Identifying the functional part of heparin-binding protein (HBP) as a monocyte stimulator and the novel role of monocytes as HBP producers

2010 ◽  
Vol 17 (1) ◽  
pp. 60-69 ◽  
Author(s):  
Morten Schou ◽  
René Djurup ◽  
Kjeld Norris ◽  
Hans Flodgaard
Keyword(s):  
Binding Protein ◽  
The Novel ◽  
Heparin Binding ◽  
Heparin Binding Protein ◽  
Functional Part

scholarly journals Heparin-Binding Protein 17/Fibroblast Growth Factor-Binding Protein-1 Knockout Inhibits Proliferation and Induces Differentiation of Squamous Cell Carcinoma Cells

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2684
Author(s):  
Tomoaki Shintani ◽  
Mirai Higaki ◽  
Tetsuji Okamoto
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Squamous Cell ◽  
Binding Protein ◽  
Squamous Cell Carcinomas ◽  
Fibroblast Growth ◽  
Heparin Binding ◽  
Heparin Binding Protein

Heparin-binding protein 17/fibroblast growth factor-binding protein-1 (HBp17/FGFBP-1) has been observed to induce the tumorigenic potential of epithelial cells and is highly expressed in oral cancer cell lines and tissues. It is also recognized as a pro-angiogenic molecule because of its interaction with fibroblast growth factor (FGF)-2. In this study, we examined the functional role of HBp17/FGFBP-1 in A431 and HO-1-N-1 cells. Originally, HBp17/FGFBP-1 was purified from A431 cell-conditioned media based on its capacity to bind to FGF-1 and FGF-2. We isolated and established HBp17/FGFBP-1-knockout (KO)-A431 and KO-HO-1-N-1 cell lines using the clusters of regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) gene editing technology. The amount of FGF-2 secreted into conditioned medium decreased for A431-HBp17-KO and HO-1-N-1-HBp17-KO cells compared to their WT counterparts. Functional assessment showed that HBp17/FGFBP-1 KO inhibited cell proliferation, colony formation, and cell motility in vitro. It also inhibited tumor growth in vivo compared to controls, which confirmed the significant difference in growth in vitro between HBp17-KO cells and wild-type (WT) cells, indicating that HBp17/FGFBP-1 is a potent therapeutic target in squamous cell carcinomas (SCC) and oral squamous cell carcinomas (OSCC). In addition, complementary DNA/protein expression analysis followed by Gene Ontology and protein–protein interaction (PPI) analysis using the Database for Visualization and Integrated Discovery and Search Tool for the Retrieval of Interacting Genes/Proteins showed that both gene and protein expression related to epidermal development, cornification, and keratinization were upregulated in A431-HBp17-KO and HO-1-N-1-KO cells. This is the first discovery of a novel role of HBp17/FGFBP-1 that regulates SCC and OSCC cell differentiation.

The role of β2 integrin associated heparin-binding protein release in ARDS

Life Sciences ◽  
2018 ◽  
Vol 203 ◽  
pp. 92-98 ◽  
Author(s):  
Yang Liu ◽  
Shaolin Ma ◽  
Xuebin Wang ◽  
Yajing Feng ◽  
Shouqin Zhang ◽  
...  
Keyword(s):  
Binding Protein ◽  
Protein Release ◽  
Heparin Binding ◽  
Β2 Integrin ◽  
Heparin Binding Protein

The heparin-binding protein interactome in pancreatic diseases

Pancreatology ◽  
2013 ◽  
Vol 13 (6) ◽  
pp. 598-604 ◽  
Author(s):  
Q.M. Nunes ◽  
V. Mournetas ◽  
B. Lane ◽  
R. Sutton ◽  
D.G. Fernig ◽  
...  
Keyword(s):  
Binding Protein ◽  
Heparin Binding ◽  
Pancreatic Diseases ◽  
Heparin Binding Protein ◽  
Protein Interactome

Heparin-binding protein (HBP/CAP37): A missing link in neutrophil-evoked alteration of vascular permeability

2001 ◽  
Vol 7 (10) ◽  
pp. 1123-1127 ◽  
Author(s):  
Narinder Gautam ◽  
A. Maria Olofsson ◽  
Heiko Herwald ◽  
Lars F. Iversen ◽  
Evy Lundgren-Åkerlund ◽  
...  
Keyword(s):  
Vascular Permeability ◽  
Binding Protein ◽  
Heparin Binding ◽  
Missing Link ◽  
Heparin Binding Protein

Human C21orf63 is a Heparin-binding Protein

2009 ◽  
Vol 146 (3) ◽  
pp. 369-373 ◽  
Author(s):  
Kanae Mitsunaga ◽  
Jun Harada-Itadani ◽  
Toshihide Shikanai ◽  
Hiroaki Tateno ◽  
Yuzuru Ikehara ◽  
...  
Keyword(s):  
Binding Protein ◽  
Heparin Binding ◽  
Heparin Binding Protein

Heparin Binding Protein for The Early Diagnosis and Prognosis Of Sepsis In The Emergency Department

Shock ◽  
2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Konstantinos Katsaros ◽  
Georgios Renieris ◽  
Asimina Safarika ◽  
Evangelia-Maria Adami ◽  
Theologia Gkavogianni ◽  
...  
Keyword(s):  
Emergency Department ◽  
Early Diagnosis ◽  
Binding Protein ◽  
Heparin Binding ◽  
Diagnosis And Prognosis ◽  
Heparin Binding Protein
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