scholarly journals Novel Insights Into Muscarinic and Purinergic Responses in Primary Cultures of Rat Lacrimal Gland Myoepithelial Cells

2021 ◽  
Vol 62 (12) ◽  
pp. 19
Author(s):  
Martin Dankis ◽  
Thomas Carlsson ◽  
Patrik Aronsson ◽  
Gunnar Tobin ◽  
Michael Winder
Keyword(s):  
Lacrimal Gland ◽  
Primary Cultures ◽  
Myoepithelial Cells

scholarly journals Lacrimal Gland Myoepithelial Cells Are Altered in a Mouse Model of Dry Eye Disease

2020 ◽  
Vol 190 (10) ◽  
pp. 2067-2079 ◽  
Author(s):  
Laura García-Posadas ◽  
Robin R. Hodges ◽  
Tor P. Utheim ◽  
Ole Kristoffer Olstad ◽  
Vanessa Delcroix ◽  
...  
Keyword(s):  
Mouse Model ◽  
Dry Eye ◽  
Lacrimal Gland ◽  
Myoepithelial Cells ◽  
Dry Eye Disease ◽  
Eye Disease

scholarly journals Pleomorphic Adenoma of Lacrimal Gland: A Rare Entity

2021 ◽  
Vol 8 (7) ◽  
pp. C100-104
Author(s):  
Kirti Chadha Kazi ◽  
Vivek Choodamani Parameshwar ◽  
Vikas S Kavishwar ◽  
Prithesh Bhaskar Shetty
Keyword(s):  
Pleomorphic Adenoma ◽  
Lacrimal Gland ◽  
Histopathological Examination ◽  
Mass Effect ◽  
Final Diagnosis ◽  
Myoepithelial Cells ◽  
Significant Loss ◽  
Excision Biopsy ◽  
Post Surgery ◽  
Rare Tumours

Among the various tumours affecting the lacrimal gland, pleomorphic adenomas though most common can present clinically and radiologically as worrisome tumours. Histopathologic diagnosis hence is very critical for these tumours. We present a case of pleomorphic adenoma of the lacrimal gland which we encountered at our institutes. The patient was a 46-year-old male who came with complaints of a gradually progressing swelling in the right eye not associated with much pain or significant loss of vision. MRI revealed a large lobulated extrachoanal mass causing mass effect and scalloping of the adjacent bony structures. An excision biopsy was done and sent for histopathological examination. Histologically the tumour showed a biphasic morphology comprising of epithelial (ductal and myoepithelial) and chondromyxoid stromal components. Immunohistochemistry showed positivity for pan CK and CK 7 in ductal epithelial cells, p63 and S100 in myoepithelial cells and a low Ki67 index. On the basis of immunohistochemistry profile along with clinicoradiological correlation a final diagnosis of pleomorphic adenoma of the lacrimal gland was made. The patient post-surgery was doing well but was lost to follow up. It can thus be seen that is it absolutely necessary to accurately diagnose these benign but clinically misconceiving rare tumours of the lacrimal gland for appropriate management of such patients.

Gene Delivery by Viral Vectors in Primary Cultures of Lacrimal Gland Tissue

2003 ◽  
Vol 44 (4) ◽  
pp. 1529 ◽  
Author(s):  
Eyal Banin ◽  
Alexey Obolensky ◽  
Elena Piontek ◽  
Haya Falk ◽  
Eli Pikarsky ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Lacrimal Gland ◽  
Viral Vectors ◽  
Primary Cultures ◽  
Gland Tissue

scholarly journals Direct interaction between Rab3D and the polymeric immunoglobulin receptor and trafficking through regulated secretory vesicles in lacrimal gland acinar cells

2008 ◽  
Vol 294 (3) ◽  
pp. C662-C674 ◽  
Author(s):  
Eunbyul Evans ◽  
Wenzheng Zhang ◽  
Galina Jerdeva ◽  
Chiao-Yu Chen ◽  
Xuequn Chen ◽  
...  
Keyword(s):  
Lacrimal Gland ◽  
Secretory Pathway ◽  
Primary Cultures ◽  
Direct Interaction ◽  
Membrane Vesicles ◽  
Acinar Cells ◽  
Small Gtpase ◽  
Secretory Proteins ◽  
Major Protein ◽  
Resting Cells

The lacrimal gland is responsible for tear production, and a major protein found in tears is secretory component (SC), the proteolytically cleaved fragment of the extracellular domain of the polymeric Ig receptor (pIgR), which is the receptor mediating the basal-to-apical transcytosis of polymeric immunoglobulins across epithelial cells. Immunofluorescent labeling of rabbit lacrimal gland acinar cells (LGACs) revealed that the small GTPase Rab3D, a regulated secretory vesicle marker, and the pIgR are colocalized in subapical membrane vesicles. In addition, the secretion of SC from primary cultures of LGACs was stimulated by the cholinergic agonist carbachol (CCH), and its release rate was very similar to that of other regulated secretory proteins in LGACs. In pull-down assays from resting LGACs, recombinant wild-type Rab3D (Rab3DWT) or the GDP-locked mutant Rab3DT36N both pulled down pIgR, but the GTP-locked mutant Rab3DQ81L did not. When the pull-down assays were performed in the presence of guanosine-5′-(γ-thio)-triphosphate, GTP, or guanosine-5′- O-(2-thiodiphosphate), binding of Rab3DWT to pIgR was inhibited. In blot overlays, recombinant Rab3DWT bound to immunoprecipitated pIgR, suggesting that Rab3D and pIgR may interact directly. Adenovirus-mediated overexpression of mutant Rab3DT36N in LGACs inhibited CCH-stimulated SC release, and, in CCH-stimulated LGACs, pull down of pIgR with Rab3DWT and colocalization of pIgR with endogenous Rab3D were decreased relative to resting cells, suggesting that the pIgR-Rab3D interaction may be modulated by secretagogues. These data suggest that the novel localization of pIgR to the regulated secretory pathway of LGACs and its secretion therefrom may be affected by its novel interaction with Rab3D.

scholarly journals Aquaporins 8 and 9 as Possible Markers for Adult Murine Lacrimal Gland Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Naoko Okada ◽  
Tetsuya Kawakita ◽  
Masataka Ito ◽  
Kazuo Tsubota
Keyword(s):  
Cell Membrane ◽  
Expression Pattern ◽  
Lacrimal Gland ◽  
Family Members ◽  
Myoepithelial Cells ◽  
Lacrimal Glands ◽  
Total Rna ◽  
Gland Cells ◽  
Murine Embryos ◽  
Acinar Unit

Aquaporins (AQPs) are proteins that selectively transport water across the cell membrane. Although AQPs play important roles in secretion in the lacrimal gland, the expression and localization of AQPs have not been clarified yet. In the current study, we investigated the expression pattern of AQP family members in the murine lacrimal gland during development. Lacrimal gland tissues were harvested from E13.5 and E17.5 murine embryos and from mice 8 weeks of age (adults). Corneal and conjunctival tissues from the latter served as controls. Total RNA was isolated and analyzed for the expression of AQP family members using qPCR. The localization of AQPs in the adult lacrimal gland in adult murine lacrimal glands was also analyzed. Expression of Aqp8 and Aqp9 mRNAs was detected in the adult lacrimal gland but not in the cornea, conjunctiva, or fetal lacrimal gland. AQP8 and AQP9 and α-SMA partially colocalized around the basal regions of the acinar unit. The levels of Aqp3 mRNAs and protein were much lower in the adult lacrimal gland but were readily detected in the adult cornea and conjunctiva. Our study suggests that AQP8 and AQP9 may serve as markers for adult murine lacrimal gland, ductal, and myoepithelial cells.

scholarly journals Lacrimal gland adenoma in a sheep

2019 ◽  
Vol 22 (1) ◽  
pp. 122-126
Author(s):  
G. Farjanikish ◽  
A. Khodakaram-Tafti ◽  
M. Ghane
Keyword(s):  
Epithelial Cells ◽  
Lacrimal Gland ◽  
Corneal Edema ◽  
Cell Types ◽  
Myoepithelial Cells ◽  
Tubular Structures ◽  
Mitotic Figures ◽  
Veterinary Hospital ◽  
Cellular Pleomorphism ◽  
Luminal Epithelial Cells

The lacrimal gland is a diamond-shaped, tubuloalveolar gland that secretes the serous component of tears. A four-year-old female crossbreed sheep suffering from left eye protrusion was referred to a Veterinary Hospital. Ophthalmic examination revealed epiphora, superficial ulcerative keratitis, corneal edema and neovascularisation. Moreover, ultrasound examination showed a large heterogeneous mass with variable reflectivity in the intraconal and extraconal spaces. Grossly, a 2.5×1.5×0.5 cm oval firm grayish mass was observed. Histopathologically, the mass was composed mainly by tubules with two cell types including cuboidal luminal epithelial cells and peripheral myoepithelial cells. The tubular structures were separated by proliferating myoepithelial cells. Mitotic figures, cellular pleomorphism and atypia were not seen. Immunohistochemically, most of the luminal epithelial cells showed an immunopositive reaction with a cytokeratin (AE1/AE3) marker. On the basis of these findings, the mass was diagnosed as a lacrimal gland adenoma.

scholarly journals Role of the Phospholipase C Pathway and Calcium Mobilization in Oxytocin-Induced Contraction of Lacrimal Gland Myoepithelial Cells

2021 ◽  
Vol 62 (14) ◽  
pp. 25
Author(s):  
Angela Gárriz ◽  
Salome Aubry ◽  
Quentin Wattiaux ◽  
Jeffrey Bair ◽  
Michael Mariano ◽  
...  
Keyword(s):  
Phospholipase C ◽  
Lacrimal Gland ◽  
Myoepithelial Cells ◽  
Calcium Mobilization

Myoepithelial Cells and Neuronal Nitric Oxide Synthase in the Rabbit Lacrimal Gland

2005 ◽  
Vol 3 ◽  
pp. S57 ◽  
Author(s):  
Chuanqing Ding ◽  
Jessica Wong ◽  
Austin K. Mircheff ◽  
Joel E. Schechter
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Lacrimal Gland ◽  
Neuronal Nitric Oxide Synthase ◽  
Myoepithelial Cells ◽  
Oxide Synthase
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