Protein G ELISA for the Detection of Circulating Immune Complexes. An Alternative to the Solid-Phase Radioimmunoassay for the Monoclonal Rheumatoid Factor

2001 ◽  
Vol 39 (6) ◽  
Author(s):  
Erna Van Hoeveld ◽  
Luc Willebrods ◽  
Xavier Bossuyt
Keyword(s):  
Rheumatoid Factor ◽  
Immune Complexes ◽  
Solid Phase ◽  
Circulating Immune Complexes ◽  
Protein G ◽  
Solid Phase Radioimmunoassay

scholarly journals Identification of markers of opportunistic infections in frequently ill children in the North-Eastern regions of Russia

2019 ◽  
Vol 18 (4) ◽  
pp. 5-11 ◽  
Author(s):  
N. V. Karazhas ◽  
L. V. Feklisova ◽  
T. A. Semenenko ◽  
M. N. Kornienko ◽  
T. N. Rybalkina ◽  
...  
Keyword(s):  
Immune Complexes ◽  
Opportunistic Infections ◽  
Solid Phase ◽  
Acute Infection ◽  
Physiological Mechanism ◽  
The Body ◽  
Circulating Immune Complexes ◽  
The North ◽  
North Eastern ◽  
Regions Of Russia

The study is devoted to the clinical examination of frequently ill children in the North-Eastern regions of Russia and the laboratory detection of markers of herpesvirus infections, as well as pneumocystosis and the establishment of the relationship with their immune status.62 children aged 8—15 years were examined. Samples of serum and blood cells, sputum on markers of herpesvirus infections and pneumocystosis by ELISA, indirect immunofluorescence reaction and rapid culture method were studied. Assessment of humoral immunity and determination of circulating immune complexes was performed by solid-phase enzyme immunoassay and immune turbodimetric analysis.The study shows the etiological role of herpesviruses. Active infection (acute, reactivation) was caused in 9.7% of cases of HSV-1,2, 12.9% — EBV, 6.5% — CMV, 19.4% — HHV-6. This is indicated by a large number of convalescents: with EBVI — 40.3%, HSVI — 27.4%, HHVI-6 — 8.1%, CMVI — 4.8%. Also, a significant number of children were found and latent form. The formation of the circulating immune complexes (CIC) is a physiological mechanism of protection of the body, and their excessive accumulation indicates the development of inflammatory processes and autoimmune diseases. In the increase in CIC observed mainly in individuals with latent infection: HSVI — 29.4%; HHVI-6 — 20.8%; CMVI — 16.7%, EBVI — 11.9%. It is important to note that in almost half of the cases the increase in CIC level occurred simultaneously with the increase in the concentration of total IgE. A survey of children on Pneumocystis showed that among them, dominated native — 11.3%, in which the sputum was identified pnevmotsisty. It should be noted that one child was found mixed infection with reactivated CMVI. Acute infection was diagnosed only in 6.4% of the patients.

Circulating Immune Complexes in Human Malignant Melanoma

1982 ◽  
Vol 68 (6) ◽  
pp. 469-472 ◽  
Author(s):  
Raffaele D'Amelio ◽  
Brian Cooke ◽  
John R. Hobbs
Keyword(s):  
Malignant Melanoma ◽  
Immune Complexes ◽  
Solid Phase ◽  
Serum Antibody ◽  
Specific Protein ◽  
Circulating Immune Complexes ◽  
Serum Samples ◽  
Human Malignant Melanoma ◽  
Third Stage ◽  
Solid Phase Assay

The sera from 34 patients with malignant melanoma at various clinical stages of the disease were examined for the presence of circulating immune complexes (CIC) by the C1q solid-phase assay. Their urine and serum samples had been previously examined for the presence of an urinary melanoma-specific protein (MSP) and the corresponding serum antibody. Low levels of CIC (only in the third stage of the disease) and no positive correlation with the presence of MSP were found. The discordance between our and other author's data stresses again the fact that the different laboratory methods for CIC evaluation reveal in a different way the various CIC populations occurring in several diseases.

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