Peptide Immobilization on GaAs Surfaces and the Application to Label-Free Detection of Antigen-Antibody Interactions Using Multiple Internal Reflection Infrared Spectroscopy

2008 ◽  
Vol 6 (4) ◽  
pp. 613-617 ◽  
Author(s):  
Ayumi Hirano ◽  
Kota Onodera ◽  
Ko-ichiro Miyamoto ◽  
Yasuo Kimura ◽  
Masatoshi Kataoka ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Internal Reflection ◽  
Label Free ◽  
Label Free Detection ◽  
Antigen Antibody ◽  
Antibody Interactions

scholarly journals Affinity Sensors for the Diagnosis of COVID-19

Micromachines ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 390
Author(s):  
Maryia Drobysh ◽  
Almira Ramanaviciene ◽  
Roman Viter ◽  
Arunas Ramanavicius
Keyword(s):  
Field Effect Transistors ◽  
Resonance Field ◽  
Analytical Signal ◽  
Nucleic Acid Hybridization ◽  
Detection Methods ◽  
Label Free ◽  
Infected People ◽  
Label Free Detection ◽  
Main Instrument ◽  
Antigen Antibody

The coronavirus disease 2019 (COVID-19) outbreak caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was proclaimed a global pandemic in March 2020. Reducing the dissemination rate, in particular by tracking the infected people and their contacts, is the main instrument against infection spreading. Therefore, the creation and implementation of fast, reliable and responsive methods suitable for the diagnosis of COVID-19 are required. These needs can be fulfilled using affinity sensors, which differ in applied detection methods and markers that are generating analytical signals. Recently, nucleic acid hybridization, antigen-antibody interaction, and change of reactive oxygen species (ROS) level are mostly used for the generation of analytical signals, which can be accurately measured by electrochemical, optical, surface plasmon resonance, field-effect transistors, and some other methods and transducers. Electrochemical biosensors are the most consistent with the general trend towards, acceleration, and simplification of the bioanalytical process. These biosensors mostly are based on the determination of antigen-antibody interaction and are robust, sensitive, accurate, and sometimes enable label-free detection of an analyte. Along with the specification of biosensors, we also provide a brief overview of generally used testing techniques, and the description of the structure, life cycle and immune host response to SARS-CoV-2, and some deeper details of analytical signal detection principles.

In VitroDiagnostic Device with a Bio-Coupled Gate Field Effect Transistor

2018 ◽  
Vol 12 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Toshiya Sakata ◽  
Keyword(s):  
Semiconductor Device ◽  
Direct Detection ◽  
Cell Communication ◽  
Label Free ◽  
Cellular Functions ◽  
Biological Phenomena ◽  
Communication Processes ◽  
Antigen Antibody ◽  
Antibody Interactions

In this study, we report a simple and rapid biosensing method for the analysis andin vitromonitoring of biological processes, including DNAbinding events, antigen-antibody interactions, and cellular functions, using a semiconductor device. Most biological phenomena involve cell-cell communication processes that are mediated by the transport of sodium or potassium ions and other charged biomolecules, such as DNA, across ion channels in the cell membrane. Therefore, our approach focused on the direct detection of changes in ion concentrations by utilizing a semiconductor-based biosensor device. Our results demonstrated that our semiconductor-based biosensor platform achieves label-free and noninvasive biosensing that is suitable forin vitrodiagnosis.

scholarly journals A Differential Detection Method Based on a Linear Weak Measurement System

Sensors ◽  
2019 ◽  
Vol 19 (11) ◽  
pp. 2473
Author(s):  
Nian Xiong ◽  
Tian Guan ◽  
Yang Xu ◽  
Lixuan Shi ◽  
Suyi Zhong ◽  
...  
Keyword(s):  
Measurement System ◽  
Total Internal Reflection ◽  
High Stability ◽  
Detection Method ◽  
Weak Measurement ◽  
Internal Reflection ◽  
Differential Detection ◽  
Label Free ◽  
Label Free Detection ◽  
Linear Differential

Self-reference detection is necessary and important to a biosensor. The linear weak measurement system based on total internal reflection has attracted widespread attention due to its high stability, label-free detection, and easy integration. In this paper, we propose a differential detection method based on the linear total internal reflection weak measurement system. We introduce the half-wave plate (HWP) to convert the H light and the V light to each other, thereby obtaining the difference in phase change of the optical path before and after the HWP. Experiments show that the system can not only achieve differential detection, but also has high stability. The linear differential weak measurement system proposed in this paper not only provides a new differential measurement method for real-time biosensors, but also enriches the types of weak measurement sensors.

scholarly journals Sensitivity Improvement of an Impedimetric Immunosensor Using Functionalized Iron Oxide Nanoparticles

2009 ◽  
Vol 2009 ◽  
pp. 1-12 ◽  
Author(s):  
Imen Hafaid ◽  
Asma Gallouz ◽  
Walid Mohamed Hassen ◽  
Adnane Abdelghani ◽  
Zina Sassi ◽  
...  
Keyword(s):  
Iron Nanoparticles ◽  
Gold Surface ◽  
Antibody Immobilization ◽  
Label Free ◽  
Antibody Recognition ◽  
Label Free Detection ◽  
Measured Angle ◽  
Sensitivity Improvement ◽  
Antigen Antibody ◽  
Impedimetric Immunosensor

This work has explored the development of impedimetric immunosensors based on magnetic iron nanoparticles (IrNP) functionalized with streptavidin to which a biotinylated FAB part of the antibody has been bound using a biotin-streptavidin interaction. SPR analysis shows a deviation on the measured (angle) during antigen-antibody recognition whereas label free detection using by EIS allows us to monitor variation of polarization resistance. Before detection, layers were analyzed by FTIR and AFM. Compared to immobilization of antibody on bare gold surface using aminodecanethiol SAM, antibody immobilization on nanoparticles permitted to reach lower detection limit: 500 pg/ml instead of 1 ng/ml to in the case of EIS and 300 ng/ml instead of 4.5 μg/ml in the case of SPR. Thus, it permitted to improve the sensitivity: from 257.3 Ω⋅cm2⋅μg−1⋅mlto 1871 Ω⋅cm2⋅μg−1⋅mlin the case of EIS and from0.003°μg−1⋅mlto0.094°μg−1⋅mlin the case of SPR.

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