Label-Free Detection of Protein−Protein Interactions at the GaAs/Water Interface through Surface Infrared Spectroscopy:  Discrimination between Specific and Nonspecific Interactions by Using Secondary Structure Analysis

Langmuir ◽  
2007 ◽  
Vol 23 (24) ◽  
pp. 12287-12292 ◽  
Author(s):  
Kota Onodera ◽  
Ayumi Hirano-Iwata ◽  
Ko-ichiro Miyamoto ◽  
Yasuo Kimura ◽  
Masatoshi Kataoka ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Secondary Structure ◽  
Structure Analysis ◽  
Protein Interactions ◽  
Water Interface ◽  
Label Free ◽  
Protein Protein Interactions ◽  
Secondary Structure Analysis ◽  
Label Free Detection ◽  
Nonspecific Interactions

scholarly journals Label-Free Detection of Single Protein Molecules and Protein−Protein Interactions Using Synthetic Nanopores

2008 ◽  
Vol 80 (12) ◽  
pp. 4651-4658 ◽  
Author(s):  
Anpan Han ◽  
Marc Creus ◽  
Gregor Schürmann ◽  
Vincent Linder ◽  
Thomas R. Ward ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Label Free ◽  
Protein Protein Interactions ◽  
Label Free Detection ◽  
Protein Molecules ◽  
Single Protein

Automated, High-Throughput Infrared Spectroscopy for Secondary Structure Analysis of Protein Biopharmaceuticals

2020 ◽  
Vol 109 (10) ◽  
pp. 3223-3230
Author(s):  
Lucy L. Liu ◽  
Libo Wang ◽  
Jeffrey Zonderman ◽  
Jason C. Rouse ◽  
Hai-Young Kim
Keyword(s):  
Infrared Spectroscopy ◽  
Secondary Structure ◽  
Structure Analysis ◽  
High Throughput ◽  
Secondary Structure Analysis

scholarly journals Vertical Scanning Interferometry for Label-Free Detection of Peptide-Antibody Interactions

2019 ◽  
Vol 8 (2) ◽  
pp. 7
Author(s):  
Andrea Palermo ◽  
Richard Thelen ◽  
Laura Weber ◽  
Tobias Foertsch ◽  
Simone Rentschler ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Antibody Concentration ◽  
Label Free ◽  
Protein Protein Interactions ◽  
Peptide Arrays ◽  
Serum Dilution ◽  
Aspect Ratios ◽  
Peptide Antibody ◽  
Label Free Detection ◽  
Vertical Scanning Interferometry

Peptide microarrays are a fast-developing field enabling the mapping of linear epitopes in the immune response to vaccinations or diseases and high throughput studying of protein-protein interactions. In this respect, a rapid label-free measurement of protein layer topographies in the array format is of great interest but is also a great challenge due to the extremely low aspect ratios of the peptide spots. We have demonstrated the potential of vertical scanning interferometry (VSI) for a detailed morphological analysis of peptide arrays and binding antibodies. The VSI technique is shown to scan an array area of 5.1 square millimeters within 3–4 min at a resolution of 1.4 μm lateral and 0.1 nm vertical in the full automation mode. Topographies obtained by VSI do match the one obtained by AFM measurements, demonstrating the accuracy of the technique. A detailed topology of peptide-antibody layers on single spots was measured. Two different measurement regions are distinguished according to the antibody concentration. In the case of weakly diluted serum, the thickness of the antibody layer is independent of the serum dilution and corresponds to the physical thickness of the accumulated antibody layer. In strongly diluted serum, the thickness measured via VSI is linearly proportional to the serum dilution.

Label-Free Detection of Protein-Protein Interactions on Biochips

2005 ◽  
Vol 44 (34) ◽  
pp. 5480-5483 ◽  
Author(s):  
Woon-Seok Yeo ◽  
Dal-Hee Min ◽  
Robert W. Hsieh ◽  
Geoffrey L. Greene ◽  
Milan Mrksich
Keyword(s):  
Protein Interactions ◽  
Label Free ◽  
Protein Protein Interactions ◽  
Label Free Detection
Sign in / Sign up

Export Citation Format

Share Document