scholarly journals CYTOLOGICAL LOCALIZATION OF NORADRENALINE, MONOAMINE OXIDASE AND ACETYLCHOLINESTERASE IN SALIVARY GLANDS OF DOG

1966 ◽  
Vol 14 (6) ◽  
pp. 483-490 ◽  
Author(s):  
MOTOHATSU FUJIWARA ◽  
CHIKAKO TANAKA ◽  
HIROSHI HIKOSAKA ◽  
TADAO OKEGAWA

The cytological localization of noradrenaline, monoamine oxidase and acetylcholinesterase was examined in the salivary glands of the dog. The noradrenaline-fluorescent nerve fibers surrounded the secretory acini of the submaxillary, sublingual and parotid glands, but the density in the sublingual gland was much less than in the other two glands. Noradrenaline fluorescence was not seen in the cytoplasms of the acinar cells nor in the vicinity of the excretory ducts. Intense fluorescence was present outside the smooth muscle layer in various sized arteries. The distribution of monoamine oxidase activity was different from that of noradrenaline fluoresence. All the cells of the secretory acini and the excretory ducts were evenly stained. The distribution of fibers showing acetylcholinesterase activity was different from that of noradrenaline-fluorescent fibers only in that the former fibers were present around the excretory ducts as well. The composition of secretory cells in the submaxillary and sublingual glands is different in dogs and rats. However, the cytological localization of noradrenaline, monoamine oxidase and acetylcholinesterase activities in the salivary glands of the dog was essentially similar to those of the rat. It was concluded, therefore, that the autonomic nerve supply to the salivary glands does not correlate with the type of secretory cells.

Author(s):  
John L. Beggs ◽  
Peter C. Johnson ◽  
Astrid G. Olafsen ◽  
C. Jane Watkins

The blood supply (vasa nervorum) to peripheral nerves is composed of an interconnected dual circulation. The endoneurium of nerve fascicles is maintained by the intrinsic circulation which is composed of microvessels primarily of capillary caliber. Transperineurial arterioles link the intrinsic circulation with the extrinsic arterial supply located in the epineurium. Blood flow in the vasa nervorum is neurogenically influenced (1,2). Although a recent hypothesis proposes that endoneurial blood flow is controlled by the action of autonomic nerve fibers associated with epineurial arterioles (2), our recent studies (3) show that in addition to epineurial arterioles other segments of the vasa nervorum are also innervated. In this study, we examine blood vessels of the endoneurium for possible innervation.


1974 ◽  
Vol 31 (03) ◽  
pp. 403-414 ◽  
Author(s):  
Terence Cartwright

SummaryA method is described for the extraction with buffers of near physiological pH of a plasminogen activator from porcine salivary glands. Substantial purification of the activator was achieved although this was to some extent complicated by concomitant extraction of nucleic acid from the glands. Preliminary characterization experiments using specific inhibitors suggested that the activator functioned by a similar mechanism to that proposed for urokinase, but with some important kinetic differences in two-stage assay systems. The lack of reactivity of the pig gland enzyme in these systems might be related to the tendency to protein-protein interactions observed with this material.


1941 ◽  
Vol 24 (4) ◽  
pp. 483-504 ◽  
Author(s):  
G. H. Parker

1. When appropriate chromatic nerves are cut caudal bands, cephalic areas, and the pelvic fins of the catfish Ameiurus darken. In pale fishes all these areas will sooner or later blanch. By recutting their nerves all such blanched areas will darken again. 2. These observations show that the darkening of caudal bands, areas, and fins on cutting their nerves is not due to paralysis (Brücke), to the obstruction of central influences such as inhibition (Zoond and Eyre), nor to vasomotor disturbances (Hogben), but to activities emanating from the cut itself. 3. The chief agents concerned with the color changes in Ameiurus are three: intermedin from the pituitary gland, acetylcholine from the dispersing nerves (cholinergic fibers), and adrenalin from the concentrating nerves (adrenergic fibers). The first two darken the fish; the third blanches it. In darkening the dispersing nerves appear to initiate the process and to be followed and substantially supplemented by intermedin. 4. Caudal bands blanch by lateral invasion, cephalic areas by lateral invasion and internal disintegration, and pelvic fins by a uniform process of general loss of tint equivalent to internal disintegration. 5. Adrenalin may be carried in such an oil as olive oil and may therefore act as a lipohumor; it is soluble in water and hence may act as a hydrohumor. In lateral invasion (caudal bands, cephalic areas) it probably acts as a lipohumor and in internal disintegration (cephalic areas, pelvic fins) it probably plays the part of a hydrohumor. 6. The duration of the activity of dispersing nerves after they had been cut was tested by means of the oscillograph, by anesthetizing blocks, and by cold-blocks. The nerves of Ameiurus proved to be unsatisfactory for oscillograph tests. An anesthetizing block, magnesium sulfate, is only partly satisfactory. A cold-block, 0°C., is successful to a limited degree. 7. By means of a cold-block it can be shown that dispersing autonomic nerve fibers in Ameiurus can continue in activity for at least 6½ hours. It is not known how much longer they may remain active. So far as the duration of their activity is concerned dispersing nerve fibers in this fish are unlike other types of nerve fibers usually studied.


1964 ◽  
Vol 11 (4) ◽  
pp. 283-290 ◽  
Author(s):  
TAKASHI KOBAYASHI ◽  
TAKURO KOBAYASHI ◽  
JUNZO KATO ◽  
HIROSHI MINAGUCHI

1982 ◽  
Vol 30 (10) ◽  
pp. 3803-3806 ◽  
Author(s):  
MASATOSHI YAMAGUCHI ◽  
HITOSHI NOHTA ◽  
YOSUKE OHKURA

1980 ◽  
Vol 3 (3) ◽  
pp. 273-279 ◽  
Author(s):  
Christopher John Fowler ◽  
Lars von Knorring ◽  
Lars Oreland

Cephalalgia ◽  
1991 ◽  
Vol 11 (11_suppl) ◽  
pp. 254-255 ◽  
Author(s):  
Aron D. Mosnaim ◽  
Shankar Huprikar ◽  
Marion E. Wolf ◽  
Frederick Freitag ◽  
Seymour Diamond

1984 ◽  
Vol 11 (3) ◽  
pp. 211-221 ◽  
Author(s):  
Robert J. Hitzemann ◽  
Frederick Sautter ◽  
Barbara Hitzemann ◽  
Jack Hirschowitz ◽  
David L. Garver

The Lancet ◽  
1977 ◽  
Vol 310 (8052) ◽  
pp. 1325-1327 ◽  
Author(s):  
JohnL. Sullivan ◽  
Allan Maltbie ◽  
JesseO. Cavenar ◽  
Charles Stanfield

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