A Novel Method of Synthesis of Fluorescent Carbon Dots for Supersensitive and Selective Detection of Cancer Markers

2018 ◽  
Vol 18 (12) ◽  
pp. 8085-8093
Author(s):  
Jianrong Qi ◽  
Meixin Hu ◽  
Hongxia Li ◽  
Ruan Jing ◽  
Guangxia Shen
Keyword(s):  
Carbon Dots ◽  
Large Scale ◽  
High Efficiency ◽  
Reaction Medium ◽  
High Sensitivity ◽  
Heating Time ◽  
Serum Samples ◽  
Cancer Markers ◽  
N Doping ◽  
Doped Carbon Dots

Due to the dual role as an electron acceptor and an electron donor in solution, carbon dots (Cdots) have broad applications in environmental analysis, biological detection, and biosensors. Herein, we report a facile-green strategy for a large-scale synthesis of fluorescent N, P-doped carbon dots (N, P-Cdots) with an absolute quantum yield of 66.08% by a simple one-step thermal decomposition. Glucose was selected as a carbon precursor and tryptophan (Trp) as an N-doping and passivation reagent. Organic polar solvents with a high boiling point, i.e., ethylene glycol and glycerol, were used as the reaction medium, and phosphoric acid was employed as a P source and oxidation accelerator. It is shown that the emission wavelength of the N, P-Cdots can be tuned by adjusting the reaction conditions, such as mass ratio, heating time, temperature, and medium, without further passivation. Finally, advantage was taken of the superior fluorescent characteristics of N, P-Cdots to detect selectively and with high sensitivity a cancer marker, carcinoembryonic antigen (CEA), based on the fluorescent quenching mechanism. Additionally, CEA was also detected in human serum samples with high efficiency and RSD, further confirming that the proposed method has a good consistency and stability for supersensitive fluorimetric detection of cancer markers.

Oxygen-driven, high-efficiency production of nitrogen-doped carbon dots from alkanolamines and their application for two-photon cellular imaging

RSC Advances ◽  
2015 ◽  
Vol 5 (20) ◽  
pp. 15366-15373 ◽  
Author(s):  
Yaoping Hu ◽  
Jing Yang ◽  
Jiangwei Tian ◽  
Li Jia ◽  
Jun-Sheng Yu
Keyword(s):  
Carbon Dots ◽  
Large Scale ◽  
High Efficiency ◽  
Low Cost ◽  
Cellular Imaging ◽  
Nitrogen Doped ◽  
Two Photon ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

A novel oxygen-driven method has been developed for low-cost, large-scale, and high-efficiency production of nitrogen-doped carbon dots (N-C-dots) using various alkanolamines as both precursors and solvents.

Synthesis of novel cationic carbon dots and application to quantitative detection of K+ in human serum samples

2019 ◽  
Vol 43 (46) ◽  
pp. 17937-17940 ◽  
Author(s):  
Zideng Gao ◽  
Shunyi Wang ◽  
Zijun Xu ◽  
Jin Liu ◽  
Yuanfang Huang ◽  
...  
Keyword(s):  
Human Serum ◽  
Carbon Dots ◽  
High Sensitivity ◽  
Quantitative Detection ◽  
Serum Samples ◽  
Human Serum Samples

Novel cationic carbon dots were synthesized in a simple way and applied to detect K+ in human serum samples with ultra-high sensitivity.

scholarly journals Detection of Melamine Based on the Fluorescence Changes of Nitrogen-Doped Carbon Dots

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Wenzhi Yin ◽  
Chaoqun Ma ◽  
Tuo Zhu ◽  
Jiao Gu ◽  
Chun Zhu ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Detection Method ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Mercury Ions ◽  
Measuring Range ◽  
Nitrogen Doped ◽  
One Step ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

In order to determine the concentration of melamine, nitrogen-doped carbon dots (NCDs) were synthesized in one step as a fluorescent probe. Uric acid and diethylenetriamine were used as carbon source and nitrogen source, respectively. The experimental results showed that the fluorescence of NCDs can be quenched by mercury ions (Hg2+). Due to the strong coordination affinity between the carbon-nitrogen heterocyclic of melamine and Hg2+, part of Hg2+ coordinated with melamine when melamine was mixed with Hg2+. Then, the fluorescence of the added NCDs was quenched by the remaining Hg2+. Therefore, the concentration of melamine could be determined. The results show that the method has high sensitivity in wide measuring range that the linear ranges are 50–400 μg/L and 800–2500 μg/L, and the R2 is 0.997 and 0.988, respectively, with the limit of detection (LOD) of 21.76 μg/L. The NCDs are easy to fabricate, and the detection method is easy to implement. In this study, a new method for melamine detection was established, and the proposed method for melamine detection can provide some insights for food safety detection.

scholarly journals Colorimetric and Fluorescent Dual-Modality Sensing Platform Based on Fluorescent Nanozyme

2021 ◽  
Vol 9 ◽  
Author(s):  
Yejian Wan ◽  
Jingwen Zhao ◽  
Xiaochun Deng ◽  
Jie Chen ◽  
Fengna Xi ◽  
...  
Keyword(s):  
Large Scale ◽  
Hydrothermal Process ◽  
Single Layer ◽  
High Sensitivity ◽  
Single Layer Graphene ◽  
Peroxidase Mimic ◽  
Uniform Size ◽  
Dual Modality ◽  
Sensing Platform ◽  
N Doping

Compared with natural enzymes, nanozymes based on carbonaceous nanomaterials are advantages due to high stability, good biocompatibility, and the possibility of multifunctionalities through materials engineering at an atomic level. Herein, we present a sensing platform using a nitrogen-doped graphene quantum dot (NGQD) as a highly efficient fluorescent peroxidase mimic, which enables a colorimetric/fluorescent dual-modality platform for detection of hydrogen peroxide (H2O2) and biomolecules (ascorbic acid-AA, acid phosphatase-ACP) with high sensitivity. NGQD is synthesized using a simple hydrothermal process, which has advantages of high production yield and potential for large-scale preparation. NGQD with uniform size (3.0 ± 0.6 nm) and a single-layer graphene structure exhibits bright and stable fluorescence. N-doping and ultrasmall size endow NGQD with high peroxidase-mimicking activity with an obviously reduced Michaelis–Menten constant (Km) in comparison with natural horseradish peroxidase. Taking advantages of both high nanozyme activity and unique fluorescence property of NGQD, a colorimetric and fluorescent dual-modality platform capable of detecting H2O2 and biomolecules (AA, ACP) with high sensitivity is developed as the proof-of-concept demonstration. Furthermore, the mechanisms underlying the nanozyme activity and biosensing are investigated.

scholarly journals Green Hydrothermal Synthesis of N-doped Carbon Dots from Biomass Highland Barley for the Detection of Hg2+

Sensors ◽  
2019 ◽  
Vol 19 (14) ◽  
pp. 3169 ◽  
Author(s):  
Xie ◽  
Cheng ◽  
Liu ◽  
Han
Keyword(s):  
Carbon Dots ◽  
Limit Of Detection ◽  
Amorphous Structure ◽  
Water Soluble ◽  
Diameter Distribution ◽  
High Quantum Yield ◽  
N Doping ◽  
Ft Ir ◽  
Doped Carbon Dots ◽  
Highland Barley

Totally water-soluble N-doped Carbon dots (N-CDs) were synthesized by a green hydrothermal method from biomass using Highland barley as a carbon source and ethanediamine as nitrogen source. TEM and XRD showed the graphitic amorphous structure and narrow diameter distribution of these N-CDs. N-doping to the crystal lattice and carrying many hydrophilic groups on the surface of N-CDs were verified by XPS and FT-IR. The as-synthesized N-CDs emitted strong blue fluorescence at 480 nm and owned a relatively high quantum yield of 14.4%. The product also could sensitively and selectively detect Hg2+ ions in the range of 10–160 μM and the limit of detection was equal to 0.48 μM.

Development of N,S-doped carbon dots as a novel matrix for the analysis of small molecules by negative ion MALDI-TOF MS

RSC Advances ◽  
2016 ◽  
Vol 6 (82) ◽  
pp. 79043-79049 ◽  
Author(s):  
Yanwei Wang ◽  
Dan Gao ◽  
Yongli Chen ◽  
Guangnan Hu ◽  
Hongxia Liu ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Small Molecules ◽  
Carbon Dots ◽  
High Sensitivity ◽  
Negative Ion ◽  
Good Reproducibility ◽  
High Salt Tolerance ◽  
Small Molecule Analysis ◽  
Doped Carbon Dots ◽  
Tof Ms

The synthesized N,S-CDs was firstly used as a matrix for small molecule analysis with good reproducibility, high sensitivity and high salt tolerance.

How do nitrogen-doped carbon dots generate from molecular precursors? An investigation of the formation mechanism and a solution-based large-scale synthesis

2015 ◽  
Vol 3 (27) ◽  
pp. 5608-5614 ◽  
Author(s):  
Yaoping Hu ◽  
Jing Yang ◽  
Jiangwei Tian ◽  
Jun-Sheng Yu
Keyword(s):  
Formation Mechanism ◽  
Formation Process ◽  
Carbon Dots ◽  
Large Scale ◽  
Molecular Precursors ◽  
Nitrogen Doped ◽  
Scalable Synthesis ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon ◽  
Fluorescent Carbon Dots

A solution-based method is developed for rapid and scalable synthesis of highly fluorescent carbon dots in a monoethanolamine system, and an interesting formation process of carbon dots is directly observed.

N-Doped carbon dots: green and efficient synthesis on a large-scale and their application in fluorescent pH sensing

2017 ◽  
Vol 41 (19) ◽  
pp. 10607-10612 ◽  
Author(s):  
Xiaoxia Liu ◽  
Jinrong Liu ◽  
Baozhan Zheng ◽  
Lei Yan ◽  
Jianyuan Dai ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Large Scale ◽  
Ph Sensitive ◽  
Ph Sensing ◽  
Efficient Synthesis ◽  
Nitrogen Doped ◽  
Sensitive Property ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

A green and efficient anhydrous method was developed to synthesize nitrogen doped carbon dots (N-CDs) on a large-scale, and the N-CDs exhibited excellent reversible pH-sensitive property.

scholarly journals N-Doped Carbon Dots Derived from Melamine and Triethanolamine for Selective Sensing of Fe3+ Ions

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Sathishkumar Munusamy ◽  
Sathish Sawminathan ◽  
Thanigaivelan Arumugham ◽  
Maura Casales Díaz ◽  
Srinivas Godavarthi ◽  
...  
Keyword(s):  
Water Samples ◽  
Carbon Dots ◽  
Limit Of Detection ◽  
Blue Emission ◽  
World Health ◽  
Selective Determination ◽  
N Doping ◽  
Health Organization ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

This work reports nitrogen-doped carbon dots (NCDs) as a selective sensing probe to detect Fe3+ in water samples. NCD probes were synthesized via solvothermal method using nitrogen-rich melamine and triethanolamine as precursors. Properties of the resulting NCDs were studied using different characterization techniques, through which N-doping was confirmed. The quantum yield of obtained NCDs was measured to be 21%. When excited at 370 nm, the excellent blue emission property makes this probe adoptable for selectively sensing Fe3+ in practical water samples. The limit of detection (LOD) was identified as 216 nM with a good linear range between the concentrations of 0.2-2 μM. The obtained LOD is far less than the World Health Organization (WHO) permissible limits of Fe3+ in water. Interference studies reveal that the presence of other competing ions did not alter the sensing of Fe3+, even at the presence of 10 equivalents which indicates the high selectivity of NCDs towards Fe3+. The reversibility studies showed that adding a cheap and readily available EDTA ligand to the NCD results in fluorescence regeneration, leading to exceptional reusability for the detection of Fe3+. So, the synthesized NCDs can be used as a suitable probe for the selective determination of Fe3+ in real water samples.

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