Label-Free Electrochemical DNA Sensor for Enteroviruses Detection

2016 ◽  
Vol 14 (11) ◽  
pp. 1112-1116
Author(s):  
Ichrak Slama ◽  
Nabil Ben Salem Abid ◽  
Ines Othman ◽  
Ibtissem Ben Assaker ◽  
Radhouane Chtourou ◽  
...  
2014 ◽  
Vol 38 (12) ◽  
pp. 5918-5924 ◽  
Author(s):  
Linxia Fang ◽  
Kejing Huang ◽  
Baoling Zhang ◽  
Yujie Liu ◽  
Qiuyu Zhang

A highly sensitive electrochemical DNA sensor was constructed by homogenously distributing Au nanoparticles (AuNPs) on a flower-like 3D ZnO superstructure–chitosan (CS) matrix.


2015 ◽  
Vol 7 (7) ◽  
pp. 3164-3168 ◽  
Author(s):  
Na Zhang ◽  
Keying Zhang ◽  
Li Zhang ◽  
Hongyan Wang ◽  
Hongwei Shi ◽  
...  

Schematic representation of the immobilization of p-ssDNA and hybridization detection.


2017 ◽  
Vol 53 (46) ◽  
pp. 6215-6218 ◽  
Author(s):  
Xinping Liu ◽  
Zhengqing Yan ◽  
Yuhuan Sun ◽  
Jinsong Ren ◽  
Xiaogang Qu

A label-free ratiometric electrochemical determination of GSH by DNA metallization-mediated HCR amplification is reported.


2021 ◽  
Author(s):  
Tuan Chu ◽  
Luyen Thi Tran ◽  
Hoang Vinh Tran ◽  
Trung Tran ◽  
Nghia Trong Nguyen ◽  
...  

Abstract We describe in this report a development of label-free electrochemical DNA sensor based on a novel nanostructured electrode of multi-walled carbon nanotubes (MWCNTs)/ nano-flowers-like manganese dioxide (MnO2)/polyaniline nanowires (PANi NWs) nanocomposite. The nanocomposite was synthesized in-situ onto an interdigitated platinum microelectrode (Pt) using a combination of chemical and electrochemical synthesis methods: chemical preparation of MWCNTs/MnO2 and electropolymerization of PANi NWs. The fabricated MWCNTs/MnO2/PANi NWs was then used to develop a label-free electrochemical DNA sensor for a specific gene of Escherichia coli (E.coli) O157:H7 detection. The MWCNTs/MnO2/PANi NWs modified Pt electrode’s surface can facilitate for probe DNA strands immobilization and, therefore the electrochemical signal of the DNA sensors has been improved. The electrochemical impedance spectroscopy (EIS) measurements were conducted to investigate the output signals generated by the specific binding of probe and target DNA sequences. Obtained results indicated that the developed electrochemical biosensor can detect the target DNA in the linear range of 5 pM to 500 nM with a low limit of detection (LOD) at 4.42 × 10 –13 M. The research results demonstrated that the MWCNTs/MnO2/PANi NWs nanocomposite-based electrochemical DNA sensor has a great potential application to the development of highly sensitive and selective electrochemical DNA sensors to detect pathogenic agents.


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