scholarly journals HUMAN LEUKEMIC CELLS CYTOCHEMICAL STUDIES ON ACIDIC NUCLEAR PROTEINS

1974 ◽  
Vol 22 (1) ◽  
pp. 40-45 ◽  
Author(s):  
L. S. DESAI ◽  
G. E. FOLEY
Keyword(s):  
Toluidine Blue ◽  
Diagnostic Category ◽  
Distribution Patterns ◽  
Dry Mass ◽  
Nuclear Proteins ◽  
Leukemic Cells ◽  
Control Mechanisms ◽  
Acidic Proteins ◽  
Human Leukemic Cells ◽  
Individual Human

Utilizing staining with alkaline fast green (pH 8.1) and toluidine blue O (pH 9.0) stains, the nuclear basic and acidic protein content of individual human lymphocytic cells has been quantitated cytochemically with high resolution, rapid scanning instruments. There were distinct differences in the distribution patterns of the acidic and basic proteins in these human lymphocytic cells. Nucleoli were densely stained by toluidine blue O, confirming the presence of acidic proteins. The acidic nuclear proteins:DNA and basic nuclear proteins:DNA ratios are consistent and quantitatively similar, irrespective of the diagnostic category from which the cells derived and there was a definite correlation between nuclear proteins and DNA content. However, the acidic nuclear proteins represented a larger proportion of the total dry mass of the cell than did the basic nuclear proteins, an observation which may prove to be of interest with respect to control mechanisms.

scholarly journals EVALUATION OF A CYTOCENTRIFUGE TECHNIQUE EFFECTS ON QUANTITATIVE DETERMINATION OF FEULGEN-DEOXYRIBONUCLEIC ACID AND TOTAL DRY MASS OF HUMAN LEUKEMIC CELLS

1972 ◽  
Vol 20 (9) ◽  
pp. 703-709 ◽  
Author(s):  
L. E. DE BAULT
Keyword(s):  
Deoxyribonucleic Acid ◽  
Lymphoblastic Leukemia ◽  
Dry Mass ◽  
Suspension Cultures ◽  
Leukemic Cells ◽  
Specimen Preparation ◽  
Cell Parameters ◽  
Cytochemical Analysis ◽  
Dna Contents ◽  
Human Leukemic Cells

The cytocentrifuge has been used as a tool to obtain preparations from suspension cultures suitable for quantitative cytochemical analysis. Three continuous suspension cultures of human lymphocytic cells (CCRF-CEM, acute lymphoblastic leukemia; CCRF-RKB, infectious mononucleosis; and EB-3, Burkitt's lymphoma) and a suspension of mouse inguinal lymph node lymphocytes were examined for quantitative differential effects on cellular dry mass and Feulgen-deoxyribonucleic acid (DNA) contents caused by cytocentrifugation. When using a cytocentrifuge technique which results in a dry preparation, a quantitative loss was observed for cellular dry mass but not for Feulgen-DNA. However, if a medium which prevented bursting, swelling or loss of cellular constituents during cytocentrifugation was used and if the preparation was maintained in the wet state (no air-drying), acceptable preparations for both dry mass and Feulgen-DNA quantitation can be obtained. These and other observations on centrifugal force, centrifuge time and suspending medium emphasize the importance of specimen preparation when quantitating cell parameters that may themselves be altered by the method of preparation.

Human leukemic cells: Cytochemical studies on nuclear proteins

Chromosoma ◽  
1972 ◽  
Vol 38 (3) ◽  
pp. 329-340 ◽  
Author(s):  
L. S. Desai ◽  
L. E. De Bault ◽  
G. Morrissey ◽  
G. E. Foley
Keyword(s):  
Nuclear Proteins ◽  
Leukemic Cells ◽  
Human Leukemic Cells

A novel mechanism for imatinib mesylate–induced cell death of BCR-ABL–positive human leukemic cells: caspase-independent, necrosis-like programmed cell death mediated by serine protease activity

Blood ◽  
2004 ◽  
Vol 103 (6) ◽  
pp. 2299-2307 ◽  
Author(s):  
Masayuki Okada ◽  
Souichi Adachi ◽  
Tsuyoshi Imai ◽  
Ken-ichiro Watanabe ◽  
Shin-ya Toyokuni ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Serine Protease ◽  
Imatinib Mesylate ◽  
Protease Activity ◽  
Kinase Inhibitor ◽  
Leukemic Cells ◽  
Endonuclease G ◽  
Serine Protease Activity ◽  
Human Leukemic Cells

Abstract Caspase-independent programmed cell death can exhibit either an apoptosis-like or a necrosis-like morphology. The ABL kinase inhibitor, imatinib mesylate, has been reported to induce apoptosis of BCR-ABL–positive cells in a caspase-dependent fashion. We investigated whether caspases alone were the mediators of imatinib mesylate–induced cell death. In contrast to previous reports, we found that a broad caspase inhibitor, zVAD-fmk, failed to prevent the death of imatinib mesylate–treated BCR-ABL–positive human leukemic cells. Moreover, zVAD-fmk–preincubated, imatinib mesylate–treated cells exhibited a necrosis-like morphology characterized by cellular pyknosis, cytoplasmic vacuolization, and the absence of nuclear signs of apoptosis. These cells manifested a loss of the mitochondrial transmembrane potential, indicating the mitochondrial involvement in this caspase-independent necrosis. We excluded the participation of several mitochondrial factors possibly involved in caspase-independent cell death such as apoptosis-inducing factor, endonuclease G, and reactive oxygen species. However, we observed the mitochondrial release of the serine protease Omi/HtrA2 into the cytosol of the cells treated with imatinib mesylate or zVAD-fmk plus imatinib mesylate. Furthermore, serine protease inhibitors prevented the caspase-independent necrosis. Taken together, our results suggest that imatinib mesylate induces a caspase-independent, necrosis-like programmed cell death mediated by the serine protease activity of Omi/HtrA2.

The impact of lithium on human leukemic cells

1991 ◽  
Vol 15 (8) ◽  
pp. 766 ◽  
Author(s):  
Ronald D. Barr ◽  
Delsworth Harnish
Keyword(s):  
Leukemic Cells ◽  
Human Leukemic Cells ◽  
The Impact

Altered catalytic activity of and DNA cleavage by DNA topoisomerase II from human leukemic cells selected for resistance to VM-26

Biochemistry ◽  
1988 ◽  
Vol 27 (24) ◽  
pp. 8861-8869 ◽  
Author(s):  
Mary K. Danks ◽  
Carla A. Schmidt ◽  
Margaret C. Cirtain ◽  
D. Parker Suttle ◽  
William T. Beck
Keyword(s):  
Catalytic Activity ◽  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Leukemic Cells ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Human Leukemic Cells

Immunological Markers of Human Leukemic Cells of the B Lineage

1983 ◽  
pp. 29-43
Author(s):  
M. Seligmann ◽  
L. B. Vogler ◽  
P. Guglielmi ◽  
J. C. Brouet ◽  
J. L. Preud’homme
Keyword(s):  
Leukemic Cells ◽  
Immunological Markers ◽  
B Lineage ◽  
Human Leukemic Cells

Effect of 4-hydroxynonenal on gene expression and differentiation of HL-60 human leukemic cells

1991 ◽  
Vol 27 ◽  
pp. S65 ◽  
Author(s):  
G. Barrera ◽  
R. Muraca ◽  
C. Di Mauro ◽  
V.M. Fazio ◽  
M.U. Dianzani
Keyword(s):  
Gene Expression ◽  
Leukemic Cells ◽  
Human Leukemic Cells
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