scholarly journals Potent mitogenic effects of parathyroid hormone (PTH) on embryonic chick and rabbit chondrocytes. Differential effects of age on growth, proteoglycan, and cyclic AMP responses of chondrocytes to PTH.

1990 ◽  
Vol 85 (3) ◽  
pp. 626-631 ◽  
Author(s):  
T Koike ◽  
M Iwamoto ◽  
A Shimazu ◽  
K Nakashima ◽  
F Suzuki ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Embryonic Chick ◽  
Differential Effects

Development of the cyclic AMP response to parathyroid hormone and prostaglandin E2 in the embryonic chick limb

1981 ◽  
Vol 33 (1) ◽  
pp. 641-648 ◽  
Author(s):  
Curtis L. Parker ◽  
David M. Biddulph ◽  
Timothy A. Ballard
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Prostaglandin E2 ◽  
Embryonic Chick

Interaction between parathyroid hormone and prostaglandin E1 on cyclic AMP metabolism in rat kidney

1980 ◽  
Vol 93 (3) ◽  
pp. 339-345 ◽  
Author(s):  
Naokazu Nagata ◽  
Yuriko Ono ◽  
Narimichi Kimura
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Rat Kidney ◽  
Prostaglandin E ◽  
Cortical Tissue ◽  
Newborn Rat ◽  
Simultaneous Addition ◽  
Renal Cortical Tissue ◽  
Subsequent Addition

Abstract. The interaction between parathyroid hormone (PTH) and prostaglandin E1 (PGE1) in influencing cyclic AMP metabolism in rat renal cortical tissue was examined. PTH and PGE1 stimulated additively the adenylate cyclase activity in the homogenate of the tissue. Both PTH and PGE1 enhanced the level of cyclic AMP in the incubated renal cortical tissue, but the effect of their simultaneous addition did not exceed the effect induced by PTH alone. Cyclic AMP accumulated in the incubation medium by stimulation by PTH was decreased by the simultaneous addition of PGE1. When the tissue was pre-incubated for 30 min with 2 to 10 μg/ml of PGE1, the magnitude of the increase of cyclic AMP caused by PTH subsequently added was lessened. However, the response to PTH of adenylate cyclase preparation obtained from the homogenate of PGE1-pre-treated tissue was not decreased. When first PTH was added to the incubating renal cortical tissue, the subsequent addition of PGE1 accelerated the decrease of cyclic AMP content in the tissue and decreased the amount of cyclic AMP released from the tissue. The interaction of PTH and PGE1 on cyclic AMP metabolism in the renal cortical tissue was in contrast to that seen in newborn rat calvaria where PGE1 and PTH acted additively in enhancing the level of cyclic AMP.

Species differences in the effect of parathyroid hormone on cyclic amp production

1980 ◽  
Vol 67 (3) ◽  
pp. 471-475 ◽  
Author(s):  
I. Lewin ◽  
S. Tomlinson ◽  
G.N. Hendy ◽  
J.L.H. O'Riordan ◽  
D.W. Pickard ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Species Differences

scholarly journals Pseudohypoparathyroidism Showing Positive Phosphaturic and Negative Cyclic AMP Excretion Response to Parathyroid Hormone.

1989 ◽  
Vol 36 (4) ◽  
pp. 465-469 ◽  
Author(s):  
KIICHIRO HIGASHI ◽  
KENICHI HONDA ◽  
MITSUO MORITA ◽  
TERUHISA UMEDA ◽  
TATSUYA SHIMADA ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp

scholarly journals Parathyroid hormone-related protein detection and interaction with NO and cyclic AMP in the renovascular system

1996 ◽  
Vol 50 (5) ◽  
pp. 1591-1603 ◽  
Author(s):  
Thierry Massfelder ◽  
Andrew F. Stewart ◽  
Karlhans Endlich ◽  
Neil Soifer ◽  
Clément Judes ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Protein Detection ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein

Studies on the use of cultured cells in a bioassay for parathyroid hormone

1994 ◽  
Vol 143 (2) ◽  
pp. 261-268
Author(s):  
A E Armston ◽  
P J Wood
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Cell Line ◽  
Cultured Cells ◽  
Biologically Active ◽  
Kidney Cell Line ◽  
Opossum Kidney ◽  
Pre Treatment ◽  
Set Up ◽  
Insufficient Sensitivity

Abstract Measurement of parathyroid hormone (PTH) is important for diagnosing hyper- and hypoparathyroidism. The move to two-site immunometric assays that detect the whole molecule has improved the discrimination of these conditions but these assays may be too restrictive because some PTH fragments that are biologically active may not be detected. In addition, PTH-like peptide of malignancy, an important cause of malignancy-associated hypercalcaemia, is not detected by the two-site assays. Experiments were performed to set up a simple, robust and inexpensive bioassay for PTH, exploiting a kidney cell line and using cyclic AMP or an eluted stain assay as the end point. Of the 12 cell lines tested, an opossum kidney (WOK) cell line showed the most promise. Despite optimization of the procedure to include pre-treatment with dexamethasone, insulin and PTH, followed by incubation in the presence of 5′ -guanylimidodiphosphate, isobutyl-1-methylxanthine and forskolin, the WOK cells showed insufficient sensitivity for use in a cultured cell bioassay for PTH in human serum. In addition, the cells were less sensitive to PTH-like peptide precluding their use for an assay for this molecule. Journal of Endocrinology (1994) 143, 261–268

Cyclic Amp Response to Parathyroid Hormone during Lithium Treatment

1982 ◽  
Vol 62 (2) ◽  
pp. 34P-34P
Author(s):  
D.G. Waller ◽  
J.D.M. Albano ◽  
J.G.B. Millar ◽  
A. Polak
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Lithium Treatment
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