Expression of neuropilin-1 by human glomerular epithelial cells in vitro and in vivo

2001 ◽  
Vol 101 (4) ◽  
pp. 439 ◽  
Author(s):  
Steven J. HARPER ◽  
Chang Ying XING ◽  
Cathy WHITTLE ◽  
Robin PARRY ◽  
David GILLATT ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Neuropilin 1 ◽  
Glomerular Epithelial Cells

Expression of neuropilin-1 by human glomerular epithelial cells in vitro and in vivo

2001 ◽  
Vol 101 (4) ◽  
pp. 439-446 ◽  
Author(s):  
Steven J. HARPER ◽  
Chang Ying XING ◽  
Cathy WHITTLE ◽  
Robin PARRY ◽  
David GILLATT ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Reverse Transcription ◽  
Target Cells ◽  
Normal Kidney ◽  
Autocrine Loop ◽  
Reverse Transcription Pcr ◽  
Neuropilin 1 ◽  
Glomerular Epithelial Cells

Vascular endothelial growth factor (VEGF) is a potent promoter of endothelial mitogenesis and of endothelial permeability. Within the kidney it is synthesized primarily in the visceral glomerular epithelial cells (vGECs); however, the role of VEGF in the glomerulus remains unknown, as does the target cell upon which it acts. Although the target cells may be those of the glomerular endothelium, there are micro-anatomical reasons why this might not be the case. This, therefore, led us to consider the possibility that glomerular VEGF may bind to the vGECs themselves. Since it has been shown that vGECs do not express the main tyrosine kinase VEGF receptors, we chose to study vGEC expression of the more recently described VEGF isoform-specific receptors, the neuropilins. The expression of mRNAs for neuropilin-1, neuropilin-2 and soluble neuropilin was studied in whole kidney, sieved glomeruli and cultured podocytes by reverse transcription-PCR, and neuropilin-1 mRNA expression in isolated single glomeruli was analysed by nested reverse transcription-PCR. The expression of neuropilin-1 protein was investigated in cultured vGECs by Western blotting and immunocytochemistry, and in normal kidney sections by immunohistochemistry. Neuropilin-1 mRNA was detected in whole kidney, single and sieved glomeruli and cultured vGECs. Neuropilin-1 protein was detected in cultured vGECs and in vGECs in normal kidney sections by immunohistochemistry. Thus the present study suggests that vGECs may have the potential to bind the VEGF that they secrete. Functional studies will be required to address the potential significance of this finding in terms of an autocrine loop or VEGF sequestration.

Three-dimensional Growth of Renal Epithelial Cells in Vitro: A Tool in Toxicity Testing

1993 ◽  
Vol 21 (2) ◽  
pp. 191-195 ◽  
Author(s):  
Knut-Jan Andersen ◽  
Erik Ilsø Christensen ◽  
Hogne Vik
Keyword(s):  
Epithelial Cells ◽  
Three Dimensional ◽  
Toxicity Testing ◽  
Renal Tissue ◽  
Epithelial Cell Line ◽  
Multicellular Spheroids ◽  
Renal Epithelial Cell ◽  
Renal Epithelial Cells

The tissue culture of multicellular spheroids from the renal epithelial cell line LLC-PK1 (proximal tubule) is described. This represents a biological system of intermediate complexity between renal tissue in vivo and simple monolayer cultures. The multicellular structures, which show many similarities to kidney tubules in vivo, including a vectorial water transport, should prove useful for studying the potential nephrotoxicity of drugs and chemicals in vitro. In addition, the propagation of renal epithelial cells as multicellular spheroids in serum-free culture may provide information on the release of specific biological parameters, which may be suppressed or masked in serum-supplemented media.

Neuropilin-1 on hematopoietic cells as a source of vascular development

Blood ◽  
2003 ◽  
Vol 101 (5) ◽  
pp. 1801-1809 ◽  
Author(s):  
Yoshihiro Yamada ◽  
Yuichi Oike ◽  
Hisao Ogawa ◽  
Yasuhiro Ito ◽  
Hajime Fujisawa ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Fetal Liver ◽  
Vascular Development ◽  
Hematopoietic Cells ◽  
Vegf Receptor ◽  
Knock Out ◽  
Neuropilin 1 ◽  
Vasculogenesis And Angiogenesis

Neuropilin-1 (NP-1) is a receptor for vascular endothelial growth factor-165 (VEGF165) and acts as a coreceptor that enhances the function of VEGF165 through VEGF receptor-2 (VEGFR-2). Studies using transgenic and knock-out mice of NP-1 indicated that this molecule is important for vascular development as well as neuronal development. We recently reported that clustered soluble NP-1 phosphorylates VEGFR-2 on endothelial cells with a low dose of VEGF165 and rescues the defective vascularity of the NP-1−/− embryo in vitro and in vivo. Here we show that NP-1 is expressed by CD45+ hematopoietic cells in the fetal liver, can bind VEGF165, and phosphorylates VEGFR-2 on endothelial cells. CD45+NP-1+ cells rescued the defective vasculogenesis and angiogenesis in the NP-1−/− P-Sp (para-aortic splanchnopleural mesodermal region) culture, although CD45+NP-1− cells did not. Moreover, CD45+NP-1+ cells together with VEGF165 induced angiogenesis in an in vivo Matrigel assay and cornea neovascularization assay. The extracellular domain of NP-1 consists of “a,” “b,” and “c” domains, and it is known that the “a” and “c” domains are necessary for dimerization of NP-1. We found that both the “a” and “c” domains are essential for such rescue of defective vascularities in the NP-1 mutant. These results suggest that NP-1 enhances vasculogenesis and angiogenesis exogenously and that dimerization of NP-1 is important for enhancing vascular development. In NP-1−/− embryos, vascular sprouting is impaired at the central nervous system (CNS) and pericardium where VEGF is not abundant, indicating that NP-1–expressing cells are required for normal vascular development.

scholarly journals In vitro incubation and study of kidney glomerular epithelial cells

1979 ◽  
Vol 15 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Peter M. Andrews ◽  
Marguerite Stauver
Keyword(s):  
Epithelial Cells ◽  
In Vitro Incubation ◽  
Glomerular Epithelial Cells

ErbB-2 Induces the Cyclin D1 Gene in Prostate Epithelial Cells In vitro and In vivo

2007 ◽  
Vol 67 (9) ◽  
pp. 4364-4372 ◽  
Author(s):  
Mathew Casimiro ◽  
Olga Rodriguez ◽  
Llana Pootrakul ◽  
Maral Aventian ◽  
Nadia Lushina ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cyclin D1 ◽  
Prostate Epithelial Cells

In vitro and in vivo pathologic effects of Vibrio parahaemolyticus on human epithelial cells

1984 ◽  
Vol 30 (3) ◽  
pp. 381-388 ◽  
Author(s):  
B. R. Merrell ◽  
R. I. Walker ◽  
S. W. Joseph
Keyword(s):  
Epithelial Cells ◽  
Epithelial Tissue ◽  
Ileal Mucosa ◽  
Cytotoxic Factor ◽  
Culture Cells ◽  
Vibrio Parahemolyticus ◽  
Buccal Epithelial Cells ◽  
Culture Supernate

The initial interaction and adherence of Vibrio parahemolyticus to epithelial tissue culture cells, human buccal epithelial cells, and the ileal mucosa of mice were studied. Using scanning electron microscopy, adherent bacteria were observed only on degenerating human embryonic intestinal, HeLa, and buccal cells; healthy normal cells were devoid of bacteria. Sheared V. parahaemolyticus, i.e., lacking flagella, did not adhere to either normal or degenerating tissue cells. Neither ultraviolet-inactivated organisms nor cell-free culture supernate affected the epithelial cells. Similar findings were observed on the mucosa of the ileum in mice inoculated with V. parahaemolyticus. It appears that V. parahaemolyticus possesses a cytotoxic factor which alters epithelial cells. This factor appears to be closely associated with viable organisms and may be a functional element in the adherence process of flagellated V. parahaemolyticus to mammalian epithelial cells.

scholarly journals Tissue factor–bearing exosome secretion from human mechanically stimulated bronchial epithelial cells in vitro and in vivo

2012 ◽  
Vol 130 (6) ◽  
pp. 1375-1383 ◽  
Author(s):  
Jin-Ah Park ◽  
Asma S. Sharif ◽  
Daniel J. Tschumperlin ◽  
Laurie Lau ◽  
Rachel Limbrey ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tissue Factor ◽  
Bronchial Epithelial Cells ◽  
Bronchial Epithelial
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