Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at Single-Cell Level

2020 ◽  
Author(s):  
Yuqi Yang ◽  
Fang Guo ◽  
Yue Peng ◽  
Rong Chen ◽  
Wenbai Zhou ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Single Cell ◽  
Human Placenta ◽  
Single Cell Level ◽  
Cell Level ◽  
Transcriptomic Profiling

scholarly journals Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell Level

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuqi Yang ◽  
Fang Guo ◽  
Yue Peng ◽  
Rong Chen ◽  
Wenbo Zhou ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Single Cell ◽  
Human Placenta ◽  
Molecular Mechanisms ◽  
Marker Genes ◽  
Specific Marker ◽  
Increased Risk ◽  
Adverse Pregnancy

Gestational diabetes mellitus (GDM) is associated with an increased risk of adverse pregnancy outcomes. Increasing evidence shows that placentation defects may play important roles in GDM. However, our understanding of the human placenta remains limited. In this study, we generated a comprehensive transcriptomic profile of cellular signatures and transcriptomes in the human placenta in GDM using single-cell RNA sequencing (scRNA-seq), constructed a comprehensive cell atlas, and identified cell subtypes and subtype-specific marker genes. In addition, we investigated the placental cellular function and intercellular interactions in GDM. These findings help to elucidate the molecular mechanisms of GDM, and may facilitate the development of new approaches to GDM treatment and prevention.

scholarly journals Annexin A1 localization and relevance in human placenta from pregnancies complicated by gestational diabetes mellitus. Preliminary results

Placenta ◽  
2016 ◽  
Vol 45 ◽  
pp. 108-109 ◽  
Author(s):  
Juscieli Moreli ◽  
Marina Paula-Silva ◽  
Iracema Calderon ◽  
Sandra Farsky ◽  
Sonia Oliani ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Human Placenta ◽  
Annexin A1 ◽  
Preliminary Results

The metallome of the human placenta in gestational diabetes mellitus

Metallomics ◽  
2015 ◽  
Vol 7 (7) ◽  
pp. 1146-1154 ◽  
Author(s):  
Marco Roverso ◽  
Chiara Berté ◽  
Valerio Di Marco ◽  
Annunziata Lapolla ◽  
Denis Badocco ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Human Placenta

Role of Insulin and Adenosine in the Human Placenta Microvascular and Macrovascular Endothelial Cell Dysfunction in Gestational Diabetes Mellitus

2014 ◽  
Vol 21 (1) ◽  
pp. 26-37 ◽  
Author(s):  
Enrique Guzmán-Gutiérrez ◽  
Pablo Arroyo ◽  
Rocío Salsoso ◽  
Bárbara Fuenzalida ◽  
Tamara Sáez ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Endothelial Cell ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Human Placenta ◽  
Endothelial Cell Dysfunction ◽  
Cell Dysfunction

scholarly journals EP02.07: The metallome of human placenta in gestational diabetes mellitus

2015 ◽  
Vol 46 ◽  
pp. 191-191
Author(s):  
S. Visentin ◽  
A. Lapolla ◽  
V. di Marco ◽  
M. Roverso ◽  
C. Bertè ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Human Placenta

scholarly journals Release and regulation of leptin, resistin and adiponectin from human placenta, fetal membranes, and maternal adipose tissue and skeletal muscle from normal and gestational diabetes mellitus-complicated pregnancies

2005 ◽  
Vol 186 (3) ◽  
pp. 457-465 ◽  
Author(s):  
Martha Lappas ◽  
Kirin Yee ◽  
Michael Permezel ◽  
Gregory E Rice
Keyword(s):  
Diabetes Mellitus ◽  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Pregnant Women ◽  
Human Placenta ◽  
Fetal Membranes ◽  
Maternal Body Mass Index ◽  
Tnf Α

The aim of this study was to determine the release and regulation of leptin, resistin and adiponectin from human placenta and fetal membranes, and maternal subcutaneous adipose tissue and skeletal muscle obtained from normal and gestational diabetes mellitus (GDM)-complicated pregnancies at the time of Cesarean section. Tissue explants were incubated in the absence (basal control) or presence of 10 μg/ml lipopolysaccharide (LPS), 10, 20 or 40 ng/ml tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and IL-8, 1 μM phorbol myristate acetate, 10, 20 and 40 mM glucose, 0.1, 1 and 10 μM insulin and 0.1 1 and 10 μM dexamethasone, progesterone and estrogen. After an 18-h incubation, the medium was collected and the release of leptin, resistin and adiponectin was quantified by ELISA. Human gestational tissues and maternal tissues released immunoreactive leptin, resistin and adiponectin; however, there was no difference in the release of either resistin or adiponectin between normal pregnant women and women with gestational diabetes. The release of leptin was significantly higher in placenta, amnion and choriodecidua obtained from normal pregnant women compared with women with GDM. However, in maternal tissues, the situation was reversed, with adipose tissue and skeletal muscle obtained from women with GDM releasing significantly greater amounts of leptin. In adipose tissue and skeletal muscle the release of leptin was significantly greater in insulin-controlled GDM compared with diet-controlled GDM, and leptin release from adipose tissue was significantly correlated with maternal body mass index. In all tissues tested, there was no effect of incubation with LPS, IL-6, IL-8 or TNF-α on leptin, resistin or adiponectin release. PMA significantly increased the release of resistin from placenta and adipose tissue. Insulin increased placental resistin release, whereas the hormones dexamethasone, progesterone and estrogen significantly decreased placental resistin release. The data presented in this study demonstrate that dysregulation of leptin metabolism and/or function in the placenta may be implicated in the pathogenesis of GDM. Furthermore, resistin release is greatly affected by a variety of inflammatory mediators and hormones.

Effects of gestational diabetes mellitus on proteins implicated in insulin signaling in human placenta

2006 ◽  
Vol 22 (9) ◽  
pp. 526-535 ◽  
Author(s):  
Ana Alonso ◽  
Carmen González Del Rey ◽  
Ana Navarro ◽  
Jorge Tolivia ◽  
Celestino G. González
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Human Placenta ◽  
Insulin Signaling

scholarly journals Elevated Soluble CD163 in Gestational Diabetes Mellitus: Secretion from Human Placenta and Adipose Tissue

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e101327 ◽  
Author(s):  
Muhammad Furqan Bari ◽  
Martin O. Weickert ◽  
Kavitha Sivakumar ◽  
Sean G. James ◽  
David R. J. Snead ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Adipose Tissue ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Human Placenta ◽  
Soluble Cd163
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