Intracellular pH regulation and buffer capacity in CO 2 /HCO − 3 -buffered media in cultured epithelial cells from rainbow trout gills

2000 ◽  
Vol 170 (3) ◽  
pp. 175-184 ◽  
Author(s):  
C. M. Wood ◽  
P. Pärt
Keyword(s):  
Rainbow Trout ◽  
Epithelial Cells ◽  
Intracellular Ph ◽  
Buffer Capacity ◽  
Ph Regulation ◽  
Intracellular Ph Regulation ◽  
Cultured Epithelial Cells

scholarly journals Myocardial intracellular pH in a perfused rainbow trout heart during extracellular acidosis in the presence and absence of adrenaline

1986 ◽  
Vol 125 (1) ◽  
pp. 347-359 ◽  
Author(s):  
A. P. Farrell ◽  
C. L. Milligan
Keyword(s):  
Rainbow Trout ◽  
Intracellular Ph ◽  
Ph Regulation ◽  
Respiratory Acidosis ◽  
Mechanical Efficiency ◽  
Extracellular Ph ◽  
Salmo Gairdneri ◽  
Intracellular Ph Regulation ◽  
Extracellular Acidosis

Myocardial intracellular pH was measured in a perfused rainbow trout, Salmo gairdneri, with DMO (5,5-dimethyl-2,4-oxazlidinedione), to test the hypothesis that catecholamines promote active regulation of myocardial pH in order to protect contractility during a respiratory acidosis comparable to that observed after exercise. Under control conditions (extracellular pH = 8.0; PCO2 = 2 Torr), myocardial pH was 7.53 +/− 0.01 (N = 5). Acidosis (extracellular pH = 7.45; PCO2 = 8.6 Torr) reduced contractility, mechanical efficiency and intracellular pH (7.25 +/− 0.04), but did not affect myocardial O2 consumption. The addition of 0.5 mumol l-1 adrenaline during extracellular acidosis prevented the loss of contractility, restored mechanical efficiency, but did not change intracellular pH significantly. Thus, adrenaline enabled cardiac contractility to recover, without intracellular pH regulation, possibly by modulation of sarcolemmal calcium changes. The absence of a myocardial acidosis after exercise in vivo is discussed with respect to possible intracellular pH regulation via lactate uptake and metabolism.

Effect of hypercapnia on intracellular pH regulation in a rainbow trout hepatoma cell line, RTH 149

2011 ◽  
Vol 181 (7) ◽  
pp. 883-892 ◽  
Author(s):  
Khuong Tuyen Huynh ◽  
Daniel W. Baker ◽  
Robert Harris ◽  
John Church ◽  
Colin J. Brauner
Keyword(s):  
Rainbow Trout ◽  
Cell Line ◽  
Intracellular Ph ◽  
Ph Regulation ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Intracellular Ph Regulation
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