scholarly journals High speed adaptive optics parallel confocal ophthalmoscopy

2017 ◽  
Vol 17 (7) ◽  
pp. 35
Author(s):  
Yuhua Zhang ◽  
Jing Lu ◽  
Boyu Gu ◽  
Xiaolin Wang
Keyword(s):  
Adaptive Optics ◽  
High Speed

scholarly journals A micromirror array with annular partitioning for high-speed random-access axial focusing

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Nathan Tessema Ersumo ◽  
Cem Yalcin ◽  
Nick Antipa ◽  
Nicolas Pégard ◽  
Laura Waller ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Visual Information ◽  
High Speed ◽  
Random Access ◽  
General Purpose ◽  
Phase Shifting ◽  
Optical Systems ◽  
Piston Motion ◽  
Refresh Rate ◽  
Circular Symmetry

Abstract Dynamic axial focusing functionality has recently experienced widespread incorporation in microscopy, augmented/virtual reality (AR/VR), adaptive optics and material processing. However, the limitations of existing varifocal tools continue to beset the performance capabilities and operating overhead of the optical systems that mobilize such functionality. The varifocal tools that are the least burdensome to operate (e.g. liquid crystal, elastomeric or optofluidic lenses) suffer from low (≈100 Hz) refresh rates. Conversely, the fastest devices sacrifice either critical capabilities such as their dwelling capacity (e.g. acoustic gradient lenses or monolithic micromechanical mirrors) or low operating overhead (e.g. deformable mirrors). Here, we present a general-purpose random-access axial focusing device that bridges these previously conflicting features of high speed, dwelling capacity and lightweight drive by employing low-rigidity micromirrors that exploit the robustness of defocusing phase profiles. Geometrically, the device consists of an 8.2 mm diameter array of piston-motion and 48-μm-pitch micromirror pixels that provide 2π phase shifting for wavelengths shorter than 1100 nm with 10–90% settling in 64.8 μs (i.e., 15.44 kHz refresh rate). The pixels are electrically partitioned into 32 rings for a driving scheme that enables phase-wrapped operation with circular symmetry and requires <30 V per channel. Optical experiments demonstrated the array’s wide focusing range with a measured ability to target 29 distinct resolvable depth planes. Overall, the features of the proposed array offer the potential for compact, straightforward methods of tackling bottlenecked applications, including high-throughput single-cell targeting in neurobiology and the delivery of dense 3D visual information in AR/VR.

scholarly journals Dual-view light-sheet imaging through tilted glass interface using a deformable mirror

2020 ◽  
Author(s):  
N Vladimirov ◽  
F Preusser ◽  
J Wisniewski ◽  
Z Yaniv ◽  
RA Desai ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Microfluidic Channel ◽  
Microfluidic Devices ◽  
Light Sheet ◽  
Stochastic Gradient Descent ◽  
Deformable Mirror ◽  
Optical Aberrations ◽  
Light Sheet Microscopy ◽  
Gradient Descent Algorithm

AbstractLight-sheet microscopy has become one of the primary tools for imaging live developing organisms because of its high speed, low phototoxicity, and optical sectioning capabilities. Detection from multiple sides (multi-view imaging) additionally allows nearly isotropic resolution via computational merging of the views. However, conventional light-sheet microscopes require that the sample is suspended in a gel to allow optical access from two or more sides. At the same time, the use of microfluidic devices is highly desirable for many experiments, but geometric constrains and strong optical aberrations caused by the coverslip titled relative to objectives make the use of multi-view lightsheet challenging for microfluidics.In this paper we describe the use of adaptive optics (AO) to enable multi-view light-sheet microscopy in such microfluidic setup by correcting optical aberrations introduced by the tilted coverslip. The optimal shape of deformable mirror is computed by an iterative stochastic gradient-descent algorithm that optimizes PSF in two orthogonal planes simultaneously. Simultaneous AO correction in two optical arms is achieved via a knife-edge mirror that splits excitation path and combines the detection path.We characterize the performance of this novel microscope setup and, by dual-view light-sheet imaging of C.elegans inside a microfluidic channel, demonstrate a drastic improvement of image quality due to AO and dual-view reconstruction. Our microscope design allows multi-view light-sheet microscopy with microfluidic devices for precisely controlled experimental conditions and high-content screening.

scholarly journals High-speed adaptive optics line-scan OCT for cellular-resolution optoretinography

2020 ◽  
Vol 11 (9) ◽  
pp. 5274 ◽  
Author(s):  
Vimal Prabhu Pandiyan ◽  
Xiaoyun Jiang ◽  
Aiden Maloney-Bertelli ◽  
James A. Kuchenbecker ◽  
Utkarsh Sharma ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Line Scan ◽  
Cellular Resolution

scholarly journals Observing the Cell in Its Native State: Imaging Subcellular Dynamics in Multicellular Organisms

2018 ◽  
Author(s):  
Tsung-Li Liu ◽  
Srigokul Upadhyayula ◽  
Daniel E. Milkie ◽  
Ved Singh ◽  
Kai Wang ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Developmental Stages ◽  
Phenotypic Diversity ◽  
Metastatic Cancer ◽  
Light Sheet ◽  
Intrinsic Variability ◽  
Spatiotemporal Resolution ◽  
Light Sheet Microscopy

AbstractTrue physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution without inducing undue stress on either. We combined lattice light sheet microscopy with two-channel adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages, and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.One Sentence SummaryCombining lattice light sheet microscopy with adaptive optics enables high speed, high resolution in vivo 3D imaging of dynamic processes inside cells under physiological conditions within their parent organisms.

HIGH SPEED SIMULTANEOUS SLO/OCT IMAGING OF THE HUMAN RETINA WITH ADAPTIVE OPTICS – Oral Paper

2008 ◽  
Author(s):  
MICHAEL PIRCHER ◽  
ROBERT J. ZAWADZKI ◽  
JULIA EVANS ◽  
JOHN S. WERNER ◽  
CHRISTOPH K. HITZENBERGER
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Human Retina ◽  
Oct Imaging

High Speed pn-CCDs as Wave Front Sensors in Adaptive Optics Systems

2000 ◽  
pp. 217-225
Author(s):  
R. Hartmann ◽  
N. Krause ◽  
N. Meidinger ◽  
L. Strüder ◽  
J. Trümper ◽  
...  
Keyword(s):  
Wave Front ◽  
Adaptive Optics ◽  
High Speed
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