scholarly journals Transcriptomic Profiling of Control and Thyroid-Associated Orbitopathy (TAO) Orbital Fat and TAO Orbital Fibroblasts Undergoing Adipogenesis

2021 ◽  
Vol 62 (9) ◽  
pp. 24
Author(s):  
Dong Won Kim ◽  
Kamil Taneja ◽  
Thanh Hoang ◽  
Clayton P. Santiago ◽  
Timothy J. McCulley ◽  
...  
Keyword(s):  
Transcriptomic Profiling ◽  
Orbital Fat ◽  
Orbital Fibroblasts

scholarly journals Transcriptomic profiling of human orbital fat and differentiating orbital fibroblasts

2021 ◽  
Author(s):  
Dong Won Kim ◽  
Kamil Taneja ◽  
Thanh Hoang ◽  
Clayton Pio Santiago ◽  
Timothy James McCulley ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signaling Pathways ◽  
Time Course ◽  
Principal Component ◽  
Developmental Time ◽  
Rna Seq ◽  
Orbital Fat ◽  
Orbital Fibroblasts ◽  
Compare Gene Expression

Purpose: Orbital fat hyperplasia has a central role in the manifestations of thyroid-associated orbitopathy (TAO). To better understand the pathways involved in adipogenesis in TAO, we have used transcriptomic methods to analyze gene expression in control and TAO patients, as well as in differentiating orbital fibroblasts (OFs). Methods: We performed bulk RNA sequencing (RNA-Seq) on intraconal orbital fat to compare gene expression in control and TAO patients. We treated cultured OFs derived from TAO patients with media containing dexamethasone, insulin, rosiglitazone, and isobutylmethylxanthine (IBMX) to induce adipogenesis. We used single nuclear RNA-Seq (snRNA-Seq) profiling of treated OFs to compare gene expression over time in order to identify pathways that are involved in orbital adipogenesis in vitro and compared the dynamic patterns of gene expression identify differences in gene expression in control and TAO orbital fat. Results: Orbital fat from TAO and control patients segregate with principal component analysis (PCA). Numerous signaling pathways are enriched in orbital fat isolated from TAO patients. SnRNA-Seq of orbital fibroblasts undergoing adipogenesis reveals differential expression of adipocyte-specific genes over the developmental time course. Furthermore, genes that are enriched in TAO orbital fat are also upregulated in orbital adipocytes that differentiate in vitro, while genes that are enriched in control orbital fat are enriched in orbital fibroblasts prior to differentiation. Conclusions: Differentiating orbital fibroblasts serve as a model to study orbital fat hyperplasia seen in TAO. We demonstrate that the insulin-like growth factor-1 receptor (IGF-1R) and Wnt signaling pathways are differentially expressed early in orbital adipogenesis.

Antifibrotic Effects of Quercetin in Primary Orbital Fibroblasts and Orbital Fat Tissue Cultures of Graves' Orbitopathy

2012 ◽  
Vol 53 (9) ◽  
pp. 5921 ◽  
Author(s):  
Jin Sook Yoon ◽  
Min Kyung Chae ◽  
Sun Young Jang ◽  
Sang Yeul Lee ◽  
Eun Jig Lee
Keyword(s):  
Tissue Cultures ◽  
Fat Tissue ◽  
Orbital Fat ◽  
Graves Orbitopathy ◽  
Orbital Fibroblasts

Orbital fat prolapse and dermolipoma: two distinct entities

1994 ◽  
Vol 8 (1) ◽  
pp. 42 ◽  
Author(s):  
Yoon Duck Kim ◽  
Robert A. Goldberg
Keyword(s):  
Orbital Fat

Transcriptomic profiling of canine adrenocortical tumors

2020 ◽  
Author(s):  
Karin Sanders ◽  
Steenbeek Frank Van ◽  
Sara Galac
Keyword(s):  
Transcriptomic Profiling ◽  
Adrenocortical Tumors

RE: Bilateral subconjunctival masses due to orbital fat prolapse

2012 ◽  
Vol 47 (1) ◽  
pp. 81-82 ◽  
Author(s):  
André Jastrzebski ◽  
Seymour Brownstein ◽  
David R. Jordan ◽  
Bruce F. Burns
Keyword(s):  
Orbital Fat

scholarly journals JNK and p38 Inhibitors Prevent Transforming Growth Factor-β1-Induced Myofibroblast Transdifferentiation in Human Graves’ Orbital Fibroblasts

2021 ◽  
Vol 22 (6) ◽  
pp. 2952
Author(s):  
Tzu-Yu Hou ◽  
Shi-Bei Wu ◽  
Hui-Chuan Kau ◽  
Chieh-Chih Tsai
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Mapk Pathway ◽  
Transforming Growth Factor Β1 ◽  
Tissue Growth ◽  
Mitogen Activated Protein Kinase ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Western Blots ◽  
Orbital Fibroblasts ◽  
Tgf Β1

Transforming growth factor-β1 (TGF-β1)-induced myofibroblast transdifferentiation from orbital fibroblasts is known to dominate tissue remodeling and fibrosis in Graves’ ophthalmopathy (GO). However, the signaling pathways through which TGF-β1 activates Graves’ orbital fibroblasts remain unclear. This study investigated the role of the mitogen-activated protein kinase (MAPK) pathway in TGF-β1-induced myofibroblast transdifferentiation in human Graves’ orbital fibroblasts. The MAPK pathway was assessed by measuring the phosphorylation of p38, c-Jun N-terminal kinase (JNK), and extracellular-signal-regulated kinase (ERK) by Western blots. The expression of connective tissue growth factor (CTGF), α-smooth muscle actin (α-SMA), and fibronectin representing fibrogenesis was estimated. The activities of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) responsible for extracellular matrix (ECM) metabolism were analyzed. Specific pharmacologic kinase inhibitors were used to confirm the involvement of the MAPK pathway. After treatment with TGF-β1, the phosphorylation levels of p38 and JNK, but not ERK, were increased. CTGF, α-SMA, and fibronectin, as well as TIMP-1 and TIMP-3, were upregulated, whereas the activities of MMP-2/-9 were inhibited. The effects of TGF-β1 on the expression of these factors were eliminated by p38 and JNK inhibitors. The results suggested that TGF-β1 could induce myofibroblast transdifferentiation in human Graves’ orbital fibroblasts through the p38 and JNK pathways.

scholarly journals Transcriptomic Profiling of Egg Quality in Sea Bass (Dicentrarchus labrax) Sheds Light on Genes Involved in Ubiquitination and Translation

2017 ◽  
Vol 19 (1) ◽  
pp. 102-115 ◽  
Author(s):  
Daniel Żarski ◽  
Thaovi Nguyen ◽  
Aurélie Le Cam ◽  
Jérôme Montfort ◽  
Gilbert Dutto ◽  
...  
Keyword(s):  
Egg Quality ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Transcriptomic Profiling
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