An In Vivo Confocal Masked Study on Corneal Epithelium and Subbasal Nerves in Patients with Dry Eye

2004 ◽  
Vol 45 (9) ◽  
pp. 3030 ◽  
Author(s):  
Jose´ M. Beni´tez del Castillo ◽  
Mohamed A. S. Wasfy ◽  
Cristina Fernandez ◽  
Julian Garcia-Sanchez
Keyword(s):  
Dry Eye ◽  
Corneal Epithelium

scholarly journals Sleep Deprivation Induces Dry Eye Through Inhibition of PPARα Expression in Corneal Epithelium

2018 ◽  
Vol 59 (13) ◽  
pp. 5494 ◽  
Author(s):  
Liying Tang ◽  
Xue Wang ◽  
Jieli Wu ◽  
San Ming Li ◽  
Zhaoqiang Zhang ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Dry Eye ◽  
Corneal Epithelium

scholarly journals Kynurenic Acid Accelerates Healing of Corneal Epithelium In Vitro and In Vivo

2021 ◽  
Vol 14 (8) ◽  
pp. 753
Author(s):  
Anna Matysik-Woźniak ◽  
Waldemar A. Turski ◽  
Monika Turska ◽  
Roman Paduch ◽  
Mirosław Łańcut ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Corneal Epithelium ◽  
Kynurenic Acid ◽  
Pharmaceutical Products ◽  
Corneal Epithelial Cell ◽  
Eye Drops ◽  
Conjunctival Epithelium ◽  
Corneal Erosion

Kynurenic acid (KYNA) is an endogenous compound with a multidirectional effect. It possesses antiapoptotic, anti-inflammatory, and antioxidative properties that may be beneficial in the treatment of corneal injuries. Moreover, KYNA has been used successfully to improve the healing outcome of skin wounds. The aim of the present study is to evaluate the effects of KYNA on corneal and conjunctival cells in vitro and the re-epithelization of corneal erosion in rabbits in vivo. Normal human corneal epithelial cell (10.014 pRSV-T) and conjunctival epithelial cell (HC0597) lines were used. Cellular metabolism, cell viability, transwell migration, and the secretion of IL-1β, IL-6, and IL-10 were determined. In rabbits, after corneal de-epithelization, eye drops containing 0.002% and 1% KYNA were applied five times a day until full recovery. KYNA decreased metabolism but did not affect the proliferation of the corneal epithelium. It decreased both the metabolism and proliferation of conjunctival epithelium. KYNA enhanced the migration of corneal but not conjunctival epithelial cells. KYNA reduced the secretion of IL-1β and IL-6 from the corneal epithelium, leaving IL-10 secretion unaffected. The release of all studied cytokines from the conjunctival epithelium exposed to KYNA was unchanged. KYNA at higher concentration accelerated the healing of the corneal epithelium. These favorable properties of KYNA suggest that KYNA containing topical pharmaceutical products can be used in the treatment of ocular surface diseases.

scholarly journals The core planar cell polarity gene, Vangl2 , directs adult corneal epithelial cell alignment and migration

2016 ◽  
Vol 3 (10) ◽  
pp. 160658 ◽  
Author(s):  
Amy S. Findlay ◽  
D. Alessio Panzica ◽  
Petr Walczysko ◽  
Amy B. Holt ◽  
Deborah J. Henderson ◽  
...  
Keyword(s):  
Cell Migration ◽  
Epithelial Cells ◽  
Cell Polarity ◽  
Corneal Epithelium ◽  
Planar Cell Polarity ◽  
Corneal Epithelial Cell ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
The Core

This study shows that the core planar cell polarity (PCP) genes direct the aligned cell migration in the adult corneal epithelium, a stratified squamous epithelium on the outer surface of the vertebrate eye. Expression of multiple core PCP genes was demonstrated in the adult corneal epithelium. PCP components were manipulated genetically and pharmacologically in human and mouse corneal epithelial cells in vivo and in vitro . Knockdown of VANGL2 reduced the directional component of migration of human corneal epithelial (HCE) cells without affecting speed. It was shown that signalling through PCP mediators, dishevelled, dishevelled-associated activator of morphogenesis and Rho-associated protein kinase directs the alignment of HCE cells by affecting cytoskeletal reorganization. Cells in which VANGL2 was disrupted tended to misalign on grooved surfaces and migrate across, rather than parallel to the grooves. Adult corneal epithelial cells in which Vangl2 had been conditionally deleted showed a reduced rate of wound-healing migration. Conditional deletion of Vangl2 in the mouse corneal epithelium ablated the normal highly stereotyped patterns of centripetal cell migration in vivo from the periphery (limbus) to the centre of the cornea. Corneal opacity owing to chronic wounding is a major cause of degenerative blindness across the world, and this study shows that Vangl2 activity is required for directional corneal epithelial migration.

scholarly journals The immunoregulatory role of corneal epithelium-derived thrombospondin-1 in dry eye disease

2018 ◽  
Vol 16 (4) ◽  
pp. 470-477 ◽  
Author(s):  
Xuhua Tan ◽  
Yihe Chen ◽  
William Foulsham ◽  
Afsaneh Amouzegar ◽  
Takenori Inomata ◽  
...  
Keyword(s):  
Dry Eye ◽  
Corneal Epithelium ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
Thrombospondin 1

scholarly journals Effective In Vivo Topical Delivery of siRNA and Gene Silencing in Intact Corneal Epithelium Using a Modified Cell-Penetrating Peptide

2019 ◽  
Vol 17 ◽  
pp. 891-906 ◽  
Author(s):  
Davide Schiroli ◽  
María J. Gómara ◽  
Eleonora Maurizi ◽  
Sarah D. Atkinson ◽  
Laura Mairs ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Corneal Epithelium ◽  
Topical Delivery ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

scholarly journals Corneal Epithelial Immune Dendritic Cell Alterations in Subtypes of Dry Eye Disease: A Pilot In Vivo Confocal Microscopic Study

2015 ◽  
Vol 56 (12) ◽  
pp. 7179 ◽  
Author(s):  
Ahmad Kheirkhah ◽  
Raheleh Rahimi Darabad ◽  
Andrea Cruzat ◽  
Amir Reza Hajrasouliha ◽  
Deborah Witkin ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Microscopic Study ◽  
Dry Eye ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
Corneal Epithelial ◽  
Cell Alterations ◽  
Confocal Microscopic Study
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