scholarly journals Immunofluorescence localization of glycoprotein hormones in the rat anterior pituitary gland using monoclonal antibodies and polyclonal antisera.

1982 ◽  
Vol 30 (7) ◽  
pp. 645-649 ◽  
Author(s):  
R Kofler
Keyword(s):  
Staining Technique ◽  
Thyroid Stimulating Hormone ◽  
Cell Type ◽  
Rat Pituitary ◽  
Pituitary Glands ◽  
Polyclonal Antisera ◽  
Simultaneous Identification ◽  
Mouse Antibody ◽  
Monoclonal Mouse ◽  
Stimulating Hormone

A hybridoma-derived monoclonal mouse antibody against bovine luteinizing hormone (LH) cross-reacting with rat LH and conventional polyclonal rabbit antisera against rat follicle-stimulating hormone (FSH) and thyroid-stimulating hormone (TSH) were used for the localization of the cells producing these hormones in rat pituitary glands by the immunofluorescence double staining technique. This approach enables the simultaneous identification of two different hormones on a single tissue section. It was shown that LH and FSH are produced within the same cell type, whereas TSH is synthesized by a different cell.

EFFECT OF TESTOSTERONE PROPIONATE ON THE RELEASE OF FSH FROM THE PITUITARY GLAND

1968 ◽  
Vol 57 (2) ◽  
pp. 289-295 ◽  
Author(s):  
E. Steinberger ◽  
G. Duckett
Keyword(s):  
Pituitary Gland ◽  
Leydig Cell ◽  
Testosterone Propionate ◽  
Follicle Stimulating Hormone ◽  
Inhibitory Effect ◽  
Blood Levels ◽  
Rat Pituitary ◽  
Pituitary Glands ◽  
Marked Drop ◽  
Stimulating Hormone

ABSTRACT On the basis of a study of changes in follicle stimulating hormone (FSH) levels in rat pituitary glands, obtained at various times after orchiectomy, it has been previously suggested that the Leydig cell secretions may regulate the release of FSH from the pituitary gland (Steinberger & Duckett 1966). This hypothesis was put to test in the present study. FSH levels have been determined in the pituitary gland and plasma of normal, testosterone-treated, orchiectomized, and orchiectomized testosteronetreated rats. A marked drop of pituitary FSH levels, associated with an elevation of plasma FSH levels, was observed in orchiectomized rats. Administration of testosterone to orchiectomized rats prevented the drop in pituitary FSH levels and rendered the blood levels undetectable. These results are interpreted as supporting the hypothesis that testosterone has an inhibitory effect on the release of FSH from the pituitary gland.

Regarding the validity of the endpoint response of the mouse (McKenzie) bioassay for thyrotrophin (TSH)

1981 ◽  
Vol 96 (3) ◽  
pp. 342-349 ◽  
Author(s):  
Melvin Ching
Keyword(s):  
Growth Hormone ◽  
Chemical Composition ◽  
Thyroid Hormone ◽  
Luteinizing Hormone ◽  
Pituitary Hormones ◽  
Thyroid Stimulating Hormone ◽  
Quantitative Estimates ◽  
Rat Pituitary ◽  
Stimulating Hormone

Abstract. The release of radiolabelled thyroid hormone into the circulation in low iodine fed mice has been used extensively as a bioassay for thyroid stimulating hormone (TSH). However, the specificity of several bioassays of pituitary hormones have been subject to question. Consequently, the validity of the assay endpoint for TSH in the mouse was re-evaluated with respect to the effect of luteinizing hormone (LH) whose chemical composition closely resembles that of TSH. Mice, prepared for bioassay of TSH received injections of purified LH or α or β subunits of LH. Identical doses of LH and LH subunits were quantified by LH and TSH radioimmunoassays and the results compared with those obtained by the bioassay. Microgram quantities of LH and subunits of LH elicited appreciable responses in the TSH bioassay but produced only negligible effects in the TSH radioimmunoassay. The response of the TSH bioassay of LH and α or β subunits of LH was 40–56% that obtained with LH radioimmunoassay. However, the pituitary concentrations obtained by TSH bioassay when compared with those obtained by radioimmunoassays for TSH, LH, or growth hormone (GH) paralleled closely the TSH radioimmunoassay data, although in terms of quantitative estimates, there was a 15-fold discrepancy between the TSH assays. Estimations of pituitary concentrations of LH lead to the conclusion that, at the doses normally employed, most crude rat pituitary extracts do not contain sufficient quantities of LH to alter significantly bioassayable (McKenzie) estimates of TSH.

Calretinin in the Rat Pituitary: Colocalization with Thyroid-Stimulating Hormone

1997 ◽  
Vol 65 (3) ◽  
pp. 179-188 ◽  
Author(s):  
Vincenzo Cimini ◽  
Krystyna R. Isaacs ◽  
David M. Jacobowitz
Keyword(s):  
Thyroid Stimulating Hormone ◽  
Rat Pituitary ◽  
Stimulating Hormone

ELECTROPHORETIC SEPARATION OF HORMONES ASSOCIATED WITH SECRETORY GRANULES FROM RAT ANTERIOR PITUITARY GLANDS

1970 ◽  
Vol 63 (2) ◽  
pp. 378-384 ◽  
Author(s):  
D. R. Hodges ◽  
W. H. McShan
Keyword(s):  
Growth Hormone ◽  
Anterior Pituitary ◽  
Secretory Granules ◽  
Follicle Stimulating Hormone ◽  
Pituitary Hormones ◽  
Thyroid Stimulating Hormone ◽  
Disc Electrophoresis ◽  
Hormone Activity ◽  
Pituitary Glands ◽  
Stimulating Hormone

ABSTRACT Electrophoretic analyses of rat, mouse, human and cow anterior pituitary homogenates with subsequent bioassays for hormonal activity have been reported. Comparison of the behaviour of the hormonal activities from rat anterior pituitary secretory granules and that reported for pituitary homogenates was made following disc electrophoresis on polyacrylamide gels. Bioassays of gel segments for the six anterior pituitary hormones resulted in the localization of the activities of five of the six hormones. ACTH activity was not detected. Growth hormone and prolactin were associated with the major cathodal and anodal discs respectively. Luteinizing hormone and thyroid stimulating hormone activities had similar mobilities and were located in a zone just above growth hormone. The activity was not restricted to a discrete, stainable disc in either case. Follicle stimulating hormone activity was detected in a narrow segment containing only one disc a few millimeters below growth hormone. Comparison of the mobilities of the hormones from homogenates and secretory granule extracts suggests that they have essentially similar electrophoretic characteristics at basis pH.

PURIFICATION AND PROPERTIES OF THE FOLLICLE-STIMULATING HORMONE INHIBITOR OBTAINED FROM THE URINE OF THE BONNET MONKEY

1968 ◽  
Vol 40 (2) ◽  
pp. 165-173 ◽  
Author(s):  
M. R. SAIRAM ◽  
H. G. MADHWA RAJ ◽  
N. R. MOUDGAL
Keyword(s):  
Follicle Stimulating Hormone ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Selective Extraction ◽  
Human Pituitary ◽  
Ammonium Sulphate Precipitation ◽  
Purification And Properties ◽  
Rat Pituitary ◽  
Pituitary Glands ◽  
Stimulating Hormone

SUMMARY The follicle-stimulating hormone (FSH) inhibitor in monkey urine was purified by selective extraction of the crude extract with acetate buffer, ammonium sulphate precipitation and DEAE-cellulose chromatography. The purified inhibitor was free of luteinizing hormone activity. It behaved as an apparently homogeneous protein. The inhibitor contained about 20% carbohydrate (hexoses, hexosamines, fucose and sialic acid). Thin-layer gel filtration indicated a molecular weight of about 65,000. The inhibitor was labile to heat treatment, exposure to extremes of pH and denaturing agents. The inhibitor effectively neutralized the biological activity of FSH preparations from human, monkey, horse, pig, sheep and rat pituitary glands, pregnant mare serum gonadotrophin and human pituitary urinary gonadotrophin.

Monolayer Cultures of Dispersed Cells from Bovine Anterior Pituitary: Responses to Synthetic Hypophysiotropic Peptides

1975 ◽  
Vol 53 (6) ◽  
pp. 1094-1098 ◽  
Author(s):  
G. Queen ◽  
S. Vivian ◽  
F. LaBella
Keyword(s):  
Growth Hormone ◽  
Luteinizing Hormone ◽  
Anterior Pituitary ◽  
Control Level ◽  
Thyroid Stimulating Hormone ◽  
Releasing Hormone ◽  
Cell Monolayers ◽  
Pituitary Glands ◽  
Lh Rh ◽  
Stimulating Hormone

Cells were dispersed from bovine anterior pituitary glands, by digestion with collagenase, and cultured. After 4 days the cell monolayers were incubated with fresh medium containing synthetic hypophysiotropic peptides for 2, 6, or 20 h, and hormone released into the medium was estimated by radioimmunoassay. After 2 h, thyroid releasing hormone (TRH) stimulated the release of thyroid-stimulating hormone (TSH) up to eightfold, and of prolactin (PRL) and follicle-stimulating hormone (FSH) about twofold at a minimal effective concentration of 1 ng/ml; enhanced growth hormone (GH) release was not apparent until 20 h, and release of luteinizing hormone (LH) and adrenocorticotrophic hormone (ACTH) was unaffected. Luteinizing hormone releasing hormone (LH-RH) enhanced release of LH maximally (three- to fourfold) during a 2 h incubation and was effective at 0.1 ng/ml; FSH release was significantly enhanced by about 50% above control level. Growth hormone release inhibiting hormone (GH-RIH) (somatostatin) showed significant effects only in the 20 h incubation; GH release was inhibited by 50% and release of PRL was slightly, but significantly, enhanced. Pituitary cell monolayers apparently permit maximal expression of releasing activities inherent in the hypothalamic hormones.

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