scholarly journals Disruption of cytoskeletal structures mediates shear stress-induced endothelin-1 gene expression in cultured porcine aortic endothelial cells.

1993 ◽  
Vol 92 (4) ◽  
pp. 1706-1712 ◽  
Author(s):  
T Morita ◽  
H Kurihara ◽  
K Maemura ◽  
M Yoshizumi ◽  
Y Yazaki
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Aortic Endothelial Cells ◽  
Endothelin 1 ◽  
Porcine Aortic Endothelial Cells ◽  
Aortic Endothelial

scholarly journals Flow-dependent Smad2 phosphorylation and TGIF nuclear localization in human aortic endothelial cells

2011 ◽  
Vol 301 (1) ◽  
pp. H98-H107 ◽  
Author(s):  
Robert D. Shepherd ◽  
Stephanie M. Kos ◽  
Kristina D. Rinker
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Fluid Flow ◽  
Nuclear Localization ◽  
Aortic Endothelial Cells ◽  
Linker Region ◽  
Stress Dependent ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial

Endothelial cells respond to fluid flow stimulation through transient and sustained signal pathway activation. Smad2 is a signaling molecule and transcription factor in the Smad signaling pathway, traditionally associated with TGF-β. Although phosphorylation of Smad2 in the receptor-dependent COOH-terminal region is the most appreciated way Smad2 is activated to affect gene expression, phosphorylation may also occur in the MH1-MH2 linker region (L-psmad2). Here, we show that in human aortic endothelial cells (HAEC), Smad2 was both preferentially phosphorylated in the linker region and localized to the nucleus in a flow-dependent manner. The Smad corepressor transforming growth interacting factor (TGIF) was also found to have flow-dependent nuclear localization. Tissue studies confirmed this L-psmad2 generation trend in rat aorta, indicating likely importance in arterial tissue. HAEC-based inhibitor studies demonstrated that L-psmad2 levels were not related to MAPK phosphorylation, but instead followed the pattern of pAkt473, both with and without the phosphatidylinositol 3-kinase inhibitor PI-103. Akt and Smad species were also shown to directly interact under flow relative to static controls. To further evaluate impacts of PI-103 treatment, expression profiles for two TGF-β and shear stress-dependent genes were determined and showed that mRNAs were lower from untreated 10 dyn/cm2than 2 dyn/cm2average shear stress cultures. However, upon exposure to PI-103, this trend was reversed, with a stronger response observed at 10 dyn/cm2. Taken together, the results of this work suggest that fluid flow exposure may influence endothelial gene expression by a novel mechanism involving Akt, L-psmad2, and TGIF.

Conversion of big endothelin-1 to endothelin-1 by two types of metalloproteinases derived from porcine aortic endothelial cells

FEBS Letters ◽  
1990 ◽  
Vol 272 (1-2) ◽  
pp. 166-170 ◽  
Author(s):  
Yasuo Matsumura ◽  
Ruriko Ikegawa ◽  
Yaeko Tsukahara ◽  
Masanori Takaoka ◽  
Shiro Morimoto
Keyword(s):  
Endothelial Cells ◽  
Aortic Endothelial Cells ◽  
Endothelin 1 ◽  
Porcine Aortic Endothelial Cells ◽  
Aortic Endothelial

Effect of glucose and insulin on immunoreactive endothelin-1 release from cultured porcine aortic endothelial cells

Metabolism ◽  
1991 ◽  
Vol 40 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Yoshiyuki Hattori ◽  
Kikuo Kasai ◽  
Tsutomu Nakamura ◽  
Tatsushi Emoto ◽  
Shin-Ichi Shimoda
Keyword(s):  
Endothelial Cells ◽  
Aortic Endothelial Cells ◽  
Endothelin 1 ◽  
Effect Of Glucose ◽  
Porcine Aortic Endothelial Cells ◽  
Aortic Endothelial
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