scholarly journals A free and open-source toolkit of three-dimensional models and software to study face perception

2019 ◽  
Vol 19 (10) ◽  
pp. 227a
Author(s):  
Jason S Hays ◽  
Claudia Wong ◽  
Fabian Soto
2019 ◽  
Author(s):  
Jason Scott Hays ◽  
Claudia Wong ◽  
Fabian Soto

A problem in the study of face perception is that results can be confounded by poor stimulus control. Ideally, experiments should precisely manipulate facial features under study and tightly control irrelevant features. Software for 3D face modeling provides such control, but there is a lack of free and open source alternatives specifically created for face perception research. Here, we provide such tools by expanding the open-source software MakeHuman. We present a database of 27 identity models and 6 expression pose models (sadness, anger, happiness, disgust, fear, and surprise), together with software to manipulate the models in ways that are common in the face perception literature, allowing researchers to: (1) create a sequence of renders from interpolations between two or more 3D models (differing in identity, expression, and/or pose), resulting in a “morphing” sequence; (2) create renders by extrapolation in a direction of face space, obtaining 3D “anti-faces” and caricatures; (3) obtain videos of dynamic faces from rendered images; (4) obtain average face models; (5) standardize a set of models so that they differ only in selected facial shape features, and (6) communicate with experiment software (e.g., PsychoPy) to render faces dynamically online. These tools vastly improve both the speed at which face stimuli can be produced and the level of control that researchers have over face stimuli. We validate the face model database and software tools through a small study on human perceptual judgments of stimuli produced with the toolkit.


1975 ◽  
Vol 39 (8) ◽  
pp. 544-546
Author(s):  
HL Wakkerman ◽  
GS The ◽  
AJ Spanauf

2020 ◽  
Vol 17 (4) ◽  
pp. 342-351
Author(s):  
Sergio A. Durán-Pérez ◽  
José G. Rendón-Maldonado ◽  
Lucio de Jesús Hernandez-Diaz ◽  
Annete I. Apodaca-Medina ◽  
Maribel Jiménez-Edeza ◽  
...  

Background: The protozoan Giardia duodenalis, which causes giardiasis, is an intestinal parasite that commonly affects humans, mainly pre-school children. Although there are asymptomatic cases, the main clinical features are chronic and acute diarrhea, nausea, abdominal pain, and malabsorption syndrome. Little is currently known about the virulence of the parasite, but some cases of chronic gastrointestinal alterations post-infection have been reported even when the infection was asymptomatic, suggesting that the cathepsin L proteases of the parasite may be involved in the damage at the level of the gastrointestinal mucosa. Objective: The aim of this study was the in silico identification and characterization of extracellular cathepsin L proteases in the proteome of G. duodenalis. Methods: The NP_001903 sequence of cathepsin L protease from Homo sapienswas searched against the Giardia duodenalisproteome. The subcellular localization of Giardia duodenaliscathepsin L proteases was performed in the DeepLoc-1.0 server. The construction of a phylogenetic tree of the extracellular proteins was carried out using the Molecular Evolutionary Genetics Analysis software (MEGA X). The Robetta server was used for the construction of the three-dimensional models. The search for possible inhibitors of the extracellular cathepsin L proteases of Giardia duodenaliswas performed by entering the three-dimensional structures in the FINDSITEcomb drug discovery tool. Results: Based on the amino acid sequence of cathepsin L from Homo sapiens, 8 protein sequences were identified that have in their modular structure the Pept_C1A domain characteristic of cathepsins and two of these proteins (XP_001704423 and XP_001704424) are located extracellularly. Threedimensional models were designed for both extracellular proteins and several inhibitory ligands with a score greater than 0.9 were identified. In vitrostudies are required to corroborate if these two extracellular proteins play a role in the virulence of Giardia duodenalisand to discover ligands that may be useful as therapeutic targets that interfere in the mechanism of pathogenesis generated by the parasite. Conclusion: In silicoanalysis identified two proteins in the Giardia duodenalisprotein repertoire whose characteristics allowed them to be classified as cathepsin L proteases, which may be secreted into the extracellular medium to act as virulence factors. Three-dimensional models of both proteins allowed the identification of inhibitory ligands with a high score. The results suggest that administration of those compounds might be used to block the endopeptidase activity of the extracellular cathepsin L proteases, interfering with the mechanisms of pathogenesis of the protozoan parasite Giardia duodenalis.


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