scholarly journals HISTOCHEMICAL DEMONSTRATION OF SUCCINIC DEHYDROGENASE ACTIVITY IN TISSUE SECTIONS BY A MODIFIED TECHNIQUE

1954 ◽  
Vol 2 (2) ◽  
pp. 110-114 ◽  
Author(s):  
CHARLES G. ROSA ◽  
JOSEPH T. VELARDO
Keyword(s):  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Histochemical Demonstration ◽  
Succinic Dehydrogenase Activity ◽  
Modified Technique ◽  
Tissue Sections

scholarly journals THE DISTRIBUTION OF HISTOCHEMICALLY DEMONSTRABLE SUCCINIC DEHYDROGENASE AND OF MITOCHONDRIA IN TONGUE AND SKELETAL MUSCLES

1955 ◽  
Vol 1 (6) ◽  
pp. 483-488 ◽  
Author(s):  
M. Wachstein ◽  
E. Meisel
Keyword(s):  
Abdominal Wall ◽  
Dehydrogenase Activity ◽  
Skeletal Muscles ◽  
Succinic Dehydrogenase ◽  
Lower Extremities ◽  
Close Similarity ◽  
Succinic Dehydrogenase Activity ◽  
Tissue Sections ◽  
Marked Activity

Various skeletal muscles show considerable variations in histochemically demonstrable succinic dehydrogenase activity. In muscles from the lower extremities, the back, and the abdominal wall, only some fibers reveal evidence of enzymatic staining. In the diaphragm and masseter more fibers react positively. In the tongue, however, all fibers show marked activity comparable to that found regularly in the heart. There is a close similarity in the distribution of histochemically demonstrable succinic dehydrogenase and stainable mitochondria in tissue sections.

scholarly journals UBIQUINONE AND PHOSPHOLIPIDS AS LIMITING FACTORS IN THE HISTOCHEMICAL DEMONSTRATION OF SUCCINIC DEHYDROGENASE ACTIVITY

1967 ◽  
Vol 15 (2) ◽  
pp. 79-82 ◽  
Author(s):  
MOSHE WOLMAN ◽  
JOSE JAIME BUBIS
Keyword(s):  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Histochemical Demonstration ◽  
Mouse Kidney ◽  
Limiting Factors ◽  
Succinic Dehydrogenase Activity ◽  
Similar Treatment ◽  
Kidney Liver ◽  
Cryostat Sections ◽  
Long Storage

Deposition of ubiquinone and preferably of a crude phospholipid mixture and ubiquinone on cryostat sections of mouse kidney, liver and heart increased the intensity of staining for succinic dehydrogenase. A similar treatment partly restored the activity of this enzyme which was reduced by exposure to 60°C, long storage at 37°C, oxidation and treatment with lipid solvents. The restoration was present only in the case of mild noxious treatments. It is suggested that removal or structural alteration of ubiquinone and phospholipids may limit the rate of dehydrogenase activity; more drastic treatments affect the enzyme itself so that these factors cannot exert any restorative activity.

Sign in / Sign up

Export Citation Format

Share Document