scholarly journals UBIQUINONE AND PHOSPHOLIPIDS AS LIMITING FACTORS IN THE HISTOCHEMICAL DEMONSTRATION OF SUCCINIC DEHYDROGENASE ACTIVITY

1967 ◽  
Vol 15 (2) ◽  
pp. 79-82 ◽  
Author(s):  
MOSHE WOLMAN ◽  
JOSE JAIME BUBIS
Keyword(s):  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Histochemical Demonstration ◽  
Mouse Kidney ◽  
Limiting Factors ◽  
Succinic Dehydrogenase Activity ◽  
Similar Treatment ◽  
Kidney Liver ◽  
Cryostat Sections ◽  
Long Storage

Deposition of ubiquinone and preferably of a crude phospholipid mixture and ubiquinone on cryostat sections of mouse kidney, liver and heart increased the intensity of staining for succinic dehydrogenase. A similar treatment partly restored the activity of this enzyme which was reduced by exposure to 60°C, long storage at 37°C, oxidation and treatment with lipid solvents. The restoration was present only in the case of mild noxious treatments. It is suggested that removal or structural alteration of ubiquinone and phospholipids may limit the rate of dehydrogenase activity; more drastic treatments affect the enzyme itself so that these factors cannot exert any restorative activity.

Histochemistry of Hymenolepis microstoma (Cestoda: Hymenolepididae)

Parasitology ◽  
1966 ◽  
Vol 56 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Burton J. Bogitsh ◽  
David A. Nunnally
Keyword(s):  
Enzyme Activity ◽  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Anterior Region ◽  
Succinic Dehydrogenase Activity ◽  
Blue Tetrazolium ◽  
High Concentrations ◽  
Intense Activity ◽  
Cryostat Sections ◽  
Hymenolepis Microstoma

Reduction of neotetrazolium in the presence of succinate is used as a quantitative assay for succinic dehydrogenase activity in homogenates of Hymenolepis microstoma, and nitro blue tetrazolium and thiazolyl blue tetrazolium-cobalt are employed with cryostat sections for its histochemical localization in adults and cysticercoids. The highest concentrations of the enzyme occur in the anterior region of the worm (scolex, neck, and immature proglottids), lesser amounts in the region of mature and early gravid segments, and least amounts in the gravid region of the strobila. Primary sites of succinic dehydrogenase activity are the mitochondrial layer of the cuticle and the cells of the subcuticle. In the anterior region the entire parenchyma shows uniformly high concentrations of enzyme activity. This reaction decreases posteriorly until, in the gravid region, only the subcuticle and the mitochondrial layer of the cuticle display activity. Intense activity was also noted in the layer just under the shells of the eggs. The ovary and immature testes show high enzyme concentrations; however, in the testes, the concentration diminishes as the organs mature. In the scolex, the rostellum and the muscles of the suckers show higher enzyme activity than the surrounding tissues. The scolex, inner membrane, and tail of the cysticercoid show uniformly high concentrations of formazan.

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