scholarly journals LOCALIZATION OF α-FETOPROTEIN IN HUMAN AND RAT FETAL LIVERS AN IMMUNOHISTOCHEMICAL METHOD USING HORSERADISH PEROXIDASE

1974 ◽  
Vol 22 (6) ◽  
pp. 414-418 ◽  
Author(s):  
N. C. NAYAK ◽  
P. K. DAS ◽  
U. N. BHUYAN ◽  
ASHA MITTAL
Keyword(s):  
Connective Tissue ◽  
Horseradish Peroxidase ◽  
Immunohistochemical Method ◽  
Immunoperoxidase Technique ◽  
Serial Sections ◽  
Immunofluorescence Method ◽  
Immunohistochemical Technique

α-Fetoprotein (AFP) was successfully demonstrated in paraffin-embedded sections of human and rat fetal livers, by a multilayering immunohistochemical technique using the peroxidase-antiperoxidase system. Serial sections simultaneously subjected to immunofluorescence showed identical sites of localization in hepatocytic cytoplasm and sometimes in perivenous connective tissue. AFP-containing hepatocytes were located around efferent veins as well as randomly in the lobule. The immunoperoxidase technique has certain advantages over the immunofluorescence method which would justify the former's application in studies on the dynamics of AFP synthesis.

scholarly journals Immunuhistochemical features of the ovaries’ structure in case of fetuses with a gestational term of 21-28 weeks from mother, whose pregnancy was complicated by chronic infections of lower genital tracts

2021 ◽  
Vol 11 (5) ◽  
pp. 246-261
Author(s):  
V. O. Poliyasnyi ◽  
L. S. Kupriianova
Keyword(s):  
Connective Tissue ◽  
Main Group ◽  
Relative Volume ◽  
Immunohistochemical Method ◽  
Chronic Infections ◽  
Functional Changes ◽  
Massive Growth ◽  
Cortical Substance ◽  
Complicated Pregnancy ◽  
Clear Increase

The following article presents results of the complex research on postulation of immunohistochemical features of the ovaries’ structure in case of fetuses with a gestational term of 21-28 weeks from mothers with HILGT comparing to ones in case of fetuses from mothers with a physiological pregnancy. The materials of the research are: 25 fetuses from mothers with HILGT as well as 25 fetuses from mothers with physiological pregnancy. All fetuses had died antenatally and inranatally, as a result of violation of uteroplacental and umbilical-uterine circulation. Research methods: macroscopic, organometric, histological, immunohistochemical, morphometric, statistical. The organometric method revealed a clear decrease of main indexes of the fetuses’ ovaries from mothers with a complicated pregnancy comparing to ones in case of organs of fetuses from healthy mothers. Morphometric method revealed a clear decrease of a relative volume of cortical substance as well as a clear increase of a relative volume of brain substance in the gonads of fetuses from the main group; as well as it showed a clear decrease of an index of follicular tissue’ relative volume, likewise a clear increase of an index of interstitial component relative volume.The staining by picrofuxin by van Gieson method revealed a massive growth of the connective tissue in all strucutral components of organs of fetuses from mothers with HILGT relatively to gonads of fetuses from healthy mothers. Using of MCAT to main types of collagens allowed to reveal a violation of maturation of main types of collagens in the structure of connective tissue of fetal gonads in the main group. In the follicular component of the fetuses’ ovaries in the main group are appearing mature, cystic-artretic and growing forms of follicles. Immunohistochemical method revealed an increased number of an apoptotically altered eggs in the ovaries of fetuses from mothers with HILGT comparing to one in organs of fetuses from mothers with physiological pregnancy.  The peroxidase method revealed an increased endotheline-producing activity of the vascular component as arterial, like a venous type; likewise a clear decrease of the hormone-producing activity in the ovaries of fetuses from mothers with HILGT comparing to such indexes in fetal organs of fetuses from mothers with physiological pregnancy. The complex of changes in the ovaries of fetuses with a 21-28 weeks of gestational term, which was described, was formed under the influence of the chronic hypoxia, prolonged antigenic stimulation as well as hormonal violations in the mother-placenta-fetus system, which is a leading link of the infectious pathology’ pathogenesis. The aforementioned complex of the structural and functional changes in the ovaries of fetuses from mothers with HILGT in a gestational term of 21-28 weeks indicates violations in implementation and formation of the fetal gonads, as well as it could further lead to disorders of the germinal function in the female organism.

scholarly journals Immunohistochemical features of the structure of fetuses’ uterus with a gestational term of 21-28 weeks from mothers, whose pregnancy is complicated by chronic infection of the lower genital tract

2021 ◽  
Vol 11 (2) ◽  
pp. 230-245
Author(s):  
V. O. Polyasnyi ◽  
L. S. Kupriianova
Keyword(s):  
Connective Tissue ◽  
Genital Tract ◽  
Chronic Infection ◽  
Medical Science ◽  
Uterine Wall ◽  
Main Group ◽  
Immunohistochemical Method ◽  
Endocrine Disorders ◽  
Lower Genital Tract ◽  
Complicated Pregnancy

The relevance of the following article is prescribed by the growing number of perinatal mortality and morbidity among newborns due to the mother’s chronic infection of the lower genital tract (HILGT). It is commonly known, that HILGT contributes to the delay of the fetal development likewise is often manifested by the violation of internal organs and systems implementation. One of the most acute topic is the the fetus’ reproductive system in ther early gestation terms. However, the immunohistochemical features of the fetuses’ uterus structure with the gestational term of 21-28 weeks from mothers with HILGT on the current stage of the medical science develpment have not been described yet. The aim of the current research is to identify immunohistochemical features of the fetuses’ uterus structure with the term of gestation of 21-28 weeks from mothers, whose pregnancy is complicated by HILGT. Material of the research: 25 fetuses from mothers, whose course of pregnancy was physiological (group of comparison) as well as 25 fetuses from mothers, whose pregnancy was complicated by HILGT (main group). All fetuses had died intranatally, as a result of acute uterine placental and placental-fetal circulation. Research methods: organometric, histological, immunohistochemical, morphometric, statistical. The research results and their discussion: the organometric parameters of the fetuses’ uterus from the main group were clearily decreased in comparison to such parameters in case of the group of comparison. The decrease of the parameter of the uterine wall thickness of fetuses from mothers with the complicated pregnancy prescribes thinning of the main structural components. Namely: endometrium, myometrium and perimetrium. In case of endometrium of fetuses’ uterus a decrease of number of glands and the lack of their functional activity was noticed. It characterizes this layer as structurally immature.At the same time, in case of fetal organs of fetuses from mothers with complicated pregnancies, we could notice an increase in the number of apoptotically altered cells. Comparing to the structure of layers of uterine walls in case of fetuses from mothers with physiological pregnancy, in the uterus of fetuses from mothers with complicated pregnancy our attention is attracted by a massive growth of the connective tissue. In the myometrium it contributes to the disruption of the muscle fibers strusture likewise to its absence in some areas. By the immunohistochemical method it was possible to reveal a prevalence of the collagen of the III type in the structure of connective tissue likewise in the walls of blood vessels. It is connected primarily with disruption of the collagen’ maturation. The vascular component of the uterine wall in case of fetuses from mothers with HILGT differs from ones in case of fetuses from mothers with physiological pregnancy. This difference is grounded on the increase of endothelin-producing activity by vessels of both arterial and venous types. By processing preparations by monoclonal antibodies to estrogen, it has been postulated, that the intensity of the reaction as well as number of cells in the fetuses’ organs of the main group are clearily decreased comparing to ones in cases of fetuses from the group of comparison. The disorders of the fetuses’ uterus in case of fetuses from mothers with HILGT, that were provided in the current article, are prescribed by chronic hypoxia, endocrine disorders as well as influences of the infectious agents, that are taking place in case of such pathology. The characteristic features of the fetuses’ uterus structure in case of fetuses from mothers, whose pregnancy is complicated by HILGT, indicate gross violation in the organ’s implementation on the early stages of the fetal organogenesis, likewise they could be a leading link of pathogenesis of primary infertility’ development in the future ontogenesis.

An Improved Immunoperoxidase Technique Using Horseradish Peroxidase Avidin D

1989 ◽  
Vol 20 (2) ◽  
pp. 109-112 ◽  
Author(s):  
Hari M. Sharma ◽  
Ellen M. Kauffman ◽  
Christine M. Conrad
Keyword(s):  
Horseradish Peroxidase ◽  
Immunoperoxidase Technique

scholarly journals Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus

1977 ◽  
Vol 5 (6) ◽  
pp. 629-634
Author(s):  
H Schmitz ◽  
H W Doerr ◽  
D Kampa ◽  
A Vogt
Keyword(s):  
Alkaline Phosphatase ◽  
Horseradish Peroxidase ◽  
Enzyme Immunoassay ◽  
Indirect Immunofluorescence ◽  
Solid Phase ◽  
Immunoglobulin M ◽  
Nuclear Antigen ◽  
Immunofluorescence Method ◽  
Infected Cells

A sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin M antibodies to cytomegalovirus is described. The results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. Horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. Nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei of cytomegalovirus-infected cells.

scholarly journals Immunohistochemical localization of acetylcholine receptors at human endplates using a monoclonal antibody.

1987 ◽  
Vol 35 (5) ◽  
pp. 613-617 ◽  
Author(s):  
L M Smit ◽  
H Veldman ◽  
F G Jennekens
Keyword(s):  
Monoclonal Antibody ◽  
Acetylcholine Receptors ◽  
Immunohistochemical Localization ◽  
Immunohistochemical Method ◽  
Electron Microscopic Level ◽  
Immunoperoxidase Technique ◽  
Electron Microscopic ◽  
Fixed Tissue ◽  
Alpha Bungarotoxin ◽  
Diagnostic Investigations

We describe a simple indirect immunohistochemical method for localization of acetylcholine receptors (AChR) in motor endplates at the light and electron microscopic level. This method involves the use of a monoclonal antibody directed against the main immunogenic region (MIR) of AChRs and is applicable to periodate-lysine-paraformaldehyde (PLP)-fixed tissue. We discuss the advantages of this method, as compared with the alpha-bungarotoxin-immunoperoxidase technique, and stress its value for diagnostic investigations of motor point biopsies from patients with neuromuscular transmission disorders.

Intrachondral Microvasculature in the Human Fetal Talus

1997 ◽  
Vol 18 (6) ◽  
pp. 335-338 ◽  
Author(s):  
Xiaoguang Cheng ◽  
Yunzhao Wang ◽  
Hui Qu
Keyword(s):  
Connective Tissue ◽  
Blood Vessels ◽  
Ossification Center ◽  
Serial Sections ◽  
Sinus Tarsi ◽  
Cartilage Canals ◽  
Tarsal Canal ◽  
Primary Ossification Center ◽  
Vascular Injection ◽  
Adjacent Cartilage

The intrachondral microvasculature of the growing talus of human was studied in 16 fetuses aged from 15 to 44 weeks of gestation, using interrupted serial sections and vascular injection of ink. The cartilage model of the talus was shown to be well vascularized throughout by cartilage canals. The cartilage canal contained blood vessels and connective tissue, with vessels originating from the perichondrial vessels. They were covered by a thick connective tissue wall that was continuous with the perichondrium. The functions of the cartilage canals were mainly to nourish the large masses of cartilage and to supply osteogenic tissue, which initiates the primary ossification center. As in the adult, the fetal talus was supplied with four to five main branches originating from the sinus tarsi and the tarsal canal; there were no anastomoses between the vessels of the adjacent cartilage canals and between the branches within the cartilage canal. This type of microvasculature is vulnerable to injury and, if impaired, may cause serious complications.

scholarly journals Spicules and skeletons: mantle musculature of two species of dorid nudibranchs (Gastropoda: Nudibranchia: Doridina)

2020 ◽  
Vol 98 (12) ◽  
pp. 777-786
Author(s):  
B.K. Penney ◽  
J.D. Sigwart ◽  
D.Y. Parkinson
Keyword(s):  
Computed Tomography ◽  
Connective Tissue ◽  
Complex Networks ◽  
Serial Sections ◽  
Micro Computed Tomography ◽  
Digital Models ◽  
Dorsal Tubercle

Molluscs often possess complex calcified elements in addition to the shell, but how these elements function and relate to other tissues is often poorly understood. Dorid nudibranchs typically possess innumerable calcareous spicules arranged in complex networks. To describe how these spicules interact with muscles and connective tissue, we reconstructed tomographic digital models using serial sections and synchrotron micro-computed tomography. In two species with dramatically different spicule network morphologies, musculature was divided into a dorsal layer of crossed fibres, a ventral layer of branching radial fibres, and scattered dorsoventral fibres in between. These two species differed in the size of their dorsal tubercles, which was reflected in the organization of dorsal musculature, and in the amount and organization of connective tissue. In Platydoris sanguinea Bergh, 1905, dense mats of spicules sandwiched a layer of connective tissue with fewer spicules and muscle insertions only onto the ventral spicules. In Cadlina luteomarginata MacFarland, 1966, thick tracts of spicules are surrounded by a sheath of connective tissue. Muscles surround and insert into the dorsal tubercle spicule layer. Thus, both species appear to use the spicule network for muscle antagonism and transfer of motion, but the different arrangement of elements suggests that they use this skeleton in quite different ways.

scholarly journals Growth hormone releasing factor (GRF) immunoreactivity in human and rat gastrointestinal tract and pancreas.

1984 ◽  
Vol 32 (11) ◽  
pp. 1139-1144 ◽  
Author(s):  
F T Bosman ◽  
C Van Assche ◽  
A C Nieuwenhuyzen Kruseman ◽  
S Jackson ◽  
P J Lowry
Keyword(s):  
Growth Hormone ◽  
Pancreatic Islets ◽  
Endocrine Tumor ◽  
Cross Reactivity ◽  
Gastric Antrum ◽  
Immunoperoxidase Technique ◽  
Serial Sections ◽  
Growth Hormone Releasing Factor ◽  
Indirect Immunoperoxidase ◽  
Synthetic Growth

Rabbit antisera were raised against a synthetic growth hormone releasing factor, which was originally isolated from a human pancreatic endocrine tumor (hpGRF-44). The antisera obtained showed no significant cross-reactivity with a variety of neurohormonal peptides. In addition to its occurrence in the human, but not in the rat, hypothalamus, hpGRF-44-like immunoreactivity was identified in human gastric antrum and human as well as rat pancreatic islets, using an indirect immunoperoxidase technique. Staining of serial sections and double staining revealed that in the gastric antrum the immunoreactivity was largely confined to gastrin (G) cells, whereas in pancreatic islets polypeptide (pp) cells were reactive. The physiological significance of these findings remains to be established.

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