scholarly journals De novo synthesis of purine nucleotides in human peripheral blood leukocytes. Excessive activity of the pathway in hypoxanthine-guanine phosphoribosyltransferase deficiency.

1976 ◽  
Vol 58 (2) ◽  
pp. 289-297 ◽  
Author(s):  
S Brosh ◽  
P Boer ◽  
B Kupfer ◽  
A de Vries ◽  
O Sperling
Keyword(s):  
Peripheral Blood ◽  
De Novo ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
De Novo Synthesis ◽  
Purine Nucleotides ◽  
Blood Leukocytes ◽  
Hypoxanthine Guanine Phosphoribosyltransferase

De Novo Synthesis of Purine Nucleotides in Human Peripheral Blood Platelets

1977 ◽  
Author(s):  
Z. Jerushalmy ◽  
M. Patya ◽  
O. Sperling
Keyword(s):  
Peripheral Blood ◽  
De Novo ◽  
Human Peripheral Blood ◽  
Blood Platelets ◽  
Calf Serum ◽  
De Novo Synthesis ◽  
Purine Nucleotides ◽  
Detectable Rate ◽  
Normal Human ◽  
Normal Human Peripheral Blood

Human blood platelets were gtudied for the presence of the pathway of de novo synthesis of purine nucleotides. 8 x 108 cells were incubated for 2.5 h at 37°C in 2 ml of Eagle’s Minimal Essential Medium containing Earle’s Balanced Salt Solution, 15% fetal calf serum and 20 μCi sodium [14C] formate (59 mCi/mmole). Platelets were found to incorporate 14C into total purines at a slow but detectable rate of 50–70 pmoles/8 x 108 cells/2.5 h. This incorporation was inhibited by approximately 80% at 10 mM azaserine and by 60% at 0.1 mM adenine. Adenine is known to affect the rate of purine synthesis de novo through the activity of phosphoribosylpyrophosphate amidotransferase, the first committed enzyme of this pathway. The results suggest the presence of the complete pathway of de novo synthesis of purine nucleotides in normal human peripheral blood platelets.

Lactarane Type Sesquiterpenoids as Inhibitors of Leukotriene Biosynthesis and Other, New Metabolites from Submerged Cultures of Lentinellus cochleatus (Pers. ex Fr.) Karst

1996 ◽  
Vol 51 (7-8) ◽  
pp. 493-499 ◽  
Author(s):  
Annette Wunder ◽  
Timm Anke ◽  
Dörte Klostermeyer ◽  
Wolfgang Steglich
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Submerged Cultures ◽  
Blood Leukocytes ◽  
Spectroscopic Investigations ◽  
Basophilic Leukemia ◽  
New Metabolites

Abstract Three known sesquiterpenoids of the lactarane and secolactarane type, deoxylactarorufin A (1), blennin A (2) and blennin C (3), have been obtained from cultures of Lentinellus cochleatus (Basidiomycetes) together with the new metabolites (Z)-2-chloro-3-(4-me-thoxyphenyl)-2-propen-l-ol (4) and lentinellone (5), a protoilludane derivative. The structures were determined by spectroscopic investigations. 1, 2 and 3 are potent inhibitors of leukotriene biosynthesis in rat basophilic leukemia (RBL-1) cells and human peripheral blood leukocytes (PBL).

Translation of interleukin 2 mRNA from human peripheral blood leukocytes in Xenopus oocytes

1982 ◽  
Vol 109 (2) ◽  
pp. 363-369 ◽  
Author(s):  
Shuji Hinuma ◽  
Haruo Onda ◽  
Ken-ichi Naruo ◽  
Yuzo Ichimori ◽  
Masaru Koyama ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Xenopus Oocytes ◽  
Human Peripheral Blood ◽  
Interleukin 2 ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes

Potential anti-inflammatory effects ofMelaleuca alternifolia essential oil on human peripheral blood leukocytes

2006 ◽  
Vol 20 (5) ◽  
pp. 364-370 ◽  
Author(s):  
F. Caldefie-Chézet ◽  
C. Fusillier ◽  
T. Jarde ◽  
H. Laroye ◽  
M. Damez ◽  
...  
Keyword(s):  
Essential Oil ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Anti Inflammatory

scholarly journals Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro

2017 ◽  
Vol 51 (2) ◽  
pp. 39-45
Author(s):  
Milena Jankovic ◽  
Lada Zivkovic ◽  
Andrea Pirkovic ◽  
Dijana Topalovic ◽  
Dragana Dekanski ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Salvianolic Acid B ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Salvianolic Acid

scholarly journals Immune reactions in human filariasis

1978 ◽  
Vol 8 (2) ◽  
pp. 228-232 ◽  
Author(s):  
D Subrahmanyam ◽  
K Mehta ◽  
D S Nelson ◽  
Y V Rao ◽  
C K Rao
Keyword(s):  
Immunoglobulin G ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Wuchereria Bancrofti ◽  
Normal Subjects ◽  
Peripheral Blood Leukocytes ◽  
51Cr Release ◽  
Blood Leukocytes ◽  
Release Studies

Sera from cases of elephantiasis due to Wuchereria bancrofti infection promoted an intense adhesion of peripheral blood leukocytes to W. bancrofti microfilariae in vitro. A similar adhesion was also seen using sera from some normal persons living for several years in areas where filariasis is endemic. No such adhesion was evident with sera from microfilaria carriers or from normal subjects from nonendemic areas. The adhesion was complement independent and was associated with the immunoglobulin G fraction of serum. 51Cr release studies suggested the occurrence of cell-mediated cytotoxicity to W. bancrofti microfilariae in the presence of elephantiasis serum. Microfilariae of Litomosoides carinii could be isolated free of blood cells, from the blood of infected rats. In the presence of serum, or its immunoglobulin G fraction, from patients with elephantiasis, L. carinii microfilariae adhered to human peripheral blood leukocytes or rat spleen cells.

scholarly journals Differential Infectivity and Division ofToxoplasma gondii in Human Peripheral Blood Leukocytes

2000 ◽  
Vol 68 (8) ◽  
pp. 4822-4826 ◽  
Author(s):  
Jacqueline Y. Channon ◽  
Rosanne M. Seguin ◽  
Lloyd H. Kasper
Keyword(s):  
Dendritic Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Cell Types ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Differential Infectivity

ABSTRACT When tachyzoites were incubated with human peripheral blood leukocytes in vitro, more monocytes and dendritic cells than neutrophils or lymphocytes were infected. Although tachyzoites were able to divide in each of these cell types, monocytes and dendritic cells were more permissive to rapid tachyzoite division than neutrophils or lymphocytes.

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