scholarly journals A new method for the study of glutaraldehyde-induced crosslinking properties in proteins with special reference to the reaction with amino groups.

1978 ◽  
Vol 26 (5) ◽  
pp. 412-414 ◽  
Author(s):  
S O Molin ◽  
H Nygren ◽  
L Dolonius
Keyword(s):  
Special Reference ◽  
High Reliability ◽  
New Method ◽  
Second Step ◽  
Amino Groups ◽  
Molar Excess ◽  
Low Protein ◽  
Modified Proteins

A new method for the study of glutaraldehyde reactions with proteins is presented. Glutaraldehyde-reacted protein is in a first step isolated and then in a second step reacted with aminohexyl groups bound to Sepharose particles. This reaction is linear at low protein concentrations and proceeds rapidly when proteins are reacted with 100-fold and 1000-fold molar excess of glutaraldehyde. This method enables the study of glutaraldehyde-induced crosslinking properties of the modified proteins as an isolated property with high reliability.

scholarly journals The histology of the cell wall, with special reference to the mode of connexion of cells. preliminary communication

1898 ◽  
Vol 62 (379-387) ◽  
pp. 100-112 ◽  
Keyword(s):  
Cell Wall ◽  
Cell Membrane ◽  
Special Reference ◽  
Original Method ◽  
New Method ◽  
Endosperm Tissue ◽  
Preliminary Communication

Since 1883 I have repeatedly endeavoured to discover some refined and generally applicable method by means of which the fine fibrillæ, or “connecting threads,” traversing the cell membrane might be identified with certainty, and the fact of their existence settled beyond dispute. I was also anxious to be in a position to investigate the development of the threads in endosperm tissue. My researches met with little encouragement until 1894, when I succeeded in finding a new method, by means of which I obtained excellent results with the young developing endosperm tissue of Tamus communis . This I have further elaborated, so that either the original method, or modifications of it, can be applied to tissues generally.

scholarly journals On the minute structure of striped muscle, with special reference to a new method of investigation, by means of ‘impressions’ stamped in collodion

1891 ◽  
Vol 49 (296-301) ◽  
pp. 287-303 ◽  
Keyword(s):  
Connective Tissue ◽  
Special Reference ◽  
New Method ◽  
Uniform Thickness ◽  
Striped Muscle

Curiously enough many of the early microscopists—Schwann for instance—recognised that the fibrils of a muscle are not simply threads of uniform thickness, like those of connective tissue: they were able to demonstrate their varicose character, even with the imperfect lenses at their command.

A New Method for Starch Gel Electrophoresis of Human Hemoglobins, with Special Reference to the Determination of Hemoglobin A2

1958 ◽  
Vol 4 (6) ◽  
pp. 484-495 ◽  
Author(s):  
C A J Goldberg
Keyword(s):  
Special Reference ◽  
Gel Electrophoresis ◽  
New Method ◽  
Resolving Power ◽  
Buffer System ◽  
Starch Gel Electrophoresis ◽  
Healthy Individuals ◽  
Starch Gel ◽  
Hemoglobin A2

Abstract A method for starch gel electrophoresis of hemoglobins is presented in which a modified Lintner starch is used for the preparation of the gel. A discontinuous buffer system of tris-EDTA-borate/barbital is used as the electrolyte medium because of its superior resolving power. Hemoglobin A2 values, obtained with this method, of healthy individuals, patients with thalassemia, and those with various anemias of nonthalassemic origin are presented.

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