ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Study Groups ◽  
Control Group ◽  
Dentinal Tubule ◽  
Human Teeth ◽  
Dentinal Tubules ◽  
Mineral Deposition ◽  
Tubule Occlusion ◽  
Scanning Electron

This in vitro study evaluated the effects of dentifrices containing nano-hydroxyapatite (n-HAp) on dentinal tubule occlusion and on mineral deposition. Dentin specimens of ten human teeth were submersed for 30 s in 40% citric acid and then randomly divided into four groups (three study groups and one control group). In the study groups, the dentin samples were exposed to three different n-HAp toothpastes: Karex (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany), Biorepair Plus Sensitive (Coswell SpA, Bologna, Italy), and Dr. Wolff’s Biorepair (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany); in the control group no toothpaste was applied. All of the samples were evaluated using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis. In the control group all of the samples showed a frank and wide opening of the dentinal tubules, whereas in the study groups different degrees of tubule closure by mineral depositions were observed. Toothpastes containing n-HAp determined a significant occlusion of dentinal tubules and a significant increase of mineral deposition on the dentin surface. All three tested toothpastes showed similar results regarding the degree of dentinal tubule closure. Varying degrees of differences in calcium, phosphate, carbon, and oxygen ion concentrations among the three tested toothpastes were obtained.

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