scholarly journals Resistance of Melanesian elliptocytes (ovalocytes) to invasion by Plasmodium knowlesi and Plasmodium falciparum malaria parasites in vitro.

1983 ◽  
Vol 71 (3) ◽  
pp. 780-782 ◽  
Author(s):  
T Hadley ◽  
A Saul ◽  
G Lamont ◽  
D E Hudson ◽  
L H Miller ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
Plasmodium Knowlesi ◽  
Plasmodium Falciparum Malaria ◽  
Malaria Parasites

Efficacy of dihydroartemisinin/piperaquine in patients with non-complicated Plasmodium falciparum malaria in Yaoundé, Cameroon

2021 ◽  
Author(s):  
Mélissa Mairet-Khedim ◽  
Sandrine Nsango ◽  
Christelle Ngou ◽  
Sandie Menard ◽  
Camille Roesch ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Southeast Asia ◽  
Falciparum Malaria ◽  
Gene Polymorphisms ◽  
Plasmodium Falciparum Malaria ◽  
Molecular Profile ◽  
Uncomplicated Plasmodium Falciparum Malaria ◽  
In Vitro Survival ◽  
Phenotypic Susceptibility

Abstract Background Dihydroartemisinin/piperaquine is increasingly used for the treatment of uncomplicated Plasmodium falciparum malaria in Africa. The efficacy of this combination in Cameroon is poorly documented, while resistance to dihydroartemisinin/piperaquine readily spreads in Southeast Asia. Objectives This study evaluated the clinical efficacy of dihydroartemisinin/piperaquine in Cameroon, as well as the molecular profile and phenotypic susceptibility of collected isolates to dihydroartemisinin and piperaquine. Patients and methods Dihydroartemisinin/piperaquine efficacy in 42 days was followed-up for 138 patients presenting non-complicated falciparum malaria. Piperaquine concentration was determined at day 7 for 124 patients. kelch13 gene polymorphisms (n = 150) and plasmepsin2 gene amplification (n = 148) were determined as molecular markers of resistance to dihydroartemisinin and piperaquine, respectively. Parasite susceptibility to dihydroartemisinin and piperaquine was determined using validated in vitro survival assays. Results The efficacy of dihydroartemisinin/piperaquine treatment was 100% after PCR correction. The reinfections were not associated with a variation of piperaquine concentration at day 7. Ninety-six percent (144/150) of the samples presented a WT allele of the kelch13 gene. Two percent (3/150) presented the non-synonymous mutation A578S, which is not associated with resistance to dihydroartemisinin. No duplication of the plasmepsin2 gene was observed (0/148). All the samples tested in vitro by survival assays (n = 87) were susceptible to dihydroartemisinin and piperaquine. Conclusions Dihydroartemisinin/piperaquine has demonstrated excellent therapeutic efficacy with no evidence of emerging artemisinin or piperaquine resistance in Yaoundé, Cameroon. This observation suggests that dihydroartemisinin/piperaquine could be a sustainable therapeutic solution for P. falciparum malaria if implemented in areas previously free of artemisinin- and piperaquine-resistant parasites, unlike Southeast Asia.

SHORT REPORT: LACK OF PREDICTION OF AMODIAQUINE EFFICACY IN TREATING PLASMODIUM FALCIPARUM MALARIA BY IN VITRO TESTS

2004 ◽  
Vol 71 (3) ◽  
pp. 294-296 ◽  
Author(s):  
AGNÉS AUBOUY ◽  
ANNICK KEUNDJIAN ◽  
MOHAMED BAKARY ◽  
PHILIPPE DELORON ◽  
JACQUES LE BRAS ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
Plasmodium Falciparum Malaria ◽  
In Vitro Tests ◽  
Short Report

scholarly journals Novel phenotypic assays for the detection of artemisinin-resistant Plasmodium falciparum malaria in Cambodia: in-vitro and ex-vivo drug-response studies

2013 ◽  
Vol 13 (12) ◽  
pp. 1043-1049 ◽  
Author(s):  
Benoit Witkowski ◽  
Chanaki Amaratunga ◽  
Nimol Khim ◽  
Sokunthea Sreng ◽  
Pheaktra Chim ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
Drug Response ◽  
Ex Vivo ◽  
Plasmodium Falciparum Malaria

scholarly journals Zonal human hepatocytes are differentially permissive to Plasmodium falciparum malaria parasites

2021 ◽  
Author(s):  
Annie S P Yang ◽  
Youri M Waardenburg ◽  
Marga Vegte‐Bolmer ◽  
Geert‐Jan A Gemert ◽  
Wouter Graumans ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
Plasmodium Falciparum Malaria ◽  
Human Hepatocytes ◽  
Malaria Parasites
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