scholarly journals DUAL STAINING OF MAST CELL CYTOPLASMIC CONSTITUENTS BY ALCIAN BLUE AND SAFRANIN

1962 ◽  
Vol 10 (4) ◽  
pp. 503-503 ◽  
Author(s):  
W. CURTIS WORTHINGTON ◽  
NAN C. BAILEY
Keyword(s):  
Mast Cell ◽  
Alcian Blue ◽  
Dual Staining

Mast Cell Heterogeneity in Human Lung Tissue

1989 ◽  
Vol 77 (3) ◽  
pp. 297-304 ◽  
Author(s):  
F. J. Van Overveld ◽  
L. A. M. J. Houben ◽  
F. E. M. Schmitz du Moulin ◽  
P. L. B. Bruijnzeel ◽  
J. A. M. Raaijmakers ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Lung Tissue ◽  
Human Lung ◽  
Immunoglobulin E ◽  
Prostaglandin D2 ◽  
Leukotriene C4 ◽  
Human Lung Tissue ◽  
No Release

1. In this study mast cells were found to comprise 2.1% of total cells recovered by enzymatic digestion of human lung tissue. 2. This mast cell population consisted of 79% formalin-sensitive, Alcian Blue-positive mast cells and 21% formalin-insensitive, Alcian Blue-positive mast cells. 3. By the use of centrifugal elutriation and subsequent Percoll gradient centrifugation, separate mixed cell populations could be obtained in which the mast cell constituents were either of the formalin-sensitive or -insensitive type. 4. Cell suspensions in which formalin-sensitive cells comprised 97% of mast cells contained approximately 1.34 pg of histamine per mast cell, whereas in preparations in which mast cells were 84% formalin-resistant the histamine content was approximately 4.17 pg of histamine per mast cell. 5. The histamine release upon anti-immunoglobulin E challenge of formalin-sensitive mast cells was greater than the release by formalin-insensitive mast cells. 6. After challenge with opsonized zymosan, only formalin-sensitive mast cells were able to release histamine. 7. Leukotriene C4 release was observed when formalin-sensitive mast cells were challenged with antiimmunoglobulin E. Formalin-insensitive mast cells showed no release of leukotriene C4. 8. Prostaglandin D2 release was observed when formalin-insensitive mast cells were challenged with antiimmunoglobulin E. Formalin-sensitive mast cells showed no release of prostaglandin D2.

scholarly journals Abnormal mast cell granules in the beige (Chédiak-Higashi syndrome) mouse.

1975 ◽  
Vol 23 (2) ◽  
pp. 117-122 ◽  
Author(s):  
E Y Chi ◽  
D Lagunoff
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Toluidine Blue ◽  
Ruthenium Red ◽  
Chediak Higashi Syndrome

Mast cells of beige (C57BL/6J) (bg-j/bg-j) mice were examined histochemically and ultrastructurally. Mast cell granules in the beige mice were markedly enlarged and irregular in shape. Granule contents stained uniformly with acidified toluidine blue, but with ruthenium red and Alcian Blue-safranin, two components were evident. The rims of the abnormal granules stained with ruthenium red and with Alcian Blue; the centers of the granules were clear with ruthenium red and stained with safranin. Mast cell granules thus represent another abnormal organelle in the Chédiak-Higashi syndrome.

scholarly journals The presence of mast cell precursors in rat peripheral blood

Blood ◽  
1981 ◽  
Vol 58 (3) ◽  
pp. 544-551 ◽  
Author(s):  
D Zucker-Franklin ◽  
G Grusky ◽  
N Hirayama ◽  
E Schnipper
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Peripheral Blood ◽  
Periodic Acid ◽  
Phenotypic Expression ◽  
Soft Agar ◽  
Sulfated Polysaccharides ◽  
Periodic Acid Schiff ◽  
Agar Culture

Abstract Soft agar culture of mononuclear cell fractions prepared from rat peripheral blood yielded numerous colonies consisting of mast cells. The mast cell nature of the cells was established by ultrastructural and histochemical analyses as well as by the demonstration the the colonies contained histamine and that the cells possessed receptors for the Fc component of IgE. Stringent criteria for the distinction of mast cells from monocytes/macrophages that could have metachromatic inclusions were applied. The alcian-blue-safranin technique delineated the maturation of mast cell granules by showing the loss of alcian-blue and increase in safranin-positive organelles presumed to reflect the increase in N-sulfated polysaccharides representing heparin. The mast cells exhibited low or absent reactions for peroxidase, alpha-naphthyl butyrate, periodic acid Schiff, and Sudan black reacting lipid, whereas macrophages stained in parallel were positive for these substances. Since it is known that extracellular conditions may cause variations in phenotypic expression, the observations have led to the hypothesis that mast cells and macrophages may have a common precursor.

An improved method for staining mouse mast cells

1989 ◽  
Vol 67 (1) ◽  
pp. 226-227 ◽  
Author(s):  
M. Novak ◽  
S. Nombrado
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Connective Tissue ◽  
Alcian Blue ◽  
Cell Types ◽  
Mucosal Mast Cell ◽  
Improved Method ◽  
Tissue Mast Cell ◽  
Pathological Conditions

An improved method for staining mouse mast cells with alcian blue is reported. The reaction differentiates between mucosal mast cell and connective tissue mast cell types, especially under pathological conditions.

scholarly journals The presence of mast cell precursors in rat peripheral blood

Blood ◽  
1981 ◽  
Vol 58 (3) ◽  
pp. 544-551
Author(s):  
D Zucker-Franklin ◽  
G Grusky ◽  
N Hirayama ◽  
E Schnipper
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Peripheral Blood ◽  
Periodic Acid ◽  
Phenotypic Expression ◽  
Soft Agar ◽  
Sulfated Polysaccharides ◽  
Periodic Acid Schiff ◽  
Agar Culture

Soft agar culture of mononuclear cell fractions prepared from rat peripheral blood yielded numerous colonies consisting of mast cells. The mast cell nature of the cells was established by ultrastructural and histochemical analyses as well as by the demonstration the the colonies contained histamine and that the cells possessed receptors for the Fc component of IgE. Stringent criteria for the distinction of mast cells from monocytes/macrophages that could have metachromatic inclusions were applied. The alcian-blue-safranin technique delineated the maturation of mast cell granules by showing the loss of alcian-blue and increase in safranin-positive organelles presumed to reflect the increase in N-sulfated polysaccharides representing heparin. The mast cells exhibited low or absent reactions for peroxidase, alpha-naphthyl butyrate, periodic acid Schiff, and Sudan black reacting lipid, whereas macrophages stained in parallel were positive for these substances. Since it is known that extracellular conditions may cause variations in phenotypic expression, the observations have led to the hypothesis that mast cells and macrophages may have a common precursor.

scholarly journals Ο πιθανός ρόλος των γλυκοζαμινογλυκανών στη διαφοροδιαγνωστική προσέγγιση του προστατικού αδενοκαρκινώματος

2004 ◽  
Author(s):  
Χριστίνα Μάγκου
Keyword(s):  
Mast Cell ◽  
Alcian Blue ◽  
Chondroitin Sulphate ◽  
Mast Cell Tryptase

Είναι γνωστή η περιαδενική συσσώρευση της θειϊκής χονδροϊτίνης (CS) στην . καλοήθη υπερπλασία (ΒΡΗ) και στο αδενοκαρκίνωμα του προστάτη (PCa) καθώς και ο ρόλος της ως κακό προγνωστικό δείκτη στο PCa. Τα μαστοκύτταρα (MC) είναι υπεύθυνα άμεσα ή έμμεσα -μέσω των ινοβλαστών- για την παραγωγή της CS, ενώ η CS επηρεάζει τη διαφοροποίηση και την αποκοκκίωση των MC. Σκοπός της μελέτης αυτής είναι η διερεύνηση των μεταβολών της CS και των MC στην ΒΡΗ και στο PCa.Η μελέτη περιλαμβάνει 67 προστατικά παρασκευάσματα (16,4% διορθικές βιοψίες, 31,3% διουρηθρικές προστατεκτομές, 22,4% διακυστικές προστατεκτομές, 29,9% ριζικές προστατεκτομές) από ασθενείς ηλικίας 50-87 ετών με ΒΡΗ (46,3%) και PCa (53,7%). Πολλαπλές συνεχόμενες τομές παραφίνης βάφτηκαν με τις χρώσεις Alcian Blue pH 2.5 και May Grünwald Giemsa, και έγινε ανοσοϊστοχημεία για τα μονοκλωνικά αντισώματα mouse anti-chondroitin sulphate (anti-CS), clone CS-56 (Sigma-Aldrich, Inc.) και mouse anti-mast cell tryptase (anti-tryptase), clone AA1 (Neomarkers, Fremont, USA). Με ψηφιακή ανάλυση εικόνας έγινε προσδιορισμός της μέσης έκτασης του στρώματος που καταλαμβάνει η CS (CS-MA/field, CS- ΜΑ%), της μέσης οπτικής πυκνότητας της CS (CS-MOD), του μέσου αριθμού των MC (MC-MN/field, MC-MN/Sd area), της μέσης διαμέτρου και του μέσου εμβαδόν επιφάνειας (MC-MA) του κυτταροπλάσματος των MC. Τα αποτελέσματα (meaniSE) επεξεργάστηκαν στατιστικά με μη παραμετρικές μεθόδους (SPSS 10.0 for Windows). Όλες οι τιμές ρ<0,05 θεωρήθηκαν στατιστικά σημαντικές.Ο μέσος αριθμός των MC στο στρώμα μεταξύ των αδενίων ήταν μικρότερος στην ΒΡΗ απ’ό,τι στο PCa (ρ<0,05). Το μέγεθος των MCs ήταν μικρότερο στην ΒΡΗ απ’ό,τι στο PCa, στην περιοχή μεταξύ των αδενίων (ρ<0,05) αλλά και γύρω από τα αδένια (ρ<0,05). Σε όλα τα περιστατικά, ο μέσος αριθμός των MCs ήταν μεγαλύτερος γύρω από τα αδένια απ’ό,τι μεταξύ των αδενίων (ρ<0,001). Στην ομάδα PCa, το μέγεθος των MC γύρω από τα νεοπλασματικά αδένια ήταν μεγαλύτερο απ’ό,τι στην μεταξύ των νεοπλασματικών αδενίων περιοχή (ρ<0,05). Τα PCa σταδίου ρΤ2 σε σχέση με τα ρΤ3, είχαν μικρότερη CS-MOD (ρ<0,05) και περισσότερα MC τόσο γύρω από τα αδένια (ρ<0,05) όσο και μεταξύ των αδενίων (ρ<0,05). Στην ομάδα ΒΡΗ, η CS-MOD εμφανίζει ασθενή αρνητική συσχέτιση με τον αριθμό των MC γύρω από τα αδένια. Στην ομάδα PCa, η έκταση του στρώματος που καταλαμβάνει η CS εμφανίζει ασθενή θετική συσχέτιση με το μέγεθος των MC γύρω από τα αδένια, ενώ η CS-MOD εμφανίζει ασθενή αρνητική συσχέτιση με το μέγεθος των MC μεταξύ των αδενίων. Στις νεοπλασματικές περιοχές, εντοπίστηκαν MC με κοκκία σημασμένα με anti-CS.CS και MC εμφανίζουν παράλληλες μεταβολές. Τα MC του προστατικού στρώματος γύρω από τα αδένια φαίνεται να ευθύνονται περισσότερο -σε σχέση με τα MC του στρώματος μεταξύ των αδενίων- για την παραγωγή CS στις παθήσεις του προστάτη, και επιπλέον το μέγεθος τους φαίνεται να αντιπροσωπεύει το βαθμό ενεργοποίησής τους.

scholarly journals Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.

1985 ◽  
Vol 162 (3) ◽  
pp. 1025-1043 ◽  
Author(s):  
T Nakano ◽  
T Sonoda ◽  
C Hayashi ◽  
A Yamatodani ◽  
Y Kanayama ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cell ◽  
Mast Cells ◽  
Connective Tissue ◽  
Alcian Blue ◽  
Peritoneal Cavity ◽  
Muscularis Propria ◽  
Glandular Stomach ◽  
Tissue Type ◽  
Peritoneal Mast Cells

Both connective tissue mast cells and mast cells grown in vitro are derived from multipotential hematopoietic stem cells, but these two mast cell populations exhibit many differences in morphology, biochemistry, and function. We investigated whether the phenotype of cultured mast cells or their progeny was altered when the cells were transferred into different locations in vivo. Cultured mast cells were immature by ultrastructure, and stained with alcian blue but with neither safranin or berberine sulfate, a fluorescent dye that binds to the heparin of connective tissue mast cell granules. By contrast, mast cells recovered from the peritoneal cavity of congenitally mast cell-deficient (WB X C57BL/6)F1-W/Wv (WBB6F1-W/Wv) mice 10 wk after intraperitoneal injection of cultured WBB6F1-+/+ or C57BL/6-bgJ/bgJ mast cells stained with both safranin and berberine sulfate. Staining with berberine sulfate was prevented by treatment of the cells with heparinase but not chondroitinase ABC, suggesting that the adoptively transferred mast cell population had acquired the ability to synthesize and store heparin. Furthermore, the recovered mast cells were indistinguishable by ultrastructure from the normal mature peritoneal mast cells of WBB6F1-+/+ mice, and contained substantially more histamine than mast cells studied directly from culture. Intravenous injection of cultured mast cells resulted in the development of safranin-and berberine sulfate-positive mast cells in the peritoneal cavity, spleen, skin, and glandular stomach muscularis propria. Mast cells also developed on the glandular stomach mucosa, but these cells stained with alcian blue rather than safranin, and did not stain with berberine sulfate. This result suggests that cultured mast cells can give rise to mast cells of either the connective tissue type or mucosal phenotype, depending on anatomical location. Furthermore, transplantation of cultured mast cells into WBB6F1-W/Wv mice had no measurable effect on the anemia of the recipient mice, suggesting a possible strategy for repairing the mast cell deficiency of WBB6F1-W/Wv mice without affecting other bone marrow-derived populations such as erythrocytes. Intravenous injection of representative connective tissue type mast cells (30-50% pure peritoneal mast cells derived from WBB6F1-+/+ mice) gave results similar to those obtained with cultured mast cells: mast cells developing in the peritoneal cavity, skin, spleen, and glandular stomach muscularis propria of WBB6F1-W/Wv recipients stained with safranin and berberine sulfate, whereas mast cells developing in the mucosa of the glandular stomach stained only with alcian blue.(ABSTRACT TRUNCATED AT 400 WORDS)

Sign in / Sign up

Export Citation Format

Share Document