scholarly journals Effect of secretion on intracellular pH regulation in isolated rat bile duct epithelial cells.

1993 ◽  
Vol 92 (3) ◽  
pp. 1314-1325 ◽  
Author(s):  
D Alvaro ◽  
W K Cho ◽  
A Mennone ◽  
J L Boyer
Keyword(s):  
Bile Duct ◽  
Epithelial Cells ◽  
Intracellular Ph ◽  
Ph Regulation ◽  
Intracellular Ph Regulation ◽  
Rat Bile ◽  
Isolated Rat

scholarly journals Intracellular pH regulation in bombesin-stimulated secretion in isolated bile duct units from rat liver

1998 ◽  
Vol 275 (5) ◽  
pp. G1028-G1036 ◽  
Author(s):  
Won Kyoo Cho ◽  
Albert Mennone ◽  
James L. Boyer
Keyword(s):  
Bile Duct ◽  
Ion Transport ◽  
Intracellular Ph ◽  
Rat Liver ◽  
Ph Regulation ◽  
Transport Processes ◽  
Intracellular Ph Regulation ◽  
Normal Rat ◽  
Underlying Mechanisms ◽  
Exchange Activity

Bombesin, a neuropeptide, stimulates fluid and [Formula: see text] secretion from cholangiocytes, but the underlying mechanisms are poorly understood. In this study, we aimed to examine the effects of bombesin on ion transport processes involved in the regulation of intracellular pH (pHi) and[Formula: see text] secretion in polarized cholangiocytes. Isolated bile duct units from normal rat liver were used to measure pHiby 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein 495 nm-to-440 nm dual ratio methods. Bombesin increased Cl−-[Formula: see text] exchange activity but did not affect basal pHior the activities of Na+/H+exchange or Na+-[Formula: see text] symport. Depolarization of cholangiocytes increased basal pHiand the activity of Cl−/[Formula: see text]exchange, suggesting that an electrogenic Na+-[Formula: see text]symport might function as a counterregulatory pHimechanism. Na+-independent acid-extruding mechanisms were not observed. We conclude that bombesin stimulates biliary secretion from cholangiocytes by activating luminal Cl−/[Formula: see text]exchange, which may be coupled to basolateral electrogenic Na+-[Formula: see text] symport.

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