A comparison of glycosaminoglycans content of human synovial fluid in different disease states

1984 ◽  
Vol 12 (5) ◽  
pp. 874-874 ◽  
Author(s):  
S. C. MALIK ◽  
J. P. PEARSON ◽  
C. W. DICK
Keyword(s):  
Synovial Fluid ◽  
Disease States ◽  
Human Synovial Fluid

scholarly journals Characterization of osteoarthritis phenotypes by global metabolomic profiling of human synovial fluid

2018 ◽  
Author(s):  
Alyssa K. Carlson ◽  
Rachel A. Rawle ◽  
Cameron W. Wallace ◽  
Ellen G. Brooks ◽  
Erik Adams ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Synovial Fluid ◽  
High Performance ◽  
Metabolic Phenotypes ◽  
Disease States ◽  
Structural Deterioration ◽  
Novel Biomarkers ◽  
Central Energy ◽  
Early Oa ◽  
Human Synovial Fluid

AbstractObjectiveOsteoarthritis (OA) is a multifactorial disease with etiological heterogeneity. The objective of this study was to classify OA subgroups by generating metabolic phenotypes of OA from human synovial fluid.DesignPost mortemsynovial fluids (n=75) were analyzed by high performance-liquid chromatography mass spectrometry (HPLC-MS) to measure changes in the global metabolome. Comparisons of healthy (grade 0), early OA (grades I-II), and late OA (grades III-IV) donor populations were considered to reveal phenotypes throughout disease progression.ResultsGlobal metabolomic profiles in synovial fluid were distinct between healthy, early OA, and late OA donors. Pathways differentially activated among these groups included structural deterioration, glycerophospholipid metabolism, inflammation, central energy metabolism, oxidative stress, and vitamin metabolism. Within disease states (early and late OA), subgroups of donors revealed distinct phenotypes. Phenotypes of OA exhibited increased inflammation (early and late OA), oxidative stress (late OA), or structural deterioration (early and late OA) in the synovial fluid.ConclusionThese results revealed distinct metabolic phenotypes of OA in human synovial fluid, provide insight into pathogenesis, represent novel biomarkers and assist in developing personalized interventions for subgroups of OA patients.

Capillary electrophoresis of normal human synovial fluid: comparison with fluid from the disease states of osteoarthritis and rheumatoid arthritis

1994 ◽  
Vol 31 (9) ◽  
pp. 257 ◽  
Author(s):  
John M. Duffy ◽  
James Grimshaw ◽  
Aine Kane ◽  
Raymond A. B. Mollan ◽  
Peter L. Spedding ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Capillary Electrophoresis ◽  
Synovial Fluid ◽  
Disease States ◽  
Normal Human ◽  
Human Synovial Fluid

scholarly journals Isolation of human synovial-fluid hyaluronate by density-gradient ultracentrifugation and evaluation of its protein content

1972 ◽  
Vol 126 (5) ◽  
pp. 1073-1080 ◽  
Author(s):  
Irwin Scher ◽  
David Hamerman
Keyword(s):  
Synovial Fluid ◽  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Proteolytic Digestion ◽  
Peptide Fragments ◽  
Guanidinium Chloride ◽  
Caesium Chloride ◽  
Normal Human ◽  
Protein Moiety ◽  
Human Synovial Fluid

1. A compound of hyaluronate and protein, called hyaluronate–protein was isolated from pooled human synovial fluids by caesium chloride density-gradient ultracentrifugation. 2. The isolated hyaluronate–protein was labelled with [125I]iodide and the following studies were done. (a) Ultracentrifugation in caesium chloride showed that the protein moiety (125I counts) and hyaluronate (hexuronate) sedimented together in the middle of the gradient. (b) The labelled hyaluronate–protein was treated with trypsin, and ultracentrifugation showed that peptide fragments (125I counts) were dispersed throughout the gradient, indicating proteolytic digestion. Hyaluronate sedimented in the middle of the gradient. (c) The labelled hyaluronate–protein was digested with streptococcal hyaluronidase, and ultracentrifugation showed that hyaluronate fragments were dispersed throughout the gradient, indicating digestion of the polysaccharide. The protein moiety, without attached hyaluronate, now sedimented at the top of the gradient. (d) Ultracentrifugation of labelled hyaluronate–protein in 4m-guanidinium chloride showed that protein and hyaluronate sedimented together. 3. These studies confirm that hyaluronate is combined with a small quantity of protein in normal human synovial fluid. A mild method for the rapid isolation of hyaluronate–protein in good yield is described.

scholarly journals The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells

2017 ◽  
Vol 487 (2) ◽  
pp. 457-463 ◽  
Author(s):  
Farong Ou ◽  
Kai Su ◽  
Jiadong Sun ◽  
Wenting Liao ◽  
Yu Yao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Fluid ◽  
Human Synovial Fluid

scholarly journals SAT0028 The importance of proper handling of human synovial fluid for arthritis research

2018 ◽  
Author(s):  
H. Brouwers ◽  
J.H. von Hegedus ◽  
R.E.M. Toes ◽  
T.W.J. Huizinga ◽  
M.A. Giera ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Human Synovial Fluid

scholarly journals Effect of human synovial fluid in models of in vitro chondrogenic re-differentiation of human primary chondrocytes

2016 ◽  
Vol 24 ◽  
pp. S166
Author(s):  
C. Galeano-Garces ◽  
S.M. Riester ◽  
E.T. Camilleri ◽  
H.S. Ryan ◽  
J. Smith ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Primary Chondrocytes ◽  
Human Primary Chondrocytes ◽  
Human Synovial Fluid

Three-dimensional origami paper-based device for portable immunoassay applications

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 598-607 ◽  
Author(s):  
Chung-An Chen ◽  
Wen-Shin Yeh ◽  
Tsung-Ting Tsai ◽  
Yu-De Li ◽  
Chien-Fu Chen
Keyword(s):  
Staphylococcus Aureus ◽  
Synovial Fluid ◽  
Three Dimensional ◽  
Surface Modified ◽  
Human Synovial Fluid ◽  
Dimensional Surface ◽  
Analytical Device

A three-dimensional surface-modified origami-paper-based analytical device for Staphylococcus aureus detection in highly viscous human synovial fluid.

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