A label-free ratiometric electrochemical DNA sensor for monitoring intracellular redox homeostasis

2017 ◽  
Vol 53 (46) ◽  
pp. 6215-6218 ◽  
Author(s):  
Xinping Liu ◽  
Zhengqing Yan ◽  
Yuhuan Sun ◽  
Jinsong Ren ◽  
Xiaogang Qu
Keyword(s):  
Redox Homeostasis ◽  
Electrochemical Determination ◽  
Dna Sensor ◽  
Label Free ◽  
Electrochemical Dna Sensor ◽  
Intracellular Redox

A label-free ratiometric electrochemical determination of GSH by DNA metallization-mediated HCR amplification is reported.

ELECTROCHEMICAL DNA SENSOR TECHNOLOGY FOR MONITORING OF DRUG–DNA INTERACTIONS

NANO ◽  
2008 ◽  
Vol 03 (04) ◽  
pp. 229-232 ◽  
Author(s):  
A. ERDEM ◽  
H. KARADENIZ ◽  
A. CALISKAN ◽  
A. VASEASHTA
Keyword(s):  
Drug Delivery ◽  
Small Molecules ◽  
Anticancer Drugs ◽  
Point Of Care ◽  
Sensor Technology ◽  
Electrochemical Biosensors ◽  
Dna Sensor ◽  
Dna Interactions ◽  
Electrochemical Dna Sensor

The objective of this investigation is to understand the nature and dynamics of binding small molecules to bio-macromolecules using electrochemical methods. The investigation pertaining to the design of site- and conformation-specific reagents provides a rationale for new studies of drug delivery design. Some anticancer drugs and DNA interactions have been undertaken by using a variety of techniques. Determination of interaction between DNA and DNA-targeted molecules would be valuable in the design of molecule-specific electrochemical biosensors for applications in diagnostics, development of drugs for chemotherapy, and as a biotechnological tool for DNA-based point-of-care diagnosis.

scholarly journals A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

2020 ◽  
Vol 187 (5) ◽  
Author(s):  
Jagriti Sethi ◽  
Michiel Van Bulck ◽  
Ahmed Suhail ◽  
Mina Safarzadeh ◽  
Ana Perez-Castillo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Label Free ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

AbstractA label-free biosensor is developed for the determination of plasma-based Aβ1–42 biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) is achieved to facilitate immobilization of H31L21 antibody. The effect of these modifications were studied with morphological, spectral and electrochemical techniques. The response of the biosensor was evaluated using differential pulse voltammetry (DPV). The data was acquired at a working potential of ~ 180 mV and a scan rate of 50 mV s−1. A low limit of detection (LOD) of 2.398 pM is achieved over a wide linear range from 11 pM to 55 nM. The biosensor exhibits excellent specificity over Aβ1–40 and ApoE ε4 interfering species. Thus, it provides a viable tool for electrochemical determination of Aβ1–42. Spiked human and mice plasmas were used for the successful validation of the sensing platform in bio-fluidic samples. The results obtained from mice plasma analysis concurred with the immunohistochemistry (IHC) and magnetic resonance imaging (MRI) data obtained from brain analysis.

Label-Free Electrochemical DNA Sensor for Enteroviruses Detection

2016 ◽  
Vol 14 (11) ◽  
pp. 1112-1116
Author(s):  
Ichrak Slama ◽  
Nabil Ben Salem Abid ◽  
Ines Othman ◽  
Ibtissem Ben Assaker ◽  
Radhouane Chtourou ◽  
...  
Keyword(s):  
Dna Sensor ◽  
Label Free ◽  
Electrochemical Dna Sensor

scholarly journals Development of Electrochemical DNA Sensor for The Determination of Anaplasma Phagocytophilum

2017 ◽  
Vol 38 (4) ◽  
pp. 748-758
Author(s):  
Adil ELİK ◽  
Gültekin GÖKÇE ◽  
Çağdaş CEYLAN
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Dna Sensor ◽  
Electrochemical Dna Sensor

A label-free electrochemistry biosensor based flower-like 3-dimensional ZnO superstructures for detection of DNA arrays

2014 ◽  
Vol 38 (12) ◽  
pp. 5918-5924 ◽  
Author(s):  
Linxia Fang ◽  
Kejing Huang ◽  
Baoling Zhang ◽  
Yujie Liu ◽  
Qiuyu Zhang
Keyword(s):  
Au Nanoparticles ◽  
Dna Sensor ◽  
Label Free ◽  
Dna Arrays ◽  
3 Dimensional ◽  
Electrochemical Dna Sensor ◽  
Highly Sensitive

A highly sensitive electrochemical DNA sensor was constructed by homogenously distributing Au nanoparticles (AuNPs) on a flower-like 3D ZnO superstructure–chitosan (CS) matrix.

Electrochemical determination of biophenol oleuropein using a simple label-free DNA biosensor

2015 ◽  
Vol 101 ◽  
pp. 52-57 ◽  
Author(s):  
Maryam Mohamadi ◽  
Ali Mostafavi ◽  
Masoud Torkzadeh-Mahani
Keyword(s):  
Electrochemical Determination ◽  
Dna Biosensor ◽  
Label Free ◽  
Free Dna

scholarly journals Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-Aptamer

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Hongliang Guan ◽  
Zhike He
Keyword(s):  
Conjugated Polymer ◽  
Specific Binding ◽  
Water Soluble ◽  
Dna Sensor ◽  
Label Free ◽  
Binding Reaction ◽  
Molecular Sensors ◽  
Free Dna ◽  
Highly Sensitive

Water-soluble fluorescent conjugated polymer is a promising material which could be used as an optical platform in highly sensitive molecular sensors. In this paper, a simple label-free DNA sensor, which consisted of a poly(3-alkoxy-4-methylthiophene) and an aptamer, was used to detect L-argininamide (L-Arm). Due to the specific binding reaction between L-Arm and its aptamer, the proposed method can easily determinate the L-Arm through the recovery of fluorescence without any modification. Other ions or similar molecules had little effect on the detection. Moreover, there was a linear relationship between fluorescence intensity and the concentration of L-Arm. The detection limit of L-Arm was as low as 4.7 nM.

Sign in / Sign up

Export Citation Format

Share Document