Reliability of the Mouse Model of Choroidal Neovascularization Induced by Laser Photocoagulation

2014 ◽  
Vol 55 (10) ◽  
pp. 6525 ◽  
Author(s):  
Stephen H. Poor ◽  
Yubin Qiu ◽  
Elizabeth S. Fassbender ◽  
Siyuan Shen ◽  
Amber Woolfenden ◽  
...  
Keyword(s):  
Mouse Model ◽  
Choroidal Neovascularization ◽  
Laser Photocoagulation

scholarly journals Cytokine Profiles and the Effect of Intravitreal Aflibercept Treatment on Experimental Choroidal Neovascularization

2020 ◽  
Author(s):  
Chen Wang ◽  
Rongrong Zhang ◽  
Qi Zhang ◽  
Huixiang Jin ◽  
Chenghua Wei ◽  
...  
Keyword(s):  
Mouse Model ◽  
Choroidal Neovascularization ◽  
Inflammatory Cytokines ◽  
Laser Photocoagulation ◽  
Macrophage Polarization ◽  
Fold Change ◽  
Lesion Area ◽  
M2 Polarization ◽  
Intravitreal Aflibercept ◽  
Fas L

Purpose: The purpose of our study was to investigate the profiles of inflammatory cytokines and the macrophage polarization gene in a choroidal neovascularization (CNV) mouse model before and after intravitreal aflibercept treatment. Methods: The CNV mouse model was conducted by laser photocoagulation. A total of 58 cytokines were measured by multiplex mouse cytokine antibody array. The macrophage polarization genes were tested by reverse transcription polymerase chain reaction. The relationship between the cytokines and the CNV lesion area was analyzed by correlation. Results: MIP-1a on day 3 after laser photocoagulation, MCP-5 and Fas-L on day 7, and IL-15 and IL-7 on day 14 were significantly upregulated (p< 0.001, fold change > 10.0). After the intravitreal aflibercept treatment, GM-CSF and MCP-1 on day 3 and TIMP-1 on days 7 and 14 were the most significantly upregulated cytokines (p< 0.001, fold change > 10.0). MIP-1 on day 3, IL-13 and Fas-L on day 7, and Fas-L on day 14 were the most significantly downregulated cytokines after intravitreal aflibercept treatment (p< 0.001, fold change > 5.0). M2 polarization and VEGFA genes were significantly increased in the CNV formation, whereas aflibercept suppressed M2 polarization and VEGFA genes. IL-7 was negatively related to the CNV lesion area on day 14 after intravitreal aflibercept treatment (r = −0.938, p = 0.006). Conclusion: The inflammatory cytokines and the M1/M2 macrophage genes significantly changed in the CNV mouse model. This result suggests that inflammatory cytokines and macrophages play a critical role in the physiopathology of CNV.

Transpupillary Thermotherapy of Juxtafoveal Recurrent Choroidal Neovascularization

2003 ◽  
Vol 13 (5) ◽  
pp. 453-460 ◽  
Author(s):  
F. Cardillo Piccolino ◽  
C.M. Eandi ◽  
L. Ventre ◽  
R.C. Rigault De La Longrais ◽  
F.M. Grignolo
Keyword(s):  
Low Power ◽  
Choroidal Neovascularization ◽  
Laser Photocoagulation ◽  
Age Related Macular Degeneration ◽  
Retinal Damage ◽  
Transpupillary Thermotherapy ◽  
Age Related ◽  
Minute Duration ◽  
Conventional Laser

Purpose To evaluate the effectiveness of low power transpupillary thermotherapy (TTT) in treating juxtafoveal recurrent choroidal neovascularization (CNV) after laser photocoagulation in patients with age-related macular degeneration (ARMD). Methods Eight eyes of eight patients with ARMD and juxtafoveal recurrent CNV were treated with low power TTT, delivered using an 810-nm diode laser with 350 mW, 2.0 mm spot, and 1-minute duration. Visual acuity (VA) ranged from 20/100 to 20/50. Treatment effect was evaluated by fluorescein angiography, indocyanine green angiography, and VA measurements (Early Treatment Diabetic Retinopathy Study) at 1-week, 2-week, and monthly follow-up visits. Results NO retinal damage was visible ophthalmoscopically during treatment. At the first follow-up visit, seven eyes had obliteration of CNV and one eye required a second TTT application. VA was unchanged in six eyes, improved in one eye, and worsened in one eye. Recurrences occurred in all eyes between 1 and 7 months after TTT and were treated with photodynamic therapy (PDT). More than two PDT treatments were performed in each eye in the year after recurrence. Conclusions LOW power TTT is as able to close juxtafoveal recurrent CNV as is high power conventional laser photocoagulation but does not prevent recurrences. Further intervention with TTT in order to treat recurrences is under investigation.

scholarly journals The Histone Deacetylase Inhibitor AN7, Attenuates Choroidal Neovascularization in a Mouse Model

2019 ◽  
Vol 20 (3) ◽  
pp. 714
Author(s):  
Mor Dahbash ◽  
Ruti Sella ◽  
Elinor Megiddo-Barnir ◽  
Yael Nisgav ◽  
Nataly Tarasenko ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mouse Model ◽  
Histone Deacetylase ◽  
Choroidal Neovascularization ◽  
Histone Deacetylase Inhibitor ◽  
Vision Loss ◽  
Age Related Macular Degeneration ◽  
Control Group ◽  
Age Related ◽  
Deacetylase Inhibitor

: Choroidal neovascularization (CNV) is a complication of age-related macular degeneration and a major contributing factor to vision loss. In this paper, we show that in a mouse model of laser-induced CNV, systemic administration of Butyroyloxymethyl-diethyl phosphate (AN7), a histone deacetylase inhibitor (HDACi), significantly reduced CNV area and vascular leakage, as measured by choroidal flatmounts and fluorescein angiography. CNV area reduction by systemic AN7 treatment was similar to that achieved by intravitreal bevacizumab treatment. The expression of vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF-2), and the endothelial cells marker CD31, was lower in the AN7 treated group in comparison to the control group at the laser lesion site. In vitro, AN7 facilitated retinal pigmented epithelium (RPE) cells tight junctions’ integrity during hypoxia, by protecting the hexagonal pattern of ZO-1 protein in the cell borders, hence reducing RPE permeability. In conclusion, systemic AN7 should be further investigated as a possible effective treatment for CNV.

Type I pig collagen enhances the efficacy of PEDF 34-mer peptide in a mouse model of laser-induced choroidal neovascularization

2019 ◽  
Vol 257 (8) ◽  
pp. 1709-1717 ◽  
Author(s):  
Hyun Woong Kim ◽  
Kug-Hwan Roh ◽  
Seong Wook Kim ◽  
Sung Jae Park ◽  
Na-Young Lim ◽  
...  
Keyword(s):  
Mouse Model ◽  
Choroidal Neovascularization ◽  
Type I

scholarly journals A Mouse Model for Laser-induced Choroidal Neovascularization

10.3791/53502 ◽  
2015 ◽  
Author(s):  
Ronil S. Shah ◽  
Brian T. Soetikno ◽  
Michelle Lajko ◽  
Amani A. Fawzi
Keyword(s):  
Mouse Model ◽  
Choroidal Neovascularization
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