scholarly journals CHARACTERIZATION OF THE PROTEINS OF HUMAN SYNOVIAL FLUID IN CERTAIN DISEASE STATES 1

1956 ◽  
Vol 35 (7) ◽  
pp. 814-824 ◽  
Author(s):  
K. Schmid ◽  
Margaret B. Macnair
Keyword(s):  
Synovial Fluid ◽  
Disease States ◽  
Human Synovial Fluid

scholarly journals Characterization of osteoarthritis phenotypes by global metabolomic profiling of human synovial fluid

2018 ◽  
Author(s):  
Alyssa K. Carlson ◽  
Rachel A. Rawle ◽  
Cameron W. Wallace ◽  
Ellen G. Brooks ◽  
Erik Adams ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Synovial Fluid ◽  
High Performance ◽  
Metabolic Phenotypes ◽  
Disease States ◽  
Structural Deterioration ◽  
Novel Biomarkers ◽  
Central Energy ◽  
Early Oa ◽  
Human Synovial Fluid

AbstractObjectiveOsteoarthritis (OA) is a multifactorial disease with etiological heterogeneity. The objective of this study was to classify OA subgroups by generating metabolic phenotypes of OA from human synovial fluid.DesignPost mortemsynovial fluids (n=75) were analyzed by high performance-liquid chromatography mass spectrometry (HPLC-MS) to measure changes in the global metabolome. Comparisons of healthy (grade 0), early OA (grades I-II), and late OA (grades III-IV) donor populations were considered to reveal phenotypes throughout disease progression.ResultsGlobal metabolomic profiles in synovial fluid were distinct between healthy, early OA, and late OA donors. Pathways differentially activated among these groups included structural deterioration, glycerophospholipid metabolism, inflammation, central energy metabolism, oxidative stress, and vitamin metabolism. Within disease states (early and late OA), subgroups of donors revealed distinct phenotypes. Phenotypes of OA exhibited increased inflammation (early and late OA), oxidative stress (late OA), or structural deterioration (early and late OA) in the synovial fluid.ConclusionThese results revealed distinct metabolic phenotypes of OA in human synovial fluid, provide insight into pathogenesis, represent novel biomarkers and assist in developing personalized interventions for subgroups of OA patients.

scholarly journals First in vivo detection and characterization of hyaluronan-coated extracellular vesicles in human synovial fluid

2016 ◽  
Vol 34 (11) ◽  
pp. 1960-1968 ◽  
Author(s):  
Anne-Mari Mustonen ◽  
Petteri Nieminen ◽  
Antti Joukainen ◽  
Antti Jaroma ◽  
Tommi Kääriäinen ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Extracellular Vesicles ◽  
In Vivo Detection ◽  
Human Synovial Fluid

Capillary electrophoresis of normal human synovial fluid: comparison with fluid from the disease states of osteoarthritis and rheumatoid arthritis

1994 ◽  
Vol 31 (9) ◽  
pp. 257 ◽  
Author(s):  
John M. Duffy ◽  
James Grimshaw ◽  
Aine Kane ◽  
Raymond A. B. Mollan ◽  
Peter L. Spedding ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Capillary Electrophoresis ◽  
Synovial Fluid ◽  
Disease States ◽  
Normal Human ◽  
Human Synovial Fluid

Characterization of human synovial fluid cells of 26 patients with osteoarthritis knee for cartilage repair therapy

2010 ◽  
Vol 13 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Rie KUROSE ◽  
Sadafumi ICHINOHE ◽  
Goro TAJIMA ◽  
Saburo HORIUCHI ◽  
Akira KUROSE ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Cartilage Repair ◽  
Osteoarthritis Knee ◽  
Synovial Fluid Cells ◽  
Human Synovial Fluid

Purification and Characterization of Extracellular Phospholipase A2 from Human Synovial Fluid in Rheumatoid Arthritis1

1989 ◽  
Vol 105 (3) ◽  
pp. 395-399 ◽  
Author(s):  
Shuntaro Hara ◽  
Ichiro Kudo ◽  
Hyuen Wook Chang ◽  
Kunio Matsuta ◽  
Terumasa Miyamoto ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Phospholipase A2 ◽  
Purification And Characterization ◽  
Human Synovial Fluid

scholarly journals Isolation of human synovial-fluid hyaluronate by density-gradient ultracentrifugation and evaluation of its protein content

1972 ◽  
Vol 126 (5) ◽  
pp. 1073-1080 ◽  
Author(s):  
Irwin Scher ◽  
David Hamerman
Keyword(s):  
Synovial Fluid ◽  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Proteolytic Digestion ◽  
Peptide Fragments ◽  
Guanidinium Chloride ◽  
Caesium Chloride ◽  
Normal Human ◽  
Protein Moiety ◽  
Human Synovial Fluid

1. A compound of hyaluronate and protein, called hyaluronate–protein was isolated from pooled human synovial fluids by caesium chloride density-gradient ultracentrifugation. 2. The isolated hyaluronate–protein was labelled with [125I]iodide and the following studies were done. (a) Ultracentrifugation in caesium chloride showed that the protein moiety (125I counts) and hyaluronate (hexuronate) sedimented together in the middle of the gradient. (b) The labelled hyaluronate–protein was treated with trypsin, and ultracentrifugation showed that peptide fragments (125I counts) were dispersed throughout the gradient, indicating proteolytic digestion. Hyaluronate sedimented in the middle of the gradient. (c) The labelled hyaluronate–protein was digested with streptococcal hyaluronidase, and ultracentrifugation showed that hyaluronate fragments were dispersed throughout the gradient, indicating digestion of the polysaccharide. The protein moiety, without attached hyaluronate, now sedimented at the top of the gradient. (d) Ultracentrifugation of labelled hyaluronate–protein in 4m-guanidinium chloride showed that protein and hyaluronate sedimented together. 3. These studies confirm that hyaluronate is combined with a small quantity of protein in normal human synovial fluid. A mild method for the rapid isolation of hyaluronate–protein in good yield is described.

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