scholarly journals A Mouse Model for Laser-induced Choroidal Neovascularization

10.3791/53502 ◽  
2015 ◽  
Author(s):  
Ronil S. Shah ◽  
Brian T. Soetikno ◽  
Michelle Lajko ◽  
Amani A. Fawzi
Keyword(s):  
Mouse Model ◽  
Choroidal Neovascularization

scholarly journals The Histone Deacetylase Inhibitor AN7, Attenuates Choroidal Neovascularization in a Mouse Model

2019 ◽  
Vol 20 (3) ◽  
pp. 714
Author(s):  
Mor Dahbash ◽  
Ruti Sella ◽  
Elinor Megiddo-Barnir ◽  
Yael Nisgav ◽  
Nataly Tarasenko ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mouse Model ◽  
Histone Deacetylase ◽  
Choroidal Neovascularization ◽  
Histone Deacetylase Inhibitor ◽  
Vision Loss ◽  
Age Related Macular Degeneration ◽  
Control Group ◽  
Age Related ◽  
Deacetylase Inhibitor

: Choroidal neovascularization (CNV) is a complication of age-related macular degeneration and a major contributing factor to vision loss. In this paper, we show that in a mouse model of laser-induced CNV, systemic administration of Butyroyloxymethyl-diethyl phosphate (AN7), a histone deacetylase inhibitor (HDACi), significantly reduced CNV area and vascular leakage, as measured by choroidal flatmounts and fluorescein angiography. CNV area reduction by systemic AN7 treatment was similar to that achieved by intravitreal bevacizumab treatment. The expression of vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF-2), and the endothelial cells marker CD31, was lower in the AN7 treated group in comparison to the control group at the laser lesion site. In vitro, AN7 facilitated retinal pigmented epithelium (RPE) cells tight junctions’ integrity during hypoxia, by protecting the hexagonal pattern of ZO-1 protein in the cell borders, hence reducing RPE permeability. In conclusion, systemic AN7 should be further investigated as a possible effective treatment for CNV.

Type I pig collagen enhances the efficacy of PEDF 34-mer peptide in a mouse model of laser-induced choroidal neovascularization

2019 ◽  
Vol 257 (8) ◽  
pp. 1709-1717 ◽  
Author(s):  
Hyun Woong Kim ◽  
Kug-Hwan Roh ◽  
Seong Wook Kim ◽  
Sung Jae Park ◽  
Na-Young Lim ◽  
...  
Keyword(s):  
Mouse Model ◽  
Choroidal Neovascularization ◽  
Type I

scholarly journals CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration

2020 ◽  
Vol 7 (5) ◽  
pp. 835-837 ◽  
Author(s):  
Changyang Zhou ◽  
Xinde Hu ◽  
Cheng Tang ◽  
Wenjia Liu ◽  
Shaoran Wang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Macular Degeneration ◽  
Choroidal Neovascularization ◽  
Age Related Macular Degeneration ◽  
Proof Of Concept ◽  
Rna Transcripts ◽  
Age Related ◽  
Rna Targeting

Summary RNA-targeting CRISPR system Cas13 offers an efficient approach for manipulating RNA transcripts in vitro. In this perspective, we provide a proof-of-concept demonstration that Cas13-mediated Vegfa knockdown in vivo could prevent the development of laser-induced CNV in mouse model of Age-related macular degeneration.

scholarly journals A Two-Stage Laser-Induced Mouse Model of Subretinal Fibrosis Secondary to Choroidal Neovascularization

2020 ◽  
Vol 9 (4) ◽  
pp. 3
Author(s):  
Karis Little ◽  
María Llorián-Salvador ◽  
Miao Tang ◽  
Xuan Du ◽  
Órlaith O'Shaughnessy ◽  
...  
Keyword(s):  
Mouse Model ◽  
Choroidal Neovascularization ◽  
Two Stage ◽  
Subretinal Fibrosis

scholarly journals The Sustained Delivery of Resveratrol or a Defined Grape Powder Inhibits New Blood Vessel Formation in a Mouse Model of Choroidal Neovascularization

Molecules ◽  
2014 ◽  
Vol 19 (11) ◽  
pp. 17578-17603 ◽  
Author(s):  
Mozhgan Kanavi ◽  
Soesiawati Darjatmoko ◽  
Shoujian Wang ◽  
Amir Azari ◽  
Mitra Farnoodian ◽  
...  
Keyword(s):  
Mouse Model ◽  
Blood Vessel ◽  
Choroidal Neovascularization ◽  
Sustained Delivery ◽  
Blood Vessel Formation ◽  
Vessel Formation

scholarly journals Triptolide attenuates laser-induced choroidal neovascularization via M2 macrophage in a mouse model

2020 ◽  
Vol 129 ◽  
pp. 110312 ◽  
Author(s):  
Kunbei Lai ◽  
Yajun Gong ◽  
Wenbo Zhao ◽  
Longhui Li ◽  
Chuangxin Huang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Choroidal Neovascularization ◽  
M2 Macrophage

scholarly journals Human Vascular Endothelial Growth Factor A165 Expression Induces the Mouse Model of Neovascular Age-Related Macular Degeneration

Genes ◽  
2018 ◽  
Vol 9 (9) ◽  
pp. 438 ◽  
Author(s):  
Emmi Kokki ◽  
Tommi Karttunen ◽  
Venla Olsson ◽  
Kati Kinnunen ◽  
Seppo Ylä-Herttuala
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Gene Transfer ◽  
Mouse Model ◽  
Choroidal Neovascularization ◽  
Age Related Macular Degeneration ◽  
Vascular Endothelial ◽  
Time Points ◽  
Age Related ◽  
Endothelial Growth Factor

Vascular endothelial growth factor (VEGF) expression induces age-related macular degeneration (AMD), which is a common vision-threatening disease due to choroidal neovascularization and a fibrovascular membrane. We describe a mouse model of neovascular AMD with the local expression of human VEGF-A165 in the eye. We use a transgenic mouse in which human VEGF-A165 has been silenced with the loxP-STOP fragment. The choroidal neovascularization and human VEGF-A165 expression in the mouse are induced by subretinal adenoviral Cre gene delivery. Cre gene transfer is compared with adenoviral LacZ gene transfer control. We characterize the AMD phenotype and changes in the vasculature by using fluorescein angiography, optical coherence tomography, and immunohistochemistry. At early time points, mice exhibit increases in retinal thickness (348 ± 114 µm vs. 231 ± 32 µm) and choroidal neovascularization area (12000 ± 15174 µm2 vs. 2169 ± 3495 µm2) compared with the control. At later time points, choroidal neovascularization develops into subretinal fibrovascular membrane. Human VEGF-A165 expression lasts several weeks. In conclusion, the retinas display vascular abnormalities consistent with choroidal neovascularization. Together with immunohistochemical findings, these changes resemble clinical AMD-like ocular pathologies. We conclude that this mouse model of Cre-induced choroidal neovascularization is useful for mimicking the pathogenesis of AMD, studying the effects of human VEGF-A165 in the retina, and evaluating anti-VEGF treatments for choroidal neovascularization.

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