scholarly journals Leukotriene C4 binds to human glomerular epithelial cells and promotes their proliferation in vitro.

1985 ◽  
Vol 76 (1) ◽  
pp. 374-377 ◽  
Author(s):  
L Baud ◽  
J Sraer ◽  
J Perez ◽  
M P Nivez ◽  
R Ardaillou
Keyword(s):  
Epithelial Cells ◽  
Leukotriene C4 ◽  
Glomerular Epithelial Cells ◽  
Proliferation In Vitro

scholarly journals In vitro incubation and study of kidney glomerular epithelial cells

1979 ◽  
Vol 15 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Peter M. Andrews ◽  
Marguerite Stauver
Keyword(s):  
Epithelial Cells ◽  
In Vitro Incubation ◽  
Glomerular Epithelial Cells

Construction of glomerular epithelial cells in vitro for removal of immune complex

1999 ◽  
Vol 2 (2) ◽  
pp. 170-175 ◽  
Author(s):  
Pi-Chao Wang ◽  
Kenichi Horisawa ◽  
Masatoshi Matsumura
Keyword(s):  
Epithelial Cells ◽  
Immune Complex ◽  
Glomerular Epithelial Cells

scholarly journals Urokinase-plasminogen activator is synthesized in vitro by human glomerular epithelial cells but not by mesangial cells

1994 ◽  
Vol 45 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Paul A.J. Brown ◽  
Heather M. Wilson ◽  
Fiona J. Reid ◽  
Nuala A. Booth ◽  
John G. Simpson ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Plasminogen Activator ◽  
Mesangial Cells ◽  
Urokinase Plasminogen Activator ◽  
Glomerular Epithelial Cells

p38 Mitogen-activated protein kinase protects glomerular epithelial cells from complement-mediated cell injury

2003 ◽  
Vol 285 (4) ◽  
pp. F765-F774 ◽  
Author(s):  
Lamine Aoudjit ◽  
Monica Stanciu ◽  
Hongping Li ◽  
Serge Lemay ◽  
Tomoko Takano
Keyword(s):  
Protein Kinase ◽  
Epithelial Cells ◽  
P38 Mapk ◽  
Cell Injury ◽  
Transforming Growth Factor ◽  
Cultured Cells ◽  
Mitogen Activated Protein Kinase ◽  
Control Treatment ◽  
Glomerular Epithelial Cells

In the passive Heymann nephritis (PHN) model of rat membranous nephropathy, complement C5b-9 causes sublytic injury of glomerular epithelial cells (GEC). We previously showed that sublytic concentration of C5b-9 triggers a variety of biological events in GEC. In the current study, we demonstrate that complement activates p38 MAPK in GEC and address the role of p38 in complement-mediated cell injury. When cultured rat GEC were stimulated with complement, p38 kinase activity and phosphorylation were increased by ∼2.4-fold, compared with control. Treatment with p38 inhibitors significantly augmented complement-mediated cytotoxicity. In contrast, when the constitutively active mutant of transforming growth factor-β-activated kinase 1 (TAK1), a kinase upstream of p38, was expressed in GEC in an inducible manner, cytotoxicity was significantly reduced, compared with uninduced cells. p38 inhibitors abolished the protective effect of TAK1 expression. By analogy to cultured cells, p38 activity was also increased in glomeruli from rats with PHN and treatment with the p38 inhibitor FR-167653 increased proteinuria. Complement induced phosphorylation of MAPK-associated protein kinase-2 (MAPKAPK-2), a kinase downstream of p38 in GEC. Heat shock protein (HSP27) is a cytoskeleton-interacting substrate of MAPKAPK-2. Overexpression of the wild-type HSP27, but not a non-phosphorylatable mutant, markedly reduced complement-mediated GEC injury. In summary, complement activates p38 MAPK in GEC in vitro and in glomeruli from rats with PHN. The activation of p38 MAPK appears to be cytoprotective for GEC against complement-mediated GEC injury. Phosphorylation of HSP27 may mediate this cytoprotection.

Expression of neuropilin-1 by human glomerular epithelial cells in vitro and in vivo

2001 ◽  
Vol 101 (4) ◽  
pp. 439-446 ◽  
Author(s):  
Steven J. HARPER ◽  
Chang Ying XING ◽  
Cathy WHITTLE ◽  
Robin PARRY ◽  
David GILLATT ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Reverse Transcription ◽  
Target Cells ◽  
Normal Kidney ◽  
Autocrine Loop ◽  
Reverse Transcription Pcr ◽  
Neuropilin 1 ◽  
Glomerular Epithelial Cells

Vascular endothelial growth factor (VEGF) is a potent promoter of endothelial mitogenesis and of endothelial permeability. Within the kidney it is synthesized primarily in the visceral glomerular epithelial cells (vGECs); however, the role of VEGF in the glomerulus remains unknown, as does the target cell upon which it acts. Although the target cells may be those of the glomerular endothelium, there are micro-anatomical reasons why this might not be the case. This, therefore, led us to consider the possibility that glomerular VEGF may bind to the vGECs themselves. Since it has been shown that vGECs do not express the main tyrosine kinase VEGF receptors, we chose to study vGEC expression of the more recently described VEGF isoform-specific receptors, the neuropilins. The expression of mRNAs for neuropilin-1, neuropilin-2 and soluble neuropilin was studied in whole kidney, sieved glomeruli and cultured podocytes by reverse transcription-PCR, and neuropilin-1 mRNA expression in isolated single glomeruli was analysed by nested reverse transcription-PCR. The expression of neuropilin-1 protein was investigated in cultured vGECs by Western blotting and immunocytochemistry, and in normal kidney sections by immunohistochemistry. Neuropilin-1 mRNA was detected in whole kidney, single and sieved glomeruli and cultured vGECs. Neuropilin-1 protein was detected in cultured vGECs and in vGECs in normal kidney sections by immunohistochemistry. Thus the present study suggests that vGECs may have the potential to bind the VEGF that they secrete. Functional studies will be required to address the potential significance of this finding in terms of an autocrine loop or VEGF sequestration.

Retinoic Acid Enhances the Number of Epidermal Growth Factor Receptors in Rat Glomerular Epithelial Cells in vitro

Nephron ◽  
1994 ◽  
Vol 68 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Makoto Kawaguchi ◽  
Fumie Kawashima ◽  
Keisuke Ohshima ◽  
Satoru Kawaguchi ◽  
Hiroyoshi Wada
Keyword(s):  
Retinoic Acid ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
Growth Factor Receptors ◽  
Epidermal Growth Factor Receptors ◽  
Glomerular Epithelial Cells ◽  
Epidermal Growth

Ferric cacodylate efficiently stimulates growth of rat renal glomerular epithelial cells in vitro

1990 ◽  
Vol 3 (3) ◽  
pp. 245-251
Author(s):  
Masayasu Yamada ◽  
Chiharu Moritoh ◽  
Tohru Okigaki
Keyword(s):  
Epithelial Cells ◽  
Glomerular Epithelial Cells

Expression of neuropilin-1 by human glomerular epithelial cells in vitro and in vivo

2001 ◽  
Vol 101 (4) ◽  
pp. 439 ◽  
Author(s):  
Steven J. HARPER ◽  
Chang Ying XING ◽  
Cathy WHITTLE ◽  
Robin PARRY ◽  
David GILLATT ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Neuropilin 1 ◽  
Glomerular Epithelial Cells

Multiple mechanisms for doxorubicin cytotoxicity on glomerular epithelial cells ‘in vitro’

1992 ◽  
Vol 228 (2-3) ◽  
pp. 77-83 ◽  
Author(s):  
Gian Marco Ghiggeri ◽  
Roberta Bertelli ◽  
Fabrizio Ginevri ◽  
Roberta Oleggini ◽  
Paola Altieri ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Glomerular Epithelial Cells
Sign in / Sign up

Export Citation Format

Share Document