Expression of the α4 Integrin Subunit Gene Promoter Is Modulated by the Transcription Factor Pax-6 in Corneal Epithelial Cells

2004 ◽  
Vol 45 (6) ◽  
pp. 1692 ◽  
Author(s):  
Karine Zaniolo ◽  
Steeve Leclerc ◽  
Ales Cvekl ◽  
Luc Vallie`res ◽  
Richard Bazin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Epithelial Cells ◽  
Gene Promoter ◽  
Integrin Subunit ◽  
Subunit Gene ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial

scholarly journals Ets family transcription factor ESE-1 is expressed in corneal epithelial cells and is involved in their differentiation

2000 ◽  
Vol 97 (1-2) ◽  
pp. 27-34 ◽  
Author(s):  
Naoko Yoshida ◽  
Shosei Yoshida ◽  
Makoto Araie ◽  
Hiroshi Handa ◽  
Yo-ichi Nabeshima
Keyword(s):  
Transcription Factor ◽  
Epithelial Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Ets Family

scholarly journals Regulation of K3 keratin gene transcription by Sp1 and AP-2 in differentiating rabbit corneal epithelial cells.

1997 ◽  
Vol 17 (6) ◽  
pp. 3056-3064 ◽  
Author(s):  
T T Chen ◽  
R L Wu ◽  
F Castro-Munozledo ◽  
T T Sun
Keyword(s):  
Epithelial Cells ◽  
Gene Promoter ◽  
Epithelial Differentiation ◽  
Binding Motif ◽  
Basal Cells ◽  
High Concentration ◽  
Corneal Epithelial Cells ◽  
Keratin Gene ◽  
Corneal Epithelial ◽  
Basal Keratinocytes

Rabbit corneal epithelial cells cultured in the presence of 3T3 feeder cells undergo biochemical differentiation, as evidenced by their initial expression of K5 and K14 keratins characteristic of basal keratinocytes, followed by the subsequent expression of K3 and K12 keratin markers of corneal epithelial differentiation. Previous data established that mutations of an Sp1 site in a DNA element, E, that contains overlapping Sp1 and AP-2 motifs reduce K3 gene promoter activity by 70% in transfection assays. We show here that Sp1 activates while AP-2 represses the K3 promoter. Although undifferentiated corneal epithelial basal cells express equal amounts of Sp1 and AP-2 DNA-binding activities, the differentiated cells down-regulate their Sp1 activity slightly but their AP-2 activity drastically, thus resulting in a six- to sevenfold increase in the Sp1/AP-2 ratio. This change coincides with the activation and suppression of the differentiation-related K3 gene and the basal cell-related K14 keratin gene, respectively. In addition, we show that polyamines, which are present in a high concentration in proliferating basal keratinocytes, can inhibit the binding of Sp1 to its cognate binding motif but not that of AP-2. These results suggest that the relatively low Sp1/AP-2 ratio as well as the polyamine-mediated inhibition of Sp1 binding to the E motif may account, in part, for the suppression of the K3 gene in corneal epithelial basal cells, while the elevated Sp1/AP-2 ratio may be involved in activating the K3 gene in differentiated corneal epithelial cells. Coupled with the previous demonstration that AP-2 activates the K14 gene in basal cells, the switch of the Sp1/AP-2 ratio during corneal epithelial differentiation may play a role in the reciprocal expression of the K3 and K14 genes in the basal and suprabasal cell layers.

Functional Impact of Collagens on the Activity Directed by the Promoter of theα5Integrin Subunit Gene in Corneal Epithelial Cells

2015 ◽  
Vol 56 (11) ◽  
pp. 6217 ◽  
Author(s):  
Jennifer Lake ◽  
Karine Zaniolo ◽  
Marie-Ève Gingras ◽  
Camille Couture ◽  
Christian Salesse ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Subunit Gene ◽  
Corneal Epithelial Cells ◽  
Functional Impact ◽  
Corneal Epithelial

Carboxymethyl β-glucan Binds to Corneal Epithelial Cells and Increases Cell Adhesion to Laminin and Resistance to Oxidative Stress

Cornea ◽  
2007 ◽  
Vol 26 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Marco Porcu ◽  
Francesco Guarna ◽  
Laura Formentini ◽  
Giuseppe Faraco ◽  
Silvia Fossati ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Adhesion ◽  
Epithelial Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial

In Vitro Toxicity of Netilmicin and Ofloxacin on Corneal Epithelial Cells

Cornea ◽  
2003 ◽  
Vol 22 (5) ◽  
pp. 468-472 ◽  
Author(s):  
Anna Claudia Scuderi ◽  
Grazia Maria Paladino ◽  
Clara Marino ◽  
Francesco Trombetta
Keyword(s):  
Epithelial Cells ◽  
In Vitro Toxicity ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial

Recombinant TIMP-1 and -2 enhance the proliferation of rabbit corneal epithelial cells in vitro and the spreading of rabbit corneal epithelium in situ

1998 ◽  
Vol 17 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Shizuya Saika ◽  
Yoshiji Kawashima ◽  
Yuka Okada ◽  
Sai-Ichi Tanaka ◽  
Osamu Yamanaka ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Corneal Epithelium ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial
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