scholarly journals Complement-Mediated Regulation of Apolipoprotein E in Cultured Human RPE Cells

2017 ◽  
Vol 58 (7) ◽  
pp. 3073 ◽  
Author(s):  
Ping Yang ◽  
Nikolai P. Skiba ◽  
Grace M. Tewkesbury ◽  
Victoria M. Treboschi ◽  
Peter Baciu ◽  
...  
Keyword(s):  
Apolipoprotein E ◽  
Rpe Cells ◽  
Human Rpe Cells

Complement-mediated release of fibroblast growth factor 2 from human RPE cells

2021 ◽  
Vol 204 ◽  
pp. 108471
Author(s):  
Ping Yang ◽  
Samantha E. Neal ◽  
Kristen L. Buehne ◽  
Grace M. Tewkesbury ◽  
Mikael Klingeborn ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Rpe Cells ◽  
Human Rpe Cells

Diphenyleneiodonium induces ROS-independent p53 expression and apoptosis in human RPE cells

FEBS Letters ◽  
2006 ◽  
Vol 581 (2) ◽  
pp. 180-186 ◽  
Author(s):  
Sang Eun Park ◽  
Ju Dong Song ◽  
Kang Mi Kim ◽  
Yeong Min Park ◽  
Nam Deuk Kim ◽  
...  
Keyword(s):  
P53 Expression ◽  
Rpe Cells ◽  
Human Rpe Cells

Effect of NF-κB Inhibition on TNF-α–Induced Apoptosis in Human RPE Cells

2004 ◽  
Vol 45 (7) ◽  
pp. 2438 ◽  
Author(s):  
Ping Yang ◽  
Brian S. McKay ◽  
Janice B. Allen ◽  
Glenn J. Jaffe
Keyword(s):  
Rpe Cells ◽  
Tnf Α ◽  
Induced Apoptosis ◽  
Human Rpe Cells

Transplantation of Retinal Pigment Epithelium Using Viable Cryopreserved Cells

1997 ◽  
Vol 6 (2) ◽  
pp. 149-162 ◽  
Author(s):  
Yusuf K. Durlu ◽  
Makoto Tamai
Keyword(s):  
Tissue Culture ◽  
Indirect Elisa ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Light And Electron Microscopy ◽  
Blue Dye ◽  
Rpe Cells ◽  
Before And After ◽  
Rcs Rats ◽  
Human Rpe Cells

Transplantation of retinal pigment epithelium (RPE) may have potential clinical application for the surgical treatment of RPE-specific retinal degeneration, including age-related macular degeneration. The feasibility of an RPE storage bank has been investigated by experimenting with transplantation using viable, cryopreserved RPE cells. Fresh and cultured fetal human and bovine RPE cells were cryopreserved in 90% fetal bovine serum containing 10% dimethyl sulfoxide. The viability of the cells before and after cryopreservation was evaluated by trypan blue dye exclusion test, microculture tetrazolium assay (MTA), tissue culture, and transplantation after cryopreservation. The origin of RPE cells before and after cryopreservation was assessed by immunocytochemistry, immunoblotting, and indirect ELISA of RPE-marker protein using cytokeratin for cultured fetal human RPE cells and by immunocytochemistry of cellular retinaldehyde-binding protein (CR-ALBP) for cultured bovine RPE cells. Freshly isolated and cryopreserved uncultured bovine RPE cells were transplanted by posterior transscleral approach into the subretinal spaces of adult albino rabbits and 23-day-old Royal College of Surgeons (RCS) rats with a 33 gauge Hamilton syringe. Following surgery, artificial retinal blebs were confirmed by fundus examination. Morphologic examination was performed postoperatively by light and electron microscopy in albino rabbits and by light microscopy in RCS rats up to 3 mo. Control subretinal injections using vehicle solution also were performed in RCS rats. Cultured fetal human and bovine RPE cells after cryopreservation were found to be viable, based on the results of trypan blue dye exclusion test, MTA, tissue culture, and transplantation. Expression and reexpression of cytokeratin intermediate filaments in cultured fetal human RPE were demonstrated by immunocytochemistry, immunoblotting, and indirect ELISA before and after cryopreservation. Immunocytochemistry of CRALBP before and after cryopreservation in uncultured bovine RPE cells disclosed expression and reexpression of RPE cell marker protein. No uncultured fetal human RPE cells showed proliferation in tissue culture after cryopreservation. In rabbits, light and electron microscopy disclosed xenografted RPE cells residing on Bruch's membrane of the host retina. No sign of graft vs. host reaction was observed. No morphologic difference was noted between the fresh and 10-day-old cryopreserved RPE cells in situ following transplantation at day 25. In RCS rats, subretinal injection of 3-wk-old cryopreserved bovine RPE cells partially rescued photoreceptor cells locally at the transplanted area observed at 3 mo postoperatively. The retinal photoreceptors at the inferior hemisphere of the transplanted eye and the eye injected with vehicle solution showed no rescue effect. We found that cryopreserved cultured fetal human RPE cells and uncultured and cultured bovine RPE cells can be used for RPE transplantation studies. The ability to create an RPE storage bank as a source of donor cells may result in several clinical advantages.

Effect of Endothelin-1 on proliferation, migration and fibrogenic gene expression in human RPE cells

Peptides ◽  
2017 ◽  
Vol 94 ◽  
pp. 43-48 ◽  
Author(s):  
Dong Qin ◽  
Li Zhang ◽  
Xuemin Jin ◽  
Zhaoxia Zhao ◽  
Yanrong Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rpe Cells ◽  
Endothelin 1 ◽  
Human Rpe Cells

Inhibition of VEGF Expression by Targeting HIF-1α with Small Interference RNA in Human RPE Cells

2007 ◽  
Vol 221 (6) ◽  
pp. 411-417 ◽  
Author(s):  
Peng Zhang ◽  
Yusheng Wang ◽  
Yannian Hui ◽  
Dan Hu ◽  
Haiyan Wang ◽  
...  
Keyword(s):  
Vegf Expression ◽  
Small Interference Rna ◽  
Rpe Cells ◽  
Interference Rna ◽  
Human Rpe Cells

scholarly journals Preservation of outer retina and its synaptic connectivity following subretinal injections of human RPE cells in the Royal College of Surgeons rat

2007 ◽  
Vol 85 (3) ◽  
pp. 381-392 ◽  
Author(s):  
Isabel Pinilla ◽  
Nicolás Cuenca ◽  
Yves Sauvé ◽  
Shaomei Wang ◽  
Raymond D. Lund
Keyword(s):  
Royal College ◽  
Synaptic Connectivity ◽  
Outer Retina ◽  
Rpe Cells ◽  
Human Rpe Cells
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