Altered catalytic activity of and DNA cleavage by DNA topoisomerase II from human leukemic cells selected for resistance to VM-26

Biochemistry ◽  
1988 ◽  
Vol 27 (24) ◽  
pp. 8861-8869 ◽  
Author(s):  
Mary K. Danks ◽  
Carla A. Schmidt ◽  
Margaret C. Cirtain ◽  
D. Parker Suttle ◽  
William T. Beck
Keyword(s):  
Catalytic Activity ◽  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Leukemic Cells ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Human Leukemic Cells

scholarly journals Coupling between ATP Binding and DNA Cleavage by DNA Topoisomerase II

2008 ◽  
Vol 283 (25) ◽  
pp. 17463-17476 ◽  
Author(s):  
Felix Mueller-Planitz ◽  
Daniel Herschlag
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Atp Binding ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase

scholarly journals Cytotoxicity and inhibitory properties against topoisomerase II of doxorubicin and its formamidine derivatives.

2009 ◽  
Vol 56 (1) ◽  
Author(s):  
Krzysztof Kik ◽  
Kazimierz Studzian ◽  
Małgorzata Wasowska-Łukawska ◽  
Irena Oszczapowicz ◽  
Leszek Szmigiero
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Colorimetric Assay ◽  
Cellular Level ◽  
Cytotoxic Action ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Free System ◽  
Cell Free System ◽  
Pbr322 Dna

This work was undertaken to compare cytotoxicity, DNA damaging properties and effect on DNA cleavage by topoisomerase II of the anthracycline drug doxorubicin (DOX) and its two derivatives with a formamidino group containing a cyclic amine moiety such as morpholine (DOXM) or hexamethyleneimine (DOXH). The tetrazolium dye colorimetric assay was used to determine the cytotoxic activity of anthracyclines toward L1210 leukemia cells. DNA damage was measured by alkaline elution technique. The effect of anthracyclines on DNA cleavage was studied in a cell-free system containing supercoiled pBR322 DNA and purified human topoisomerase II. The cytotoxicity data and the results of studies on the mechanism of DNA break formation by anthracyclines at the cellular level and in the cell-free system showed that the presence of the formamidino group in the doxorubicin molecule reduced its ability to stimulate DNA cleavage by DNA topoisomerase II. DNA topoisomerase II is not a primary cellular target for DOXM or DOXH. An advantageous feature of formamidinoanthracyclines is their mechanism of cytotoxic action which is not related to the inhibition of DNA topoisomerase II. Therefore this class of anthracyclines seems to be a good source for selection of an anticancer drug directed toward cancer cells with the developed multidrug resistance attributed to the presence of altered DNA topoisomerase II.

Amsacrine-promoted DNA cleavage site determinants for the two human DNA topoisomerase II isoforms α and β

1996 ◽  
Vol 52 (11) ◽  
pp. 1675-1685 ◽  
Author(s):  
Katherine L. Marsh ◽  
Elaine Willmore ◽  
Stella Tinelli ◽  
Mariagrazia Cornarotti ◽  
Emma L. Meczes ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Cleavage Site ◽  
Topoisomerase Ii ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Human Dna

Reduced DNA topoisomerase II activity and drug-stimulated DNA cleavage in 9-hydroxyellipticine resistant cells

1988 ◽  
Vol 37 (11) ◽  
pp. 2145-2149 ◽  
Author(s):  
Jean-Yves Charcosset ◽  
Jean-Marie Saucier ◽  
Alain Jacquemin-Sablon
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Resistant Cells
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