Calretinin in the Rat Pituitary: Colocalization with Thyroid-Stimulating Hormone

1997 ◽  
Vol 65 (3) ◽  
pp. 179-188 ◽  
Author(s):  
Vincenzo Cimini ◽  
Krystyna R. Isaacs ◽  
David M. Jacobowitz
Keyword(s):  
Thyroid Stimulating Hormone ◽  
Rat Pituitary ◽  
Stimulating Hormone

scholarly journals Immunofluorescence localization of glycoprotein hormones in the rat anterior pituitary gland using monoclonal antibodies and polyclonal antisera.

1982 ◽  
Vol 30 (7) ◽  
pp. 645-649 ◽  
Author(s):  
R Kofler
Keyword(s):  
Staining Technique ◽  
Thyroid Stimulating Hormone ◽  
Cell Type ◽  
Rat Pituitary ◽  
Pituitary Glands ◽  
Polyclonal Antisera ◽  
Simultaneous Identification ◽  
Mouse Antibody ◽  
Monoclonal Mouse ◽  
Stimulating Hormone

A hybridoma-derived monoclonal mouse antibody against bovine luteinizing hormone (LH) cross-reacting with rat LH and conventional polyclonal rabbit antisera against rat follicle-stimulating hormone (FSH) and thyroid-stimulating hormone (TSH) were used for the localization of the cells producing these hormones in rat pituitary glands by the immunofluorescence double staining technique. This approach enables the simultaneous identification of two different hormones on a single tissue section. It was shown that LH and FSH are produced within the same cell type, whereas TSH is synthesized by a different cell.

Regarding the validity of the endpoint response of the mouse (McKenzie) bioassay for thyrotrophin (TSH)

1981 ◽  
Vol 96 (3) ◽  
pp. 342-349 ◽  
Author(s):  
Melvin Ching
Keyword(s):  
Growth Hormone ◽  
Chemical Composition ◽  
Thyroid Hormone ◽  
Luteinizing Hormone ◽  
Pituitary Hormones ◽  
Thyroid Stimulating Hormone ◽  
Quantitative Estimates ◽  
Rat Pituitary ◽  
Stimulating Hormone

Abstract. The release of radiolabelled thyroid hormone into the circulation in low iodine fed mice has been used extensively as a bioassay for thyroid stimulating hormone (TSH). However, the specificity of several bioassays of pituitary hormones have been subject to question. Consequently, the validity of the assay endpoint for TSH in the mouse was re-evaluated with respect to the effect of luteinizing hormone (LH) whose chemical composition closely resembles that of TSH. Mice, prepared for bioassay of TSH received injections of purified LH or α or β subunits of LH. Identical doses of LH and LH subunits were quantified by LH and TSH radioimmunoassays and the results compared with those obtained by the bioassay. Microgram quantities of LH and subunits of LH elicited appreciable responses in the TSH bioassay but produced only negligible effects in the TSH radioimmunoassay. The response of the TSH bioassay of LH and α or β subunits of LH was 40–56% that obtained with LH radioimmunoassay. However, the pituitary concentrations obtained by TSH bioassay when compared with those obtained by radioimmunoassays for TSH, LH, or growth hormone (GH) paralleled closely the TSH radioimmunoassay data, although in terms of quantitative estimates, there was a 15-fold discrepancy between the TSH assays. Estimations of pituitary concentrations of LH lead to the conclusion that, at the doses normally employed, most crude rat pituitary extracts do not contain sufficient quantities of LH to alter significantly bioassayable (McKenzie) estimates of TSH.

CLINICAL EVALUATION OF THE THYROID STIMULATING HORMONE ACTIVITY IN EXOPHTHALMOS

1961 ◽  
Vol 38 (4) ◽  
pp. 577-584 ◽  
Author(s):  
Sven Erik Björkman ◽  
Torsten Denneberg ◽  
Inge Hedenskog
Keyword(s):  
Clinical Evaluation ◽  
Thyroid Stimulating Hormone ◽  
Significant Rise ◽  
Hormone Activity ◽  
Control Subjects ◽  
Long Duration ◽  
Stimulating Factor ◽  
Stimulating Hormone

ABSTRACT A method for demonstrating the presence of a thyroid stimulating factor in the blood of patients with progressive exophthalmos after thyroidectomy or after treatment with radioiodine is described. The method consists of transfusing freshly drawn blood from the patients to euthyroid recipients and subsequently following the PBI level of the recipients at regular intervals. Six exophthalmic patients tested in this manner were found to have such a factor in their circulating blood. After transfusion of their blood a significant rise in the PBI level of the recipients could be demonstrated. Two other patients, one with exophthalmos of long duration did not show this response nor did it occur after transfusion of blood from two control subjects. In one case the action of this factor was compared with that of animal thyrotrophin and found to be of the same magnitude.

Association between metabolic syndrome and thyroid stimulating hormone - A cross sectional study

2019 ◽  
Vol 9 (2) ◽  
pp. 87-90
Author(s):  
Karanam Madhuri ◽  
◽  
Rishi Kumar Venkatachalam ◽  
A Nasreen Begum ◽  
Shamsheer Khan P ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Cross Sectional Study ◽  
Thyroid Stimulating Hormone ◽  
Sectional Study ◽  
Cross Sectional ◽  
Stimulating Hormone
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