scholarly journals Application of the solid phase C1q and Raji cell radioimmune assays for the detection of circulating immune complexes in glomerulonephritis.

1978 ◽  
Vol 62 (1) ◽  
pp. 61-72 ◽  
Author(s):  
K S Tung ◽  
A J Woodroffe ◽  
T D Ahlin ◽  
R C Williams ◽  
C B Wilson
Keyword(s):  
Immune Complexes ◽  
Solid Phase ◽  
Raji Cell ◽  
Circulating Immune Complexes

Circulating Immune Complexes Pre and Post Clotting Factor Infusion in Hemophilia

1981 ◽  
Vol 46 (04) ◽  
pp. 694-698 ◽  
Author(s):  
Edward D Gomperts ◽  
Stanley Jordan ◽  
Debbie Berg ◽  
Rebecca Sakai ◽  
Lois Boylen
Keyword(s):  
Immune Complexes ◽  
Solid Phase ◽  
Raji Cell ◽  
Circulating Immune Complexes ◽  
Blood Type ◽  
Clotting Factor ◽  
Chronic Glomerulonephritis ◽  
Post Infusion ◽  
Factor Concentrate ◽  
Sera Samples

SummaryCirculating immune complexes (CICs) were studied by the Raji cell radioimmune-assay (Raji-RIA) and Clq-solid phase assay (Clq-SPA) in sera samples obtained pre and post replacement infusion from 21 young hemophiliac patients (Mean age 9.5 years). Seven patients had repeat studies. Sixteen patients were selected randomly; the other five were selected as having potential infusion related disease processes. All patients had received multiple infusions prior to entering the study. Five patients were infused with FIX concentrates (2 Hemophilia B, one FVII deficiency, 2 with inhibitors to FVIII). Of the hemophilia A patients (13 severe; 3 mild/moderate), six were infused with ABO-Rh type specific cryoprecipitate and 10 were infused with factor concentrate. Preinfusion sera samples evaluated by the Raji-RIA showed 14 of 28 (50%) sera positive. Eleven of 28 (39.3 %) were positive post infusion in the Raji-RIA. Pre and post infusion studies of the same sera samples using the Clq-SPA showed 5 of 28 (17.9%) positive pre-infusion and 4 of 28(14.3%) positive post infusion. No statistically significant relationship between pre and post infusion studies could be demonstrated.There was no obvious correlation between CIC levels and patient age, race, Epe of infusate, ABO-Rh blood type, hepatosplenomegaly, the presence or absence of HBsAG, antibody, or chronic joint changes. CICs were detected in the three patients with SGOT/SGPT levels greater than 5 times normal, in both patients with chronic renal disease (chronic glomerulonephritis), and two children who had previously demonstrated a Coombs’-positive hemolytic anemia after factor concentrate infusion. Six of eight patients with extremely high levels of CICs had associated clinical or laboratory abnormalities.This data indicated that CICs (as detected by the Raji-RIA) are present in increased frequency in hemophiliac patients. Although individual patients developed CICs post infusion, there is no clinical or statistical data supporting a common infusate immunogen. CICs were present in patients with serum hepatitis and other immune related disorders, probably secondary to factor infusion. The immunopathogenicity and origin of CICs in hemophiliacs is uncertain.

scholarly journals Identification of markers of opportunistic infections in frequently ill children in the North-Eastern regions of Russia

2019 ◽  
Vol 18 (4) ◽  
pp. 5-11 ◽  
Author(s):  
N. V. Karazhas ◽  
L. V. Feklisova ◽  
T. A. Semenenko ◽  
M. N. Kornienko ◽  
T. N. Rybalkina ◽  
...  
Keyword(s):  
Immune Complexes ◽  
Opportunistic Infections ◽  
Solid Phase ◽  
Acute Infection ◽  
Physiological Mechanism ◽  
The Body ◽  
Circulating Immune Complexes ◽  
The North ◽  
North Eastern ◽  
Regions Of Russia

The study is devoted to the clinical examination of frequently ill children in the North-Eastern regions of Russia and the laboratory detection of markers of herpesvirus infections, as well as pneumocystosis and the establishment of the relationship with their immune status.62 children aged 8—15 years were examined. Samples of serum and blood cells, sputum on markers of herpesvirus infections and pneumocystosis by ELISA, indirect immunofluorescence reaction and rapid culture method were studied. Assessment of humoral immunity and determination of circulating immune complexes was performed by solid-phase enzyme immunoassay and immune turbodimetric analysis.The study shows the etiological role of herpesviruses. Active infection (acute, reactivation) was caused in 9.7% of cases of HSV-1,2, 12.9% — EBV, 6.5% — CMV, 19.4% — HHV-6. This is indicated by a large number of convalescents: with EBVI — 40.3%, HSVI — 27.4%, HHVI-6 — 8.1%, CMVI — 4.8%. Also, a significant number of children were found and latent form. The formation of the circulating immune complexes (CIC) is a physiological mechanism of protection of the body, and their excessive accumulation indicates the development of inflammatory processes and autoimmune diseases. In the increase in CIC observed mainly in individuals with latent infection: HSVI — 29.4%; HHVI-6 — 20.8%; CMVI — 16.7%, EBVI — 11.9%. It is important to note that in almost half of the cases the increase in CIC level occurred simultaneously with the increase in the concentration of total IgE. A survey of children on Pneumocystis showed that among them, dominated native — 11.3%, in which the sputum was identified pnevmotsisty. It should be noted that one child was found mixed infection with reactivated CMVI. Acute infection was diagnosed only in 6.4% of the patients.

Circulating Immune Complexes in Human Malignant Melanoma

1982 ◽  
Vol 68 (6) ◽  
pp. 469-472 ◽  
Author(s):  
Raffaele D'Amelio ◽  
Brian Cooke ◽  
John R. Hobbs
Keyword(s):  
Malignant Melanoma ◽  
Immune Complexes ◽  
Solid Phase ◽  
Serum Antibody ◽  
Specific Protein ◽  
Circulating Immune Complexes ◽  
Serum Samples ◽  
Human Malignant Melanoma ◽  
Third Stage ◽  
Solid Phase Assay

The sera from 34 patients with malignant melanoma at various clinical stages of the disease were examined for the presence of circulating immune complexes (CIC) by the C1q solid-phase assay. Their urine and serum samples had been previously examined for the presence of an urinary melanoma-specific protein (MSP) and the corresponding serum antibody. Low levels of CIC (only in the third stage of the disease) and no positive correlation with the presence of MSP were found. The discordance between our and other author's data stresses again the fact that the different laboratory methods for CIC evaluation reveal in a different way the various CIC populations occurring in several diseases.

Detection of circulating immune complexes with a modified raji cell technique

1977 ◽  
Vol 55 (18) ◽  
pp. 899-901 ◽  
Author(s):  
T. H. H�tteroth ◽  
K. H. Meyer zum B�schenfelde
Keyword(s):  
Immune Complexes ◽  
Raji Cell ◽  
Circulating Immune Complexes
Sign in / Sign up

Export Citation Format

Share Document