scholarly journals Control of SRF binding to CArG box chromatin regulates smooth muscle gene expression in vivo

2005 ◽  
Vol 116 (1) ◽  
pp. 36-48 ◽  
Author(s):  
O. G. McDonald
Keyword(s):  
Gene Expression ◽  
Smooth Muscle ◽  
Muscle Gene Expression ◽  
Carg Box ◽  
Muscle Gene

scholarly journals Kruppel-like Factor 4 Abrogates Myocardin-induced Activation of Smooth Muscle Gene Expression

2005 ◽  
Vol 280 (10) ◽  
pp. 9719-9727 ◽  
Author(s):  
Yan Liu ◽  
Sanjay Sinha ◽  
Oliver G. McDonald ◽  
Yueting Shang ◽  
Mark H. Hoofnagle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Smooth Muscle ◽  
Muscle Gene Expression ◽  
Muscle Gene

scholarly journals In vivo lineage conversion of vertebrate muscle into early endoderm-like cells

2019 ◽  
Author(s):  
Clyde Campbell ◽  
Joseph J. Lancman ◽  
Raquel Espin Palazon ◽  
Jonatan Matalonga ◽  
Jiaye He ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Activation ◽  
Cell Lineage ◽  
Muscle Gene Expression ◽  
Functional Studies ◽  
Differentiated Cells ◽  
Pluripotency Gene ◽  
Fundamental Insight ◽  
Muscle Gene

The extent to which differentiated cells, while remaining in their native microenvironment, can be reprogrammed to assume a different identity will reveal fundamental insight into cellular plasticity and impact regenerative medicine. To investigate in vivo cell lineage potential, we leveraged the zebrafish as a practical vertebrate platform to determine factors and mechanisms necessary to induce differentiated cells of one germ layer to adopt the lineage of another. We discovered that ectopic co-expression of Sox32 and Oct4 in several non-endoderm lineages, including skeletal muscle, can specifically trigger an early endoderm genetic program in a cell-autonomous manner. Gene expression, live imaging, and functional studies reveal that the endoderm-induced muscle cells lose muscle gene expression and morphology, while specifically gaining endoderm organogenesis markers, such as the pancreatic specification genes, hhex and ptf1a, via a mechanism resembling normal development. Endoderm induction by a pluripotent defective form of Oct4, endoderm markers appearing prior to loss of muscle cell morphology, a lack of dependence on cell division, and a lack of mesoderm, ectoderm, dedifferentiation, and pluripotency gene activation, together, suggests that reprogramming is endoderm specific and occurs via direct lineage conversion. Our work demonstrates that within a vertebrate animal, stably differentiated cells can be induced to directly adopt the identity of a completely unrelated cell lineage, while remaining in a distinct microenvironment, suggesting that differentiated cells in vivo may be more amenable to lineage conversion than previously appreciated. This discovery of possibly unlimited lineage potential of differentiated cells in vivo challenges our understanding of cell lineage restriction and may pave the way towards a vast new in vivo supply of replacement cells for degenerative diseases such as diabetes.

scholarly journals von Willebrand Factor Permeates Small Vessels in CADASIL and Inhibits Smooth Muscle Gene Expression

2011 ◽  
Vol 3 (1) ◽  
pp. 138-145 ◽  
Author(s):  
Xiaojie Zhang ◽  
He Meng ◽  
Mila Blaivas ◽  
Elisabeth J. Rushing ◽  
Brian E. Moore ◽  
...  
Keyword(s):  
Gene Expression ◽  
Smooth Muscle ◽  
Von Willebrand Factor ◽  
Small Vessels ◽  
Muscle Gene Expression ◽  
Muscle Gene ◽  
Von Willebrand ◽  
Willebrand Factor
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