scholarly journals In Vivo Two-Photon Fluorescence Kinetics of Primate Rods and Cones

2016 ◽  
Vol 57 (2) ◽  
pp. 647 ◽  
Author(s):  
Robin Sharma ◽  
Christina Schwarz ◽  
David R. Williams ◽  
Grazyna Palczewska ◽  
Krzysztof Palczewski ◽  
...  
Keyword(s):  
Fluorescence Kinetics ◽  
Rods And Cones ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Kinetics Of

Silole-Based Red Fluorescent Organic Dots for Bright Two-Photon Fluorescence In vitro Cell and In vivo Blood Vessel Imaging

Small ◽  
2015 ◽  
Vol 12 (6) ◽  
pp. 782-792 ◽  
Author(s):  
Bin Chen ◽  
Guangxue Feng ◽  
Bairong He ◽  
Chiching Goh ◽  
Shidang Xu ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Two Photon ◽  
Vessel Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Two-Photon Microscopy Allows Imaging and Characterization of Cochlear Microvasculature In Vivo

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Friedrich Ihler ◽  
Mattis Bertlich ◽  
Bernhard Weiss ◽  
Steffen Dietzel ◽  
Martin Canis
Keyword(s):  
Fluorescence Microscopy ◽  
Inner Ear ◽  
Ex Vivo ◽  
Small Scale ◽  
Published Data ◽  
Cochlear Blood Flow ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Impairment of cochlear blood flow has been discussed as factor in the pathophysiology of various inner ear disorders. However, the microscopic study of cochlear microcirculation is limited due to small scale and anatomical constraints. Here, two-photon fluorescence microscopy is applied to visualize cochlear microvessels. Guinea pigs were injected with Fluorescein isothiocyanate- or Texas red-dextrane as plasma marker. Intravital microscopy was performed in four animals and explanted cochleae from four animals were studied. The vascular architecture of the cochlea was visualized up to a depth of90.0±22.7 μm. Imaging yielded a mean contrast-to-noise ratio (CNR) of3.3±1.7. Mean diameter in vivo was16.5±6.0 μm for arterioles and8.0±2.4 μm for capillaries. In explanted cochleae, the diameter of radiating arterioles and capillaries was measured with12.2±1.6 μm and6.6±1.0 μm, respectively. The difference between capillaries and arterioles was statistically significant in both experimental setups (P<0.001andP=0.022, two-way ANOVA). Measured vessel diameters in vivo and ex vivo were in agreement with published data. We conclude that two-photon fluorescence microscopy allows the investigation of cochlear microvessels and is potentially a valuable tool for inner ear research.

Unprecedentedly High Tissue Penetration Capability of Co-Assembled Nanosystems for Two-Photon Fluorescence Imaging In Vivo

2015 ◽  
Vol 3 (5) ◽  
pp. 646-651 ◽  
Author(s):  
Pei-Pei Yang ◽  
Yang Yang ◽  
Yu-Juan Gao ◽  
Yi Wang ◽  
Ju-Chen Zhang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Tissue Penetration ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A two-photon fluorescent probe for basal formaldehyde imaging in zebrafish and visualization of mitochondrial damage induced by FA stress

The Analyst ◽  
2019 ◽  
Vol 144 (7) ◽  
pp. 2297-2303 ◽  
Author(s):  
Fangyun Xin ◽  
Yong Tian ◽  
Congcong Gao ◽  
Bingpeng Guo ◽  
Yulong Wu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Probe ◽  
Mitochondrial Damage ◽  
Mitochondrial Morphology ◽  
Morphology Change ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A two-photon fluorescence probe Mito-FA-FP can monitor mitochondrial morphology change and image endogenous FA in vivo.

Color‐Tunable Light‐up Bioorthogonal Probes for In Vivo Two‐Photon Fluorescence Imaging

2020 ◽  
Vol 26 (20) ◽  
pp. 4576-4582 ◽  
Author(s):  
Yandong Dou ◽  
Yajun Wang ◽  
Yukun Duan ◽  
Bin Liu ◽  
Qinglian Hu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Two Photon ◽  
Color Tunable ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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