scholarly journals Gene silencing of endothelial von Willebrand Factor attenuates angiotensin II-induced endothelin-1 expression in porcine aortic endothelial cells

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Anar Dushpanova ◽  
Silvia Agostini ◽  
Enrica Ciofini ◽  
Manuela Cabiati ◽  
Valentina Casieri ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Angiotensin Ii ◽  
Gene Silencing ◽  
Von Willebrand Factor ◽  
Aortic Endothelial Cells ◽  
Endothelin 1 ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Porcine Aortic Endothelial Cells ◽  
Aortic Endothelial

scholarly journals The Interaction of the Platelet and von Willebrand Factor

1977 ◽  
Author(s):  
D.N. Fass ◽  
F. Booyse ◽  
J.C. Lewis ◽  
E. J. W. Bowie
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Cultures ◽  
Von Willebrand Factor ◽  
Sandwich Technique ◽  
Aortic Endothelial Cells ◽  
Confluent Culture ◽  
Endothelial Cell Cultures ◽  
Von Willebrand ◽  
Willebrand Factor

A culture of pig aortic endothelial cells was used for experiments to investigate the interaction between the platelet and von Willebrand factor. An antibody was raised in rabbits to purified porcine von Willebrand factor. A semi-confluent culture of pig endothelial cells was stained immunofluorescently by the sandwich technique using anti-Willebrand factor IgG. An extensive extracellular meshwork of microfilaments was revealed. In endothelial cell cultures from von Willebrand pigs, no immunoreactive microfilaments were found. Immunoelectronmicro-scopy with peroxidase linked antibody has been used to identify similar filaments in normal pig endothelial cells. Washed platelets were shown to adhere to semiconfluent or damaged normal endothelial cell cultures. If the cultures had been previously incubated with anti-Willebrand factor IgG, the washed platelets did not adhere. There was no adherence of platelets when they were added to semiconfluent or damaged von Willebrand endothelial cells.

Conversion of big endothelin-1 to endothelin-1 by two types of metalloproteinases derived from porcine aortic endothelial cells

FEBS Letters ◽  
1990 ◽  
Vol 272 (1-2) ◽  
pp. 166-170 ◽  
Author(s):  
Yasuo Matsumura ◽  
Ruriko Ikegawa ◽  
Yaeko Tsukahara ◽  
Masanori Takaoka ◽  
Shiro Morimoto
Keyword(s):  
Endothelial Cells ◽  
Aortic Endothelial Cells ◽  
Endothelin 1 ◽  
Porcine Aortic Endothelial Cells ◽  
Aortic Endothelial

Effect of glucose and insulin on immunoreactive endothelin-1 release from cultured porcine aortic endothelial cells

Metabolism ◽  
1991 ◽  
Vol 40 (2) ◽  
pp. 165-169 ◽  
Author(s):  
Yoshiyuki Hattori ◽  
Kikuo Kasai ◽  
Tsutomu Nakamura ◽  
Tatsushi Emoto ◽  
Shin-Ichi Shimoda
Keyword(s):  
Endothelial Cells ◽  
Aortic Endothelial Cells ◽  
Endothelin 1 ◽  
Effect Of Glucose ◽  
Porcine Aortic Endothelial Cells ◽  
Aortic Endothelial

scholarly journals Cultured Normal and von Willebrand Porcine Aortic Endothelial Cells-An in Vitro Model for Studying the Nature and Site of Platelet-Endothelial Component Interaction

1977 ◽  
Author(s):  
F.M. Booyse ◽  
D.N. Fass ◽  
E.J.W. Bowie
Keyword(s):  
Endothelial Cells ◽  
In Vitro Model ◽  
Divalent Cations ◽  
Aortic Endothelial Cells ◽  
Vitro Model ◽  
Wall Interaction ◽  
Von Willebrand ◽  
Porcine Aortic Endothelial Cells ◽  
Aortic Endothelial

Normal and von Willebrand(vWd) porcine aortic endothelial cells(EC) have been maintained and subcultured for10-12 months(14-18 passages) without any apparent change in characteristic growth morphology or ultrastructure. Immunofluorescence staining ofthese EC for ristocetin-Willebrand factor(RWF), using monospecific rabbit antiporcine RWF(aRWF), showed differences in the extent and nature of intercellular staining and the apparent absence of extracellular EC associated RWF-containing material(filaments)in vWd cells. Platelet-endothelial(damaged) interaction was decreased in normal EC cultures by pretreatment of the cultures with aRWF, no platelet interaction was seen with untreated vWd EC. These cultures were used as an in vitro model system(optimal pH, divalent cations, protein, exposure time and rotation speed)for studying the nature, extent and differencesin the platelet-microfi1ament interaction in normal and vWd EC. Preliminary data on the microfilament site of attachment of washed platelets or antibody-coupled beads, the interaction of platelets with matrix-bound purified RWF and the presence of immunologically identifiable RWF-containing filaments in normal and vWd EC suggest a possible role for endothelial-bound RWF in platelet-vessel wall interaction.

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